Characterization of the N - ras proto - oncogene by PCR - cDNA amplification

Kim, Young Soo,Min, Kyung Rak,Gerald N Wogan 한국유전학회 1989 Genes & Genomics Vol.11 No.4

A 600-base pair cDNA containing the entire rat N-ras coding exonic sequence was amplified by a transcript-PCR method. Sequence comparison of mammalian N-ras genes revealed that the rat N-ras proto-oncogene shared extensive sequence homology with human and mouse N-ras proto-oncogenes. The homology of N-ras in human, mouse, and rat was 90 to 95% in nucleotide sequence. Rat N-ras exhibited 4 amino acid differences when compared to mouse N-ras, 3 of which were in the C-terminal region encoded by exon IV. An evolutionary divergence at position 69 was also found, in which rat N-ras contained glycine but other known mammalian ras proteins including H-, K-, and N-ras have aspartate at position 69 in this highly conserved region. Starting from total RNA derived from the liver of Fischer rat, a double strand cDNA containing two unique endonuclease sites in its termini was obtained within 5 hrs. The terminal endonuclease sites were used for direct cloning into M13 to obtain a library enriched for N-ras proto-oncogene. Screening assay of plaques and sequence analysis of individual cloned cDNA product were used to evaluate the efficency and fidelity of this cDNA amplification-direct M13 cloning procedure. The target N-ras cDNA clones were detected in 5% of the total screened plaques and no sequence errors introduced by the in vitro amplification procedure were observed. This method proved satisfactory for obtaining a complete cDNA sequence of the N-ras proto-oncogene from nanogram quantities of the total liver RNA.

Nitrogen-Doped Carbon Nanoparticles by Flame Synthesis as Anode Material for Rechargeable Lithium-Ion Batteries

Bhattacharjya, Dhrubajyoti,Park, Hyean-Yeol,Kim, Min-Sik,Choi, Hyuck-Soo,Inamdar, Shaukatali N.,Yu, Jong-Sung American Chemical Society 2014 Langmuir Vol.30 No.1

<P>Nitrogen-doped turbostratic carbon nanoparticles (NPs) are prepared using fast single-step flame synthesis by directly burning acetonitrile in air atmosphere and investigated as an anode material for lithium-ion batteries. The as-prepared N-doped carbon NPs show excellent Li-ion stoarage properties with initial discharge capacity of 596 mA h g<SUP>–1</SUP>, which is 17% more than that shown by the corresponding undoped carbon NPs synthesized by identical process with acetone as carbon precursor and also much higher than that of commercial graphite anode. Further analysis shows that the charge–discharge process of N-doped carbon is highly stable and reversible not only at high current density but also over 100 cycles, retaining 71% of initial discharge capacity. Electrochemical impedance spectroscopy also shows that N-doped carbon has better conductivity for charge and ions than that of undoped carbon. The high specific capacity and very stable cyclic performance are attributed to large number of turbostratic defects and N and associated increased O content in the flame-synthesized N-doped carbon. To the best of our knowledge, this is the first report which demonstrates single-step, direct flame synthesis of N-doped turbostratic carbon NPs and their application as a potential anode material with high capacity and superior battery performance. The method is extremely simple, low cost, energy efficient, very effective, and can be easily scaled up for large scale production.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2014/langd5.2014.30.issue-1/la403366e/production/images/medium/la-2013-03366e_0006.gif'></P>

