Rapid blockade of telomerase activity and tumor cell growth by the DPL lipofection of ribbon antisense to hTR

Bajpai, Arun K,Park, Jeong-Hoh,Moon, Ik-Jae,Kang, Hyungu,Lee, Yun-Han,Doh, Kyung-Oh,Suh, Seong-Il,Chang, Byeong-Churl,Park, Jong-Gu Nature Publishing Group 2005 Oncogene Vol.24 No.43

Ribbon antisense (RiAS) to the hTR RNA, a component of the telomerase complex, was employed to inhibit telomerase activity and cancer cell growth. The antisense molecule, hTR-RiAS, combined with enhanced cellular uptake was shown to effectively inhibit telomerase activity and cause rapid cell death in various cancer cell lines. When cancer cells were treated with hTR-RiAS, the level of hTR RNA was reduced by more than 90% accompanied with reduction in telomerase activity. When checked for cancer cell viability, cancer cell lines treated with hTR-RiAS using DNA+Peptide+Lipid complex showed 70–80% growth inhibition in 3 days. The reduced cell viability was due to apoptosis as the percentage of cells exhibiting the sub-G<SUB>0</SUB> arrest and DNA fragmentation increased after antisense treatment. Further, when subcutaneous tumors of a colon cancer cell line (SW480) were treated intratumorally with hTR-RiAS, tumor growth was markedly suppressed with almost total ablation of hTR RNA in the tumor tissue. Cells in the tumor tissue were also found to undergo apoptosis after hTR-RiAS treatment. These results suggest that hTR-RiAS is an effective anticancer reagent, with a potential for broad efficacy to diverse malignant tumors.Oncogene (2005) 24, 6492–6501. doi:10.1038/sj.onc.1208731; published online 15 August 2005

Effects of Soil Amendments Application on Growth of Rice Cultivated in Soils Polluted with Heavy Metal(loid) and on the As and Cd Content in Brown Rice

Ji-Hyock Yoo,Sang-Won Park,Won-Il Kim,Sang-Beom Lee,Kyeong-Seok Oh,Byeong-Churl Moon,Sung-Chul Kim 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.6

Heavy metal(loid) contamination of rice is the main issue in abandoned metal mine area with regard to food safety. A field study was conducted in mine area to see if soil amendments treatment including calcium superphosphate, sulfur, steel slag and S-containing fertilizer could reduce As and/or Cd content in rice grain and increase the growth of rice. The As content in brown rice reduced to 60% compared to the control only in 7.0 Mg ha<SUP>-1</SUP> of steel slag treatment. Cd reduction in rice was thought to be not the effect of amendments but the result from the difference in growth and development of rice plant and this could be ascribed to low soil Cd availability to rice plant. Compared with control, increased rice yield of cultivar Hwanggeumnuri was 1.3~2.2 and 1.5 Mg ha<SUP>-1</SUP> in calcium super phosphate and S-containing fertilizer treatment, respectively and the trend was also observed in cultivar Ungwang. However, steel slag treatment reduced the Ungwang yield by 0.4~0.9 Mg ha<SUP>-1</SUP>. Future work will be needed to establish the agricultural measure with which secure the safety and yield of rice simultaneously.

Identification of Transition Characteristics and Bio-concentration Factors of Heavy Metal (loid)s in the Selected Perennial Root Medicinal Plants

Won-Il Kim,Hyun Myung Noh,Chang-Oh Hong,Da-Young Kim,Kwon-Rae Kim,Kyeong-Seok Oh,Byeong-Churl Moon,Ji-Young Kim 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.4

This study was conducted to identify transition characteristics of arsenic (As), cadmium (Cd), and lead (Pb) and to calculate bio-concentration factors (BCF) in the three perennial root medicinal plants, namely Codonopsis lanceolata (Deoduck), Platycodon grandiflorum (Balloon flower) and Panax ginseng (Korean ginseng) grown in major medicinal plant producing districts in Korea. Average BCF values ranged from 0.009~0.029 in As, 0.334~1.453 in Cd, and 0.021~0.023 in Pb in three perennial root medicinal plants. The BCF values increased in the order of ginseng (0.029) > deodeok (0.012) > balloon flower (0.009) for As, balloon flower (1.453) > deodeok (0.685) > ginseng (0.334) for Cd, and ginseng (0.023) > deodeok (0.022) > balloon flower (0.021) for Pb. The BCF values calculated in this study will be useful for predicting the uptake of heavy metal (loid)s. Further study on uptake and accumulation mechanism of toxic metal (loid)s by agricultural products is required to assess the human health risk associated with soil contamination.

