Versatile Surface-coatable IR825-conjugated Fluorescent Polymer as a Dual-functional Biosensor for Synergistic Detection and Eradication of Bacteria

( Akhmad Irhas Robby ),( Sung Young Park ) 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.1

Recyclable metal nanoparticle-immobilized polymer dot on montmorillonite for alkaline phosphatase-based colorimetric sensor with photothermal ablation of Bacteria

Robby, Akhmad Irhas,Park, Sung Young Elsevier 2019 Analytica chimica acta Vol.1082 No.-

<P><B>Abstract</B></P> <P>Development of simultaneous bacteria detection and eradication with simple, rapid, and reusable material is important in addressing bacterial contamination issues. In this study, we utilized the expression of alkaline phosphatase (ALP) from bacteria to design fluorescence ON/OFF system for bacteria detection, also using metal oxide nanoparticle for obtaining antibacterial activity and recyclability. The fluorescent-based biosensor with antibacterial activity was prepared by intercalating ALP-sensitive polymer dot (PD) containing β-cyclodextrin (β-CD) onto montmorillonite (MMT) as loading matrix <I>via</I> ionic exchange reaction, followed by immobilization of magnetic iron oxide (Fe<SUB>3</SUB>O<SUB>4</SUB>) and NIR-responsive cesium tungsten oxide (CsWO<SUB>3</SUB>). The PD-βCD-MMT/Fe<SUB>3</SUB>O<SUB>4</SUB>–CsWO<SUB>3</SUB> nanocomposite exhibited strong fluorescence intensity, which was quenched in the presence of bacterial ALP (0–1000 U/L) due to hydrolysis of <I>p</I>-nitrophenyl phosphate (NPP) into <I>p</I>-nitrophenol (NP) in the hydrophobic site of β-CD. Furthermore, the nanocomposite could detect both gram-negative <I>Escherichia coli</I> and gram-positive <I>Staphylococcus aureus</I> in the range of 10<SUP>1</SUP>–10<SUP>7</SUP> CFU/mL (LOD 5.09 and 4.62 CFU/mL, respectively), and showed high antibacterial activity against bacteria by generating photothermal heat under 5 min NIR irradiation, causing damage to bacterial cells. This material also demonstrated recyclability <I>via</I> magnetic field exposure due to the presence of Fe<SUB>3</SUB>O<SUB>4.</SUB> In addition, the fluorescence can be recovered following pH shock and re-conjugation of β-CD molecules. After 4 cycles, nanocomposite still showed stable photothermal effects and fluorescence-based bacteria detection. Thus, this reusable material offers promising approach for simultaneous bacteria detection and killing, which is simple, rapid, and effective.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Reusable material was designed for fluorometric sensing and photothermolysis of bacteria. </LI> <LI> The fluorescence ON/OFF sensing system was depended on the bacterial ALP activity. </LI> <LI> The LOD of fluorescence-based bacteria detection showed below 10<SUP>1</SUP> CFU/mL. </LI> <LI> Hybrid nanocomposite showed ±100% killing efficiency after 5 min NIR irradiation. </LI> <LI> Antibacterial/bacteria sensing showed excellent performance even after 4 cycles. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Mitochondria-targeted ROS- and GSH-responsive diselenide-crosslinked polymer dots for programmable paclitaxel release

김슬기,Akhmad Irhas Robby,이병찬,이기백,박성영 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.99 No.-

Enhancing therapeutic efficacy of drugs from reactive oxygen species (ROS) and glutathione (GSH)-responsive matrix and minimizing toxic effects on normal cells remains a challenge in programmableanticancer drug delivery. Herein, ROS- and GSH-responsive paclitaxel (PTX)-loaded polymer dot (PD)with mitochondria-targeting capability was designed by constructing diselenide linkage andtriphenylphosphonium (TPP) for tunable PTX release andfluorescence for cancer theranostics. PDTPPnanocarrier could improve the PTX stability after loading (PD-TPP(PTX)), and the cleavage ofdiselenide bond in the presence of H2O2 and GSH triggered the controllable PTX release, providing higherfluorescence intensity. As the levels of H2O2 and GSH are higher in cancer cells compared to normal cells,PTX was selectively released from PD-TPP in cancer cells, reducing cell viability ( 25%) and causingenhanced apoptosis of cancer cells compared to normal cells. The PD-TPP(PTX) selectivity was alsoreflected by distinctfluorescence intensity in HeLa and PC-3 cells (cancer) compared to CHO-K1 cells(normal). Furthermore, conjugated TPP promoted the PD-TPP(PTX) accumulation in mitochondria due tospecific targeting of TPP towards mitochondria, allowing PTX release in mitochondria of cancer cells. Hence, this approach could be a potential strategy to enhance therapeutic efficacy of cancer drugs andminimize the side effects on normal cells.