Flow cytometric DNA content study on Uterine Leiomyomatous tumors

Chough, Soo Yong,Lee, Kap N. 고려대학교 의과대학 1993 고려대 의대 잡지 Vol.30 No.2

종양에서 세포의 비정상적인 분열산은 종양의 한 특징으로 생각되어 왔다. 이러한 종양의 한 특징인 세포의 분열상을 연구하는 여러 방법중 최근에 소개된 유세포측정법을 이용한 세포의 분열상에 대한 연구가 활발하다. 그러나 대부분 epithelial세포에 관한 연구 논문들이고 sarcomatoid세포에 관한 연구는 드물다. 이에 본 연구자들은 고려대학교 부속병원들에서 자궁 절제술로 제거된 평활근 종양 중 양성 자궁 평활근종 21예, 심프라스틱(symplastic) 자궁 평활근종 2예, 이형성(atypical) 자궁평활근종 1예, 경계형(borderline) 자궁 평활근종 1예와 악성 평활근종 3예를 대상으로 이들의 파라핀 포매된 조직중 대표적인 것을 선정하여 유세포 측정을 시행하고 그 분열상에 있어서 어떠한 특징이 있는지 비교 검토한 결과, 21예의 양성 자궁 평활근종중 단지 2예와 3예의 악성 자궁 평활근종중 1예에서만 이수배수체(aneuploidy) 양상을 보였고, 다른 모든 예에서는 이배체(diploidy)를 보였다. 히스토그램 분석상, 양성 자궁 평활근종과 심프라스틱, 이형성, 경계형 자궁 평활근종과 악성 평활근종의 G_(2)+M/G_(1)비에서 유의한 차이를 보여 주었으며(P<0.05), G_(2)+M/G_(1)비에서는 양성과 양성이 아닌 군사이에서 아주 유의한 차이를 보여 주었다(P<0.001). 그러나 배수성 양상, G_(0)/G_(1), S, G_(2)/M, 증식지수(proliferation index)와 DNA지수는 어느 군사이에서도 차이를 보여주지 않았다. 양성종양(leiomyoma) 및 악성종양(leiomyosarcoma)의 이배체군과 이수배수체군들 사이의 각각의 비교에서 이배체군의 G_(2)+M/G_(1)비에서 유의한 차이를 보여주었고, 이수배수체군에서 G_(0)/G_(1)비에서 다소의 차이를 보여 주었으나 통계학적으로 유의하지는 않았다. Flow cytometric DNA content analysis of 21 cases of benign leiomyomas, 2 cases of symplastic leiomyomas, 1 case of atypical leiomyoma, 1 case of borderline malignancy of leiomyoma and 3 cases of leiomyosarcomas with paraffin blocks from uterine hysterectomy specimen were performed. Only two cases out of 21 cases of benign leiomyomas (9%) showed aneuploid patterns, one out of three leiomyosarcomas was aneuploidy and all others were diploidy. In histograms, the only G_(2)+M/G_(1) ratio fraction showed significant differences between benign leiomyomas and other symplastic, atypical leiomyomas, borderline malignancy of leiomyoma and leiomyosarcomas(P<0.05). In histogramic fractions between benign and non-benign tumors, there were very significants differences in G_(0)+M/G_(1) ratio(P<0.001). There were no differences in ploidy pattern, G_(0)/G_(1) fraction, S phase fraction, G_(2)+M fraction, proliferation index and DNA index. Between benign leiomyomas and leiomyosarcomas, in cases of diploid tumors, only G_(2)+M/G_(1) ratio showed significant difference (P<0.05) and in cases of aneuploid tumors, there was only suggestion of difference in only G_(0)/G_(1) fraction.

New Orthoquinones from the Roots of Salvia lanigera

(Ik Soo Lee),(Norito Kaneda),(Rutt Suttisri),(Abdalla M . El-Lakany),(Nawal N . Sabri),(A . Douglas Kinghorn) 전남대학교 약품개발연구소 1998 약품개발연구지 Vol.7 No.1

N/A N/A

DNA Vaccine Encoding HCV Nonstructural Proteins Enhances Virus-Specific Cellular Immune Responses in Patients with Chronic Hepatitis C

( Ji Won Han ),( Pil Soo Sung ),( Seon-hui Hong ),( Hyojin Lee ),( Moonsup Jeong ),( Su-hyung Park ),( Jian Yan ),( Amir Khan ),( Joel N. Maslow ),( David B. Weiner ),( Jeong Heo ),( Sang Hoon Ahn ),( 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Although direct-acting antivirals (DAA) are successfully used for the treatment of chronic hepatitis C, there are DAA-resistant cases. Furthermore, DAA-treated patients do not develop protective immunity against HCV re-infection. To complement DAA therapy, we developed a DNA vaccine (VGX- 6150) containing three plasmids encoding HCV genotype 1 NS3, NS4 and NS5A consensus immunogens and plasmid encoding IL-28B as a molecular adjuvant. In the present study, we performed a phase I clinical trial of VGX-6150 in patients with chronic hepatitis C (n=18) who had failed interferon (IFN)-based treatment. Methods: VGX-6150 was administered 4 times in 4-week intervals with in vivo electroporation to three groups of patients (n=6 for 1, 3, and 6 mg/dose groups, respectively) followed by a 6 mg boost 24 weeks later. Significant side effects were not reported. Cellular immune responses of peripheral blood mononuclear cells (PBMCs) were evaluated by IFN-g ELISpot assays using overlapping peptides spanning NS3~NS5A and staining with HCV-specific HLA class I pentamers. Results: The IFN-g spot number specific to NS3~NS5A was increased in 3 and 6 mg/dose groups after the initial vaccination and in all three groups after the boosting vaccination. However, the frequency of HCV-specific HLA class I pentamer+ CD8+ T-cells was not increased by the vaccination. Moreover, the frequency of PD-1+ cells in the gate of HLA class I pentamer+ CD8+ T-cells was not changed by the vaccination. Interestingly, the frequency of CD4+CD25+Foxp3+ regulatory T-cells (Treg) was significantly decreased after the vaccination, consistent with a previous report regarding the effect of an IL-28B-encoding plasmid adjuvant. Conclusions: In the present study, we demonstrate that VGX- 6150 enhances HCV antigen-specific T-cell responses without significant side effects. The inclusion of IL-28B as an immune adjuvant was associated with a decrease in Treg cells that may have provided greater immune activation in chronic HCV infec-tion.

Overall Prevalence and Distribution of Knockdown Resistance (kdr) Mutations in Aedes aegypti from Mandalay Region, Myanmar

Haung Naw,Mya Nilar Chaw Su,Tu?n C??ng Vo,H??ng Giang Le,Jung-Mi Kang,Hojong Jun,Yi Yi Mya,Moe Kyaw Myint,Jinyoung Lee,Woon-Mok Sohn,Tong-Soo Kim,Byoung-Kuk Na 대한기생충학열대의학회 2020 The Korean Journal of Parasitology Vol.58 No.6

Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) of mosquitoes confer resistance to insecticides. Although insecticide resistance has been suspected to be widespread in the natural population of Aedes aegypti in Myanmar, only limited information is currently available. The overall prevalence and distribution of kdr mutations was analyzed in Ae. aegypti from Mandalay areas, Myanmar. Sequence analysis of the VGSC in Ae. aegypti from Myanmar revealed amino acid mutations at 13 and 11 positions in domains II and III of VGSC, respectively. High frequencies of S989P (68.6%), V1016G (73.5%), and F1534C (40.1%) were found in domains II and III. T1520I was also found, but the frequency was low (8.1%). The frequency of S989P/V1016G was high (55.0%), and the frequencies of V1016G/F1534C and S989P/V1016G/F1534C were also high at 30.1% and 23.5%, respectively. Novel mutations in domain II (L963Q, M976I, V977A, M994T, L995F, V996M/A, D998N, V999A, N1013D, and F1020S) and domain III (K1514R, Y1523H, V1529A, F1534L, F1537S, V1546A, F1551S, G1581D, and K1584R) were also identified. These results collectively suggest that high frequencies of kdr mutations were identified in Myanmar Ae. aegypti, indicating a high level of insecticide resistance.

A multicenter, randomized, and double-blind phase IV clinical trial to compare the efficacy and safety of fixed-dose combinations of amlodipine orotate/valsartan 5/160 mg versus valsartan/hydrochlorothiazide 160/12.5 mg in patients with ess

Ahn, Youngkeun,Kim, Yongcheol,Chang, Kiyuk,Kim, Weon,Rhee, Moo-Yong,Cha, Kwang Soo,Hyon, Min Su,Shim, Chi Young,Lee, Sung Yun,Kim, Doo Il,Kim, Sang Wook,Lim, Sang-Wook,Han, Kyoo-Rok,Jo, Sang-Ho,Lee, N Williams & Wilkins Co 2018 Medicine Vol.97 No.37

<P><B>Abstract</B></P><P><B>Background:</B></P><P>To determine whether the effectiveness and safety of fixed-dose combinations (FDCs) of amlodipine orotate/valsartan (AML/VAL) 5/160 mg are noninferior to those of valsartan/hydrochlorothiazide (VAL/HCTZ) 160/12.5 mg in hypertensive patients with inadequate response to valsartan 160 mg monotherapy.</P><P><B>Methods:</B></P><P>This 8-week, active-controlled, parallel-group, fixed-dose, multicenter, double-blind randomized controlled, and noninferiority trial was conducted at 17 cardiovascular centers in the Republic of Korea. Eligible patients had mean sitting diastolic blood pressure (msDBP) ≥90 mm Hg despite monotherapy with valsartan 160 mg for 4 weeks. Patients were randomly assigned to treatment with AML/VAL 5/160 mg FDC (AML/VAL) group or VAL/HCTZ 160/12.5 mg FDC (VAL/HCTZ) group once daily for 8 weeks. A total of 238 patients were enrolled (AML/VAL group, n = 121; VAL/HCTZ group, n = 117), of whom 228 completed the study.</P><P><B>Results:</B></P><P>At 8 weeks after randomization, msDBP was significantly decreased in both groups (−9.44 ± 0.69 mm Hg in the AML/VAL group and −7.47 ± 0.71 mm Hg in the VAL/HCTZ group, both <I>P</I> < .001 vs baseline). Between group difference was −1.96 ± 1.00 mm Hg, indicating that AML/VAL 5/160 mg FDC was not inferior to VAL/HCTZ 160/12.5 mg FDC at primary efficacy endpoint. Control rate of BP defined as the percentage of patients achieving mean sitting SBP (msSBP) <140 mm Hg or msDBP <90 mm Hg (target BP) from baseline to week 8 was significantly higher in the AML/VAL group than that in the VAL/HCTZ group (84.3% [n = 102] in the AML/VAL group vs 71.3% [n = 82] in the VAL/HCTZ group, <I>P</I> = .016). At 8 weeks after randomization, mean uric acid level was significantly increased in the VAL/HCTZ group compared to that at baseline (0.64 ± 0.08 mg/dL; <I>P</I> < .001). However, it was slightly decreased from baseline in the AML/VAL group (−0.12 ± 0.08 mg/dL; <I>P</I> = .085). The intergroup difference was significant (<I>P</I> < .001).</P><P><B>Conclusion:</B></P><P>The effectiveness and safety AML/VAL 5/160 mg FDC are noninferior to those of VAL/HCTZ 160/12.5 mg FDC in patients with hypertension inadequately controlled by valsartan 160 mg monotherapy.</P>

[논문]천연염색에 관한 연구 -자근에 의한 면의 염색에서 농색화와 세탁견뢰도 향상-

김혜인,최해욱,박수민 釜山大學校生産技術硏究所 2008 生産技術硏究所論文集 Vol.67 No.-

천연의 금속매염염료인 자근에 의한 변의 염색에서 농후,견뢰염색을 목적으로 각종의 염색조건의 변화 및 변의 개질에 따른 염료의 염색성을 비교 ? 검토하였다. 그 결과 자근색소추출은 아세톤추출이 가장 적당하였으며,온도와 pH가 높을수록 염색시료가 bluish색상을 나타내었다. 카르복시메틸화면에 선매염후 67"C 에서의 염색이 염착량 증대 및 세탁견뢰도 향상에 효과적이었다.

Retrogradation of Dilute Starch Dispersion

N am-Soo Kim(김남수),Young-Jung Nam(남영중),Dong-Hwa Shin(신동화) 한국식품과학회 1988 한국식품과학회지 Vol.20 No.1

크랙과 이동질량을 가진 유체듀동 단순지지 파이프의 동특성에 관한 연구

손인수(]n-Soo Son),안성진(Sung-Jin Ahn),윤한익(Han-Ik Yoon) 대한기계학회 2003 대한기계학회 춘추학술대회 Vol.2003 No.11

An iterative modal analysis approach is developed to determine the effect of the transverse open cracks and the moving mass on the dynamic behavior of simply supported pipe conveying fluid. The equation of motion is derived by using Lagrange's equation. The influences of the velocity of moving mass, the velocity of fluid flow and a crack have been studied on the dynamic behavior of a simply supported pipe system by numerical method. The crock section is represented by u local flexibility matrix connecting two undamaged beam segments. that is, the crack is modelled as a rotational spring. Totally, 0:; the velocity of fluid flow is increased, the mid-spun deflection of simply supported pipe conveying fluid is increased. The position of the crack is middle point of the pipe, the mid-span deflection of simply supported pipe presents maximum deflection.