Effects of Soil Amendments Application on Growth of Rice Cultivated in Soils Polluted with Heavy Metal(loid) and on the As and Cd Content in Brown Rice

Yoo, Ji-Hyock,Park, Sang-Won,Kim, Won-Il,Lee, Sang-Beom,Oh, Kyeong-Seok,Moon, Byeong-Churl,Kim, Sung-Chul 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.6

Heavy metal(loid) contamination of rice is the main issue in abandoned metal mine area with regard to food safety. A field study was conducted in mine area to see if soil amendments treatment including calcium superphosphate, sulfur, steel slag and S-containing fertilizer could reduce As and/or Cd content in rice grain and increase the growth of rice. The As content in brown rice reduced to 60% compared to the control only in $7.0Mg\;ha^{-1}$ of steel slag treatment. Cd reduction in rice was thought to be not the effect of amendments but the result from the difference in growth and development of rice plant and this could be ascribed to low soil Cd availability to rice plant. Compared with control, increased rice yield of cultivar Hwanggeumnuri was 1.3~2.2 and $1.5Mg\;ha^{-1}$ in calcium super phosphate and S-containing fertilizer treatment, respectively and the trend was also observed in cultivar Ungwang. However, steel slag treatment reduced the Ungwang yield by $0.4{\sim}0.9Mg\;ha^{-1}$. Future work will be needed to establish the agricultural measure with which secure the safety and yield of rice simultaneously.

비소 오염 취약논토양에서의 쌀 중 중금속의 생물농축계수 산정

김원일(Won-Il Kim),류지혁(Ji-Hyock Yoo),박상원(Sang-Won Park),오경석(Kyeong-Seok Oh),문병철(Byeong-Churl Moon) 한국토양비료학회 2017 한국토양비료학회 학술발표회 초록집 Vol.2017 No.5

Identification of Transition Characteristics and Bio-concentration Factors of Heavy Metal (loid)s in the Selected Perennial Root Medicinal Plants

Kim, Won-Il,Noh, Hyun Myung,Hong, Chang-Oh,Kim, Da-Young,Kim, Kwon-Rae,Oh, Kyeong-Seok,Moon, Byeong-Churl,Kim, Ji-Young 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.4

This study was conducted to identify transition characteristics of arsenic (As), cadmium (Cd), and lead (Pb) and to calculate bio-concentration factors (BCF) in the three perennial root medicinal plants, namely Codonopsis lanceolata (Deoduck), Platycodon grandiflorum (Balloon flower) and Panax ginseng (Korean ginseng) grown in major medicinal plant producing districts in Korea. Average BCF values ranged from 0.009~0.029 in As, 0.334~1.453 in Cd, and 0.021~0.023 in Pb in three perennial root medicinal plants. The BCF values increased in the order of ginseng (0.029) > deodeok (0.012) > balloon flower (0.009) for As, balloon flower (1.453) > deodeok (0.685) > ginseng (0.334) for Cd, and ginseng (0.023) > deodeok (0.022) > balloon flower (0.021) for Pb. The BCF values calculated in this study will be useful for predicting the uptake of heavy metal (loid)s. Further study on uptake and accumulation mechanism of toxic metal (loid)s by agricultural products is required to assess the human health risk associated with soil contamination.

Prediction of Arsenic Uptake by Rice in the Paddy Fields Vulnerable to Arsenic Contamination

Seul Lee,Dae-Won Kang,Hyuck-Soo Kim,Ji-Hyock Yoo,Sang-Won Park,Kyeong-Seok Oh,Il Kyu Cho,Byeong-Churl Moon,Won-Il Kim 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.2

There is an increasing concern over arsenic (As) contamination in rice. This study was conducted to develope a prediction model for As uptake by rice based on the physico-chemical properties of soil. Soil and brown rice samples were collected from 46 sites in paddy fields near three different areas of closed mines and industrial complexes. Total As concentration, soil pH, Al oxide, available phosphorus (avail-P), organic matter (OM) content, and clay content in the soil samples were determined. Also, 1.0 N HCl, 1.0 M NH₄NO₃, 0.01 M Ca(NO₃)₂, and Mehlich 3 extractable-As in the soils were measured as phytoavailable As concentration in soil. Total As concentration in brown rice samples was also determined. Relationships among As concentrations in brown rice, total As concentrations in soils, and selected soil properties were as follows: As concentration in brown rice was negatively correlated with soil pH value, where as it was positively correlated with Al oxide concentration, avail-P concentration, and OM content in soil. In addition, the concentration of As in brown rice was statistically correlated only with 1.0 N HCl-extractable As in soil. Also, using multiple stepwise regression analysis, a modelling equation was created to predict As concentration in brown rice as affected by selected soil properties including soil As concentration. Prediction of As uptake by rice was delineated by the model [As in brown rice = 0.352 + 0.00109 <SUP>*</SUP> HCl extractable As in soil + 0.00002 <SUP>*</SUP> Al oxide + 0.0097 <SUP>*</SUP> OM + 0.00061 <SUP>*</SUP> avail-P – 0.0332 <SUP>*</SUP> soil pH] (R = 0.714<SUP>***</SUP>). The concentrations of As in brown rice estimated by the modelling equation were statistically acceptable because normalized mean error (NME) and normalized root mean square error (NRMSE) values were -0.055 and 0.2229, respectively, when compared with measured As concentration in the plant.

Prediction of Arsenic Uptake by Rice in the Paddy Fields Vulnerable to Arsenic Contamination

Lee, Seul,Kang, Dae-Won,Kim, Hyuck-Soo,Yoo, Ji-Hyock,Park, Sang-Won,Oh, Kyeong-Seok,Cho, Il Kyu,Moon, Byeong-Churl,Kim, Won-Il 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.2

There is an increasing concern over arsenic (As) contamination in rice. This study was conducted to develope a prediction model for As uptake by rice based on the physico-chemical properties of soil. Soil and brown rice samples were collected from 46 sites in paddy fields near three different areas of closed mines and industrial complexes. Total As concentration, soil pH, Al oxide, available phosphorus (avail-P), organic matter (OM) content, and clay content in the soil samples were determined. Also, 1.0 N HCl, 1.0 M $NH_4NO_3$, 0.01 M $Ca(NO_3)_2$, and Mehlich 3 extractable-As in the soils were measured as phytoavailable As concentration in soil. Total As concentration in brown rice samples was also determined. Relationships among As concentrations in brown rice, total As concentrations in soils, and selected soil properties were as follows: As concentration in brown rice was negatively correlated with soil pH value, where as it was positively correlated with Al oxide concentration, avail-P concentration, and OM content in soil. In addition, the concentration of As in brown rice was statistically correlated only with 1.0 N HCl-extractable As in soil. Also, using multiple stepwise regression analysis, a modelling equation was created to predict As concentration in brown rice as affected by selected soil properties including soil As concentration. Prediction of As uptake by rice was delineated by the model [As in brown rice = 0.352 + $0.00109^*$ HCl extractable As in soil + $0.00002^*$ Al oxide + $0.0097^*$ OM + $0.00061^*$ avail-P - $0.0332^*$ soil pH] ($R=0.714^{***}$). The concentrations of As in brown rice estimated by the modelling equation were statistically acceptable because normalized mean error (NME) and normalized root mean square error (NRMSE) values were -0.055 and 0.2229, respectively, when compared with measured As concentration in the plant.

카드뮴 함량에 관한 벼 품종 간의 유전적 다양성

이상범 ( Sang-beom Lee ),김경진 ( Gyeong-jin Kim ),최부웅 ( Buung Choi ),류지혁 ( Ji-hyock Yoo ),오경석 ( Kyeong-seok Oh ),문병철 ( Byeong-churl Moon ),박용진 ( Yong-jin Park ),박상원 ( Sang-won Park ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-

토양 중에 CdS 형태로 존재하는 카드뮴은 토양이 환원상태일 때 Cd²⁺로 전환되어 벼에 흡수된 다. 벼의 카드뮴(Cd) 흡수에 관여하는 유전자는 OsIRT1과 OsNramp5, OsHMA3, OsCDT4 등이 있다. 카드뮴은 OsIRT, OsNramp5, OsHMA family에 의해 뿌리의 외피에서 내피로 이동하며, 물관 부를 거쳐 체관부에 축적된 카드뮴은 다시 OsLCT1 유전자에 의해 종실로 전이 된다. 일반적으로 벼의 형질은 한개의 유전자가 아닌 복수의 유전자가 상호작용을 통해 발현 된다. 벼의 카드뮴흡수 메커니즘에 관여하는 유전자를 탐색하고 궁극적으로 카드뮴 저흡수 품종을 육성하기 위해 본 연구에서는 벼 유전자원 245점을 광산인근 지역에서 재배하여 현미 내 카드뮴 함량을 유도결합플 라즈마질량분석기(ICP-MS, Agilent 7700E)로 정량 분석하였다. 그리고 카드뮴 함량을 표현형 데이터로 하여 벼 품종의 SNP 간 연관성을 찾기 위해 전장유전체연관분석(Genome Wide Association Studies, GWAS)을 하였다. 전장유전체연관분석 결과 염색체 8번의 25299634 위치에서 -log (P) 값 이 5.37로 high peak를 보였고, 염색체 1번의 30402852 위치에서는 -log (P) 값이 5.17로 나타났다. 카드뮴 연관 후보유전자를 ±2Mb 범위에서 탐색한 결과 염색체 8번과 1번에서 각각 51개와 49개의 후보유전자가 확인되었다. 확인된 후보유전자는 반수체(haplotype)의 염기서열과 아미노산의 변화를 분석하여 벼 품종 간의 유전적 다양성을 확인하였다. 카드뮴과 연관된 후보 유전자의 기능을 밝힌 본 연구 결과는 향후 분자육종분야에서 안전한 벼 품종 육성을 위한 기초자료와 식물 환경복원 분야에서 중금속 저감을 위한 자료로 활용되어 질 것으로 기대된다.

비소 오염지에서 재배된 벼 선발집단의 미네랄 함량 차이

최부웅 ( Buung Choi ),이상범 ( Sang-beom Lee ),김경진 ( Gyeong-jin Kim ),류지혁 ( Ji-hyock Yoo ),오경석 ( Kyeong-seok Oh ),문병철 ( Byeong-churl Moon ),김욱한 ( Wook-han Kim ),정남진 ( Nam-jin Chung ),박상원 ( Sang-won Park ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-

세계 3대 식량작물로 전 세계 인구의 50% 이상이 섭취하고 있는 쌀은 주로 아시아 지역에서 재배되며 미네랄, 비타민 등이 풍부하고 영양적 가치가 높아 우리나라에서도 오래 전부터 주식으로 이용해 왔다. 현재까지 우리나라에서 육성 및 재배된 벼는 95% 이상이 자포니카(japnonica type) 품종으로 이루어져 있다. 최근 급속한 기후변화에 따라 향후 30~50년 뒤에는 대부분의 품종이 인 디카(indica type) 품종으로 대체될 것으로 전망되고 있다. 그런데 인디카 품종은 상대적으로 자포니카 품종 보다 비소 등 중금속뿐만 아니라 다양한 미네랄을 많이 흡수 한다는 연구 결과들이 보고되고 있다. 벼 선발집단 295종을 비소 등 중금속이 오염된 농경지에서 재배한 다음 미네랄 27종을 ICP-MS로 정량분석한 결과 전반적으로 인디카 품종들이 자포니카 품종등 다른 생태형 품종들 보다 비소를 포함한 중금속뿐만 아니라 기능성 미네랄들의 함량이 높은 것으로 나타났다. 향후 정확한 미네랄 흡수·축적에 대한 메카니즘과 미네랄 네트워크 분석이 필요한 것으로 판단된다.