Knockdown of lncRNA PVT1 Inhibits Vascular Smooth Muscle Cell Apoptosis and Extracellular Matrix Disruption in a Murine Abdominal Aortic Aneurysm Model

Zhidong Zhang,Gangqiang Zou,Xiaosan Chen,Wei Lu,Jianyang Liu,Shuiting Zhai,Gang Qiao 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.3

This study was designed to determine the effects of the long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) on vascular smooth muscle cell (VSMC) apoptosis and extracellular matrix (ECM) disruption in a murine abdominal aortic aneurysm (AAA) model. After injection of PVT1-silencing lentiviruses, AAA was induced in Apolipoprotein E-deficient (ApoE?/?) male mice by angiotensin II (Ang II) infusion for four weeks. After Ang II infusion, mouse serum levels of pro-inflammatory cytokines were analysed, and aortic tissues were isolated for histological, RNA, and protein analysis. Our results also showed that PVT1 expression was significantly upregulated in abdominal aortic tissues from AAA patients compared with that in controls. Additionally, Ang II treatment significantly increased PVT1 expression, both in cultured mouse VSMCs and in AAA murine abdominal aortic tissues. Of note, the effects of Ang II in facilitating cell apoptosis, increasing matrix metalloproteinase (MMP)-2 and MMP-9, reducing tissue inhibitor of MMP (TIMP)-1, and promoting switching from the contractile to synthetic phenotype in cultured VSMCs were enhanced by overexpression of PVT1 but attenuated by knockdown of PVT1. Furthermore, knockdown of PVT1 reversed Ang II-induced AAA-associated alterations in mice, as evidenced by attenuation of aortic diameter dilation, marked adventitial thickening, loss of elastin in the aorta, enhanced aortic cell apoptosis, elevated MMP-2 and MMP-9, reduced TIMP-1, and increased proinflammatory cytokines. In conclusion, our findings demonstrate that knockdown of lncRNA PVT1 suppresses VSMC apoptosis, ECM disruption, and serum pro-inflammatory cytokines in a murine Ang II-induced AAA model.

CATALYTIC DECOMPOSITION OF ο-DICHLOROBENZENE IN AIR OVER TiO2 - BASED POWDER CATALYSTS

Zhidong Jiang,Bong Soek Gil,Chang Mo Nam,Jong Shik Chung 대한환경공학회 2000 Environmental Engineering Research Vol.5 No.4

Constant Envelope Enhanced FQPSK and Its Performance Analysis

Zhidong Xie,Gengxin Zhang,Dongming Bian 한국통신학회 2011 Journal of communications and networks Vol.13 No.5

It’s a challenging task to design a high performance modulation for satellite and space communications due to the limited power and bandwidth resource. Constant envelope modulation is an attractive scheme to be used in such cases for their needlessness of input power back-off about 2∼3 dB for avoidance of nonlinear distortion induced by high power amplifier. The envelope of Feher quadrature phase shift keying (FQPSK) has a least fluctuation of 0.18 dB (quasi constant envelope) and can be further improved. This paper improves FQPSK by defining a set of new waveform functions, which changes FQPSK to be a strictly constant envelope modulation. The performance of the FQPSK adopting new waveform is justified by analysis and simulation. The study results show that the novel FQPSK is immune to the impact of HPA and outperforms conventional FQPSK on bit error rate (BER) performance. The BER performance of this novel modulation is better than that of FQPSK by more than 0.5 dB at least and 2 dB at most.

Study on Adaptation of Blackcurrant to Soil Ph Value

Zhidong Zhang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

What Kind of Nation-State Do They Want to Build: Chinese Intellectuals and Nationalism in Contemporary China

Zhidong Hao 글로벌지식융합학회 2021 글로벌지식융합학회 학술대회 자료집 Vol.2021 No.6

Distributed Filtering for Markovian Jump Systems With Packet Loss Compensation

Zhidong Zhou,Yuyan Wu,Jun Cheng,Yunliang Wang 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.7

In this article, the distributed-based filtering problem is studied for a type of Markov jump system subject to mode-dependent random packet losses. Packet dropout happens during the communication channel, which can be described via a random variable. In order to describe the nonhomogeneous Markov process, transition probabilities (TPs) on polytopic-structure-based are introduced. In view of packet loss, added by the single exponential smoothing (SES) technique, the packet loss compensation is provided to deal with the prediction value of the missing output. Besides, as for the physical limitations, it is quite difficult to ensure that all mode signals of the plant are available to filter. In the sequel, we establish a partially mode-dependent filter (PMDF). By using the Lyapunov theory, some sufficient conditions are obtained to guarantee the stochastically stable (SS) of the filtering error system (FES), and the filter parameters can be derived. Lastly, the validity of the presented results is tested through a numerical example.

Seismic mitigation of an existing building by connecting to a base-isolated building with visco-elastic dampers

Zhidong Yang,Eddie S.S. Lam 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.1

This study investigates the feasibility of retrofitting an existing building by connecting theexisting building to a new building using connecting dampers. The new building is base-isolated and viscoelastic dampers are assigned as connecting dampers. Scaled models are tested under three different earthquake records using a shaking table. The existing building and the new building are 9 and 8 storiesrespectively. The existing building model shows more than 3% increase in damping ratio. The maximum dynamic responses and the root mean square responses of the existing building model to earthquakes are substantially reduced by at least 20% and 59% respectively. Further, numerical models are developed by conducting time-history analysis to predict the performance of the proposed seismic mitigation system. The predictions agree well with the test results. Numerical simulations are carried out to optimize the properties of connecting dampers and base isolators. It is demonstrated that more than 50% of the peak responses canbe reduced by properly adjusting the properties of connecting dampers and base isolators.

Constant Envelope Enhanced FQPSK and Its Performance Analysis

Xie, Zhidong,Zhang, Gengxin,Bian, Dongming The Korea Institute of Information and Commucation 2011 Journal of communications and networks Vol.13 No.5

It's a challenging task to design a high performance modulation for satellite and space communications due to the limited power and bandwidth resource. Constant envelope modulation is an attractive scheme to be used in such cases for their needlessness of input power back-off about 2~3 dB for avoidance of nonlinear distortion induced by high power amplifier. The envelope of Feher quadrature phase shift keying (FQPSK) has a least fluctuation of 0.18 dB (quasi constant envelope) and can be further improved. This paper improves FQPSK by defining a set of new waveform functions, which changes FQPSK to be a strictly constant envelope modulation. The performance of the FQPSK adopting new waveform is justified by analysis and simulation. The study results show that the novel FQPSK is immune to the impact of HPA and outperforms conventional FQPSK on bit error rate (BER) performance. The BER performance of this novel modulation is better than that of FQPSK by more than 0.5 dB at least and 2 dB at most.

Knockdown of lncRNA PVT1 Inhibits Vascular Smooth Muscle Cell Apoptosis and Extracellular Matrix Disruption in a Murine Abdominal Aortic Aneurysm Model

Zhang, Zhidong,Zou, Gangqiang,Chen, Xiaosan,Lu, Wei,Liu, Jianyang,Zhai, Shuiting,Qiao, Gang Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.3

This study was designed to determine the effects of the long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) on vascular smooth muscle cell (VSMC) apoptosis and extracellular matrix (ECM) disruption in a murine abdominal aortic aneurysm (AAA) model. After injection of PVT1-silencing lentiviruses, AAA was induced in Apolipoprotein E-deficient ($ApoE^{-/-}$) male mice by angiotensin II (Ang II) infusion for four weeks. After Ang II infusion, mouse serum levels of pro-inflammatory cytokines were analysed, and aortic tissues were isolated for histological, RNA, and protein analysis. Our results also showed that PVT1 expression was significantly upregulated in abdominal aortic tissues from AAA patients compared with that in controls. Additionally, Ang II treatment significantly increased PVT1 expression, both in cultured mouse VSMCs and in AAA murine abdominal aortic tissues. Of note, the effects of Ang II in facilitating cell apoptosis, increasing matrix metalloproteinase (MMP)-2 and MMP-9, reducing tissue inhibitor of MMP (TIMP)-1, and promoting switching from the contractile to synthetic phenotype in cultured VSMCs were enhanced by overexpression of PVT1 but attenuated by knockdown of PVT1. Furthermore, knockdown of PVT1 reversed Ang II-induced AAA-associated alterations in mice, as evidenced by attenuation of aortic diameter dilation, marked adventitial thickening, loss of elastin in the aorta, enhanced aortic cell apoptosis, elevated MMP-2 and MMP-9, reduced TIMP-1, and increased pro-inflammatory cytokines. In conclusion, our findings demonstrate that knockdown of lncRNA PVT1 suppresses VSMC apoptosis, ECM disruption, and serum pro-inflammatory cytokines in a murine Ang II-induced AAA model.

Seismic mitigation of an existing building by connecting to a base-isolated building with visco-elastic dampers

Yang, Zhidong,Lam, Eddie S.S. Techno-Press 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.1

This study investigates the feasibility of retrofitting an existing building by connecting the existing building to a new building using connecting dampers. The new building is base-isolated and viscoelastic dampers are assigned as connecting dampers. Scaled models are tested under three different earthquake records using a shaking table. The existing building and the new building are 9 and 8 stories respectively. The existing building model shows more than 3% increase in damping ratio. The maximum dynamic responses and the root mean square responses of the existing building model to earthquakes are substantially reduced by at least 20% and 59% respectively. Further, numerical models are developed by conducting time-history analysis to predict the performance of the proposed seismic mitigation system. The predictions agree well with the test results. Numerical simulations are carried out to optimize the properties of connecting dampers and base isolators. It is demonstrated that more than 50% of the peak responses can be reduced by properly adjusting the properties of connecting dampers and base isolators.