Photoluminescence-tunable fluorescent carbon dots-deposited silver nanoparticle for detection and killing of bacteria

Roh, Sang Gyu,Robby, Akhmad Irhas,Phuong, Pham Thi My,In, Insik,Park, Sung Young Elsevier 2019 Materials Science and Engineering C Vol.97 No.-

<P><B>Abstract</B></P> <P>Innovative methods to detect and kill pathogenic bacteria have a pivotal role in the eradication of infectious diseases and the prevention of the growth of antibiotic-resistant bacteria. The combination of fluorescent carbon dots (FCDs) with silver nanoparticles (AgNPs) is an effective material for synergic detection and antimicrobial activity determination. However, the fluorescence quenching of the FCDs owing to an interaction with AgNP is a major limitation. In this study, we designed a system to utilize poly(vinylpyrrolidone) (PVP) and catechol chemistry (PVP@Ag:FCD) in order to avoid the fluorescence quenching of the FCD-AgNP combination due to Forster Resonance Energy Transfer (FRET). PVP@Ag:FCD exhibited bright fluorescence, which can be used for bacterial detection, through the promotion of electrostatic binding with the negatively-charged bacterial surface and generation of fluorescence quenching due to aggregation-induced quenching. Furthermore, the presence of silver nanoparticles in PVP@Ag:FCD produced an excellent bacteria killing efficiency against <I>E. coli</I> and <I>S. aureus</I>, even at low concentrations (0.1 mg/mL). In contaminated river water, the PVP@Ag:FCD system showed a simple, highly sensitive, and effective performance for both the detection and eradication of bacteria. Therefore, this system offers an auspicious method for the future detection and killing of bacteria.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An FCD and AgNP-based material was designed for synergistic bacteria detection and killing. </LI> <LI> PVP@Ag:FCD showed that our approach can avoid Ag-induced fluorescence quenching of FCD. </LI> <LI> Fluorescence of PVP@Ag:FCD was quenched when treated with bacteria. </LI> <LI> PVP@Ag:FCD showed a great bacteria killing efficiency against both <I>E. coli</I> and <I>S. aureus</I>. </LI> <LI> This system offers promising strategy to combine biosensors with antibacterial activity. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Alkaline phosphatase-responsive fluorescent polymer probe coated surface for colorimetric bacteria detection

Kang, Eun Bi,Mazrad, Zihnil Adha Islamy,Robby, Akhmad Irhas,In, Insik,Park, Sung Young Elsevier 2018 European polymer journal Vol.105 No.-

<P><B>Abstract</B></P> <P>The present study aimed to develop an enzymatic colorimetric method using a surface-adsorbing biosensor to detect and kill bacteria in a single, simple, and rapid assay. The phosphorylated fluorescent probe 2-hydroxychalcone (HCAP) conjugated with an adhesive cationic polymer was designed (HCAP-PVP), which yielded greenish-yellow emission in aqueous buffer. Upon introduction of <I>Escherichia coli</I> and <I>Staphylococcus aureus</I>, the phosphate group inside the HCAP was cleaved by endogenous alkaline phosphatase (ALP) and the greenish-yellow emission ratiometrically changed to deep-red emission. This biosensor system detected bacteria over a wide range of bacterial densities (10<SUP>1</SUP>–10<SUP>7</SUP> colony-forming units/mL) after 60 min, with similar bacterial detection abilities between aqueous- and solid-phase assays. Furthermore, the presence of a quaternary ammonium of dodecane in this system displayed efficient antibacterial activity because of the change in cellular hydrophobic interactions, which enabled this material to act as a dual sensor and killing material. Thus, this system is a novel, rapid, and simple enzymatic sensor with high sensitivity that can be used as a solid-based platform to detect and directly eliminate bacteria.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A colorimetric method was designed for detection of bacteria based on ALP activity. </LI> <LI> HCAP-conjugated adhesive cationic polymer showed fluorescent changes in response to ALP. </LI> <LI> Quaternary ammonium of dodecane in this system provided antibacterial activity. </LI> <LI> This system can be used for both aqueous- and solid-phase assays. </LI> <LI> This sensor showed excellent bacteria detection ability and antibacterial activity. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>