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( Zongsheng Zou ),( Yunying Zhao ),( Tingzhou Zhang ),( Jiaxing Xu ),( Aiyong He ),( Yu Deng ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.9
A cellulase hyperproducing mutant strain, JNDY-13, was obtained using the ARTP mutation system and with Trichoderma reesei RUT-C30 as the parent strain. Whole-genome sequencing of JNDY-13 confirmed that 105 of the 653 SNPs were point mutations, 336 mutations were deletions and 165 were insertions. Moreover, 99 mutations were insertions and duplications. Among all the mutations, the one that occurred in the galactokinase gene might be related to the production of cellulases in T. reesei JNDY-13. Moreover, the up-regulation of cellulase and hemicellulase genes in JNDY-13 might contribute to higher cellulases production. Under optimal conditions, the highest cellulase activity by batch fermentation reached 4.35 U/ml, and the highest activity of fed-batch fermentation achieved was 5.40 U/ml.
Xianglan Kong,Xinhua Ma,Yunying Xie,Shengxin Cai,Tianjiao Zhu,Qian Qun Gu,De-Hai Li 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.6
In our screening for antitubercular agents, fivenaphtho-c-pyrones including two new naphtho-c-pyronesglycosides, indigotides G and H (1 and 2), and two diphenylethers were isolated from the extract of a sponge-derivedfungus Metarhizium anisopliae mxh-99. Their structureswere established on the basis of chemical and spectroscopicevidence. The antitubercular activities of all the compoundswere evaluated against Mycobacterium phlei. The knownisochaetochromin B2 (6) and ustilaginoidin D (7) exhibitedthe highest activity with MICs 50.0 lg/mL.
Phosphorus Release from Lake Sediments: Effects of pH,Temperature and Dissolved Oxygen
YunHai Wu,Yajun Wen,Jianxin Zhou,Yunying Wu 대한토목학회 2014 KSCE JOURNAL OF CIVIL ENGINEERING Vol.18 No.1
Sediments can release Phosphorus (P) into overlying water under certain environmental conditions, which may have a significant impact on water quality and result in continuous eutrophication. We used laboratory experiments to evaluate effects of pH,temperature, and Dissolved Oxygen (DO) on P release from Lake Xuanwu, China. The results demonstrate that effects of pH on P release from sediments were distinct; more P was released under alkaline conditions than acidic conditions, but the least amount of P was released under neutral pH. Increasing temperature resulted in more release of P and a longer time to achieve equilibrium between sediment P release and P in overlying water. Influences of DO supply levels on P release were also quite significant; