Gene Expression Profiles of HeLa Cells Impacted by Hepatitis C Virus Non-structural Protein NS4B

Zheng, Yi,Ye, Lin-Bai,Liu, Jing,Jing, Wei,Timani, Khalid A.,Yang, Xiao-Jun,Yang, Fan,Wang, Wei,Gao, Bo,Wu, Zhen-Hui Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.2

By a cDNA array representing 2308 signal transduction related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RT-PCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeletion and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.

Centromere protein N promotes lung adenocarcinoma progression by activating PI3K/AKT signaling pathway

Zheng Yi,You Hui,Duan Jingzhu,Chen Biyu,Wu Chenlin,Chen Peipei,Wang Meifang 한국유전학회 2022 Genes & Genomics Vol.44 No.9

Background: As an important member of centromere family, centromere associated protein N (CENPN) was abnormally expressed in varied malignant tumors. Objective: This paper aimed to analyze the expression and related mechanism of CENPN in lung adenocarcinoma (LUAD). Methods: The expression of CENPN in LUAD was analyzed by Gene Expression Profiling Interactive Analysis (GEPIA) database. The mRNA expression, protein expression, cell viability, cell invasion, cell apoptosis, cell stem like characteristics were detected by RT-PCR, western blot, CCK8 assay, transwell assay, flow cytometry and spheroidization assay, respectively. Finally, the pathological changes of xenograft were estimated by H&E staining, and the expression of proteins was detected by immunohistochemistry. Results: GEPIA analysis showed that the CENPN expression in LUAD was significantly higher than that in normal lung tissue, which was negatively correlated with the prognosis. These results were consistent with our clinical data. Besides, CENPN was highly expressed in LUAD cell lines. In addition, the upregulation of CENPN amplified the cell viability, stemness and invasive ability in PC9 cells. However, the knockdown of CENPN inhibited the cell activity, stemness, invasive ability with increased cell apoptosis in A549. Furthermore, CENPN could positively regulate the phosphorylation of PI3K and AKT. The PI3K inhibitor, 740Y-P, could reverse the effect of CENPN silencing on the expression of Ki-67, cleaved caspase 3, OCT4, and snail 1. Finally, the downregulation of CENPN restrained the growth of xenograft and inactivated the PI3K/AKT pathway. Conclusion: CENPN was abnormally overexpressed in LUAD, and promoted tumor progression of LUAD by affecting PI3K/AKT pathway.

Sampling artifact in volume weighted velocity measurement. II. Detection in simulations and comparison with theoretical modeling

Zheng, Yi,Zhang, Pengjie,Jing, Yipeng American Physical Society 2015 PHYSICAL REVIEW D - Vol.91 No.4

Solubility of D(−)-p-Hydroxyphenylglycine dane salt in binary methanol+isopropanol solvent mixtures

Zheng-Ming Yi,Qun-Sheng Li,Wei-Zhong Liu,Xu-Feng Sun 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.3

The solubility of D(−)-p-Hydroxyphenylglycine Dane Salt (HPGDane Salt) in binary methanol and isopropanol solvent mixtures was measured using a laser technique with the temperature range from 283.15 K to 323.15 K and mole fraction (x2) range from 0.0000 to 1.0000. The results were correlated with a semi-empirical equation.

기억 내러티브의 이중성과 역사화의 탈정치성

yi Zheng,전성곤 경희대학교(국제캠퍼스) 국제지역연구원 2016 아태연구 Vol.23 No.4

In the Japanese earthquake this argument through narrative analysis of the Parties to the disaster experience. And then how to presents what is associated with pre-disaster recovery development of the dictionary. To this end, the first chapter in history and reviewed the relevance of the narrative. Especially individuals who can not fall of experience in the flow of history and review historical revisionism methodology that mean to the excitement about memory. It was a memory for ham to the ‘process’ of representation Post-narrative voice’ the history of the problem. Indicate this is conscious of hot new and memories of past experiences in the narrative in chapter 4 cover ‘substance’ offered logic that must free ourselves from memory. It was a national standpoint, and institutional sharing of historical ‘awareness of transplant’ that is not taken into consideration in order to deploying. Is a disaster memory of awareness by ‘human tolerance’ that was made by narrative. 본 논고에서는 동일본대지진 경험자인 당사자 내러티브 분석을 통해, 재해의 경험이나 기억이 어떤 의미에서 역사화되는가에 대한 문제를 고찰한다. 이를 위해 제1장에서는 역사와 내러티브의 관련성에 대해 다루어본다. 특히 역사학의 흐름에서 빠질 수 없는 개인의 경험과 기억에 대한 역사수정주의 방법론의 등장이 갖는 의미를 검토했다. 또한 내러티브(narrative)가 ‘현재적 의미에서 과거를 재현하는 것’이라는 의미를 갖는 역사성을 살펴보았다. 제2장에서는 사실로서 존재했던 ‘동일본대지진’과 ‘동일본대지진 내러티브’가 사회적 패러다임으로 자리를 잡아가는 논리의 문제점을 검토했다. 제3장에서는 내러티브 속에서 나타나는 기억의 재현이 갖는 한계점과 가능성을 고찰했다. 즉, 기억의 재현이라는 것이 사물들에 의해 지배되는 것이 아니라, 새롭게 자아를 발견하는 것임을 제시했다. 마지막으로는 새로운 내러티브 텍스트가 발생되어 화자와 청취자의 내부에서 공동으로 발견되는 ‘상호적 자아 내부의 타자성’이라는 제3의 인식이 창출될 가능성을 고찰했다.

Interconnected hollow carbon nanospheres for stable lithium metal anodes

Zheng, Guangyuan,Lee, Seok Woo,Liang, Zheng,Lee, Hyun-Wook,Yan, Kai,Yao, Hongbin,Wang, Haotian,Li, Weiyang,Chu, Steven,Cui, Yi Nature Publishing Group, a division of Macmillan P 2014 Nature nanotechnology Vol.9 No.8

For future applications in portable electronics, electric vehicles and grid storage, batteries with higher energy storage density than existing lithium ion batteries need to be developed. Recent efforts in this direction have focused on high-capacity electrode materials such as lithium metal, silicon and tin as anodes, and sulphur and oxygen as cathodes. Lithium metal would be the optimal choice as an anode material, because it has the highest specific capacity (3,860 mAh g<SUP>–1</SUP>) and the lowest anode potential of all. However, the lithium anode forms dendritic and mossy metal deposits, leading to serious safety concerns and low Coulombic efficiency during charge/discharge cycles. Although advanced characterization techniques have helped shed light on the lithium growth process, effective strategies to improve lithium metal anode cycling remain elusive. Here, we show that coating the lithium metal anode with a monolayer of interconnected amorphous hollow carbon nanospheres helps isolate the lithium metal depositions and facilitates the formation of a stable solid electrolyte interphase. We show that lithium dendrites do not form up to a practical current density of 1 mA cm<SUP>–2</SUP>. The Coulombic efficiency improves to ∼99% for more than 150 cycles. This is significantly better than the bare unmodified samples, which usually show rapid Coulombic efficiency decay in fewer than 100 cycles. Our results indicate that nanoscale interfacial engineering could be a promising strategy to tackle the intrinsic problems of lithium metal anodes.

Software safety in the evolution of autonomous cars

Yi Zheng 한국자동차공학회 2014 한국자동차공학회 Workshop Vol.2014 No.10

Enhanced Photocatalytic Performance of Heterojunction BiOI/BiOIO3 Nanocomposites Under Simulated Solar Light

Yi Ling Qi,Xu Chun Song,Yi Fan Zheng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.3

Novel heterostructure BiOI/BiOIO3 nanocomposites were successfully prepared through a facile deposition method at room temperature. BiOIO3 is a noncentrosymmetric compound that has an internal self-built electric field. BiOI was applied as a visible light absorber to sensitize semiconductors owing to its smallest bandgap. The coupling between BiOIO3 and BiOI can combine their advantages and improve photocatalytic properties. Compared with the single BiOI and BiOIO3, the heterostructure BiOI/BiOIO3 nanocomposites displayed a significantly enhanced photocatalytic activity for the Rhodamine B (RhB) degradation. The enhanced photocatalytic performance is deduced closely related to the formation of BiOI/BiOIO3 heterojunction interface whose presence is regarded to be a favorable factor for the transfer and separation of the photogenerated electrons and holes.

Hepatitis C Virus Non-structural Protein NS4B Can Modulate an Unfolded Protein Response

Yi Zheng,Bo Gao,Li Ye,Lingbao Kong,Wei Jing,Xiaojun Yang,Zhenghui Wu,Linbai Ye 한국미생물학회 2005 The journal of microbiology Vol.43 No.6

Viral infection causes stress to the endoplasmic reticulum (ER). The response to endoplasmic reticulum stress, known as the unfolded protein response (UPR), is designed to eliminate misfolded proteins and allow the cell to recover. The role of hepatitis C virus (HCV) non-structural protein NS4B, a component of the HCV replicons that induce UPR, is incompletely understood. We demonstrate that HCV NS4B could induce activating transcription factor (ATF6) and inositol-requiring enzyme 1 (IRE1), to favor the HCV subreplicon and HCV viral replication. HCV NS4B activated the IRE1 pathway, as indicated by splicing of X box-binding protein (Xbp-1) mRNA. However, transcriptional activation of the XBP-1 target gene, EDEM (ER degradation-enhancing α-mannosidase-like protein, a protein degradation factor), was inhibited. These results imply that NS4B might induce UPR through ATF6 and IRE1- XBP1 pathways, but might also modify the outcome to benefit HCV or HCV subreplicon replication.

Study on mapping of dark matter clustering from real space to redshift space

Yi Zheng,Yong-Seon Song 한국천문학회 2016 天文學會報 Vol.41 No.1