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Huy, N.D.,Nguyen, C.L.,Park, H.S.,Loc, N.H.,Choi, M.S.,Kim, D.H.,Seo, J.W.,Park, S.M. Society for Bioscience and Bioengineering, Japan ; 2016 Journal of bioscience and bioengineering Vol.121 No.2
<P>The cDNA encoding a putative glycoside hydrolase family 5, which has been predicted to be an endoglucanase (PcEg5A), was cloned from Phanerochaete chrysosporium and expressed in Pichia pastoris. PcEg5A contains a carbohydrate-binding domain and two important amino acids, E209 and E319, playing as proton donor and nucleophile in substrate catalytic domain. SDS-PAGE analysis indicated that the recombinant endoglucanase 5A (rPcEg5A) has a molecular size of 43 kDa which corresponds with the theoretical calculation. Optimum pH and temperature were found to be 4.5-6.0, and 50 degrees C-60 degrees C, respectively. Moreover, rPcEg5A exhibited maximal activity in the pH range of 3.0-8.0, whereas over 50% of activity still remained at 20 degrees C and 80 degrees C. rPcEg5A was stable at 60 degrees C for 12 h incubation, indicating that rPcEg5A is a thermostable enzyme. Manganese ion enhanced the enzyme activity by 77%, indicating that rPcEg5A is a metal dependent enzyme. The addition of rPcEg5A to cellobiase (cellobiohydrolase and beta-glucosidase) resulted in a 53% increasing saccharification of NaOH-pretreated barley straw, whereas the glucose release was 47% higher than that cellobiase treatment alone. Our study suggested that rPcEg5A is an enzyme with great potential for biomass saccharification. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.</P>
Lipid N-formylation Occurs During Fixation with Formalin
김민식,Min Jung Kim,임희진,Muwoong Kim,Yu Ri Choi,Thy N. C. Nguyen,Seung Cheol Park,Kwang Pyo Kim,Junyang Jung 사단법인 한국질량분석학회 2022 Mass spectrometry letters Vol.13 No.2
Human tissues and organs can be preserved intact by fixation with formalin for the future analysis of biomolecules of interest. With the advances in high-throughput methods, numerous protocols have been developed and optimized to attain the most pathophysiological information out of biomolecules, including RNA and proteins, in formalin-fixed samples. However, there is no systematic study to examine the effects of formalin fixation on the lipidome of biological samples in a global fashion. In this study, we conducted a mass spectrometry-based analysis to survey the alteration in the lipidome of mice brains by fixation methods. A total of 308 lipids were quantitatively measured using triple quadrupole mass spectrometry. We found that most were unchanged after formalin fixation except for a few lipid classes such as phosphatidylethanolamine.
Study of Nanostructured Polymeric Composites and Hybrid Layers Used for Light-Emitting Diodes
N. N. Dinh,L. H. Chi,T. T. C. Thuy,D. V. Thanh,T. P. Nguyen 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.2
Nanocomposite polymeric thin lms of poly(2-methoxy-5-(2'-ethyl hexyloxy)phenylene vinylene) (MEH-PPV) with nano-crystalline titanium oxide (nc-TiO2) and polycarbazole (PVK) with cadmium selenide (CdSe) were fabricated by spin-coating. A 20-nm-thick nanocrystalline (molyb-denum oxide) MoO3 layer prepared by using thermal oxidation was also spin-coated with a PVK film. A study of photoluminescence (PL) spectra of the polymers and nanocomposites showed an enhancement in the photoluminescence from the nanocomposites. Moreover, MEH-PPV+nc-TiO2 composites exhibited a blue-shift of the PL spectrum as compared to that of the polymer film. The current-voltage characteristics of the Mo/nc-MoO3/PVK/Al-Au structures show that a semitransparent metallic coating or a transparent polymer can serve as a cathode in devices with enhanced charge carrier injection.
Production of steviol from steviol glucosides using β-glycosidase from Sulfolobus solfataricus
Nguyen, T.T.H.,Kim, S.B.,Kim, N.M.,Kang, C.,Chung, B.,Park, J.S.,Kim, D. IPC Science and Technology Press ; Elsevier Scienc 2016 Enzyme and microbial technology Vol.93 No.-
<P>Steviol is a diterpene isolated from the plant Stevia rebaudiana that has a potential role as an antihyperglycemic agent by stimulating insulin secretion from pancreatic beta cells and also has significant potential to diminish the renal clearance of anionic drugs and their metabolites. In this study, the lacS gene, which encodes a thermostable beta-glycosidase (SSbgly) enzyme from the extremely thermoacidophillic archaeon Sulfolobus solfataricus, was cloned and expressed in E coil Rossetta BL21(DE3)pLyS using lactose as an inducer. Through fermentation, SSbgly was expressed as a 61 kDa protein with activity of 24.3 U/mg and the OD600 of 23 was reached after 18 h induction with 10 mM lactose. Purified protein was obtained by Ni-Sepharose chromatography with a yield of 92.3%. SSbgly hydrolyzed steviol glycosides to produce steviol with a yield of 99.2%. The optimum conditions for steviol production were 50 U/ml SSbgly and 90 mg/ml Ste at 75 degrees C as determined by the response surface method. (C) 2016 Elsevier Inc. All rights reserved.</P>
A review on the oil-soluble dispersed catalyst for slurry-phase hydrocracking of heavy oil
Nguyen, M.T.,Nguyen, N.T.,Cho, J.,Park, C.,Park, S.,Jung, J.,Lee, C.W. Korean Society of Industrial and Engineering Chemi 2016 Journal of industrial and engineering chemistry Vol.43 No.-
<P>Nowadays, unconventional-oil becomes a potential candidate for satisfying the world's energy demands due to the scarcity of other energy sources. However, it contains many impurities, such as heavy metal, sulfur-compounds, and nitrogen-compounds, and leads to quick deactivation of catalyst, high coke formation, and large pressure drop during the operation of a fixed bed or E-bed. Slurry phase hydrocracking (SHC) with the presence of oil-dispersed catalyst has been proven to be the best solution to overcome those problems. In oil-dispersed, the metal precursor, dispersion ability, and additive strongly affect the catalyst performance, and are all reviewed in this paper. (C) 2016 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.</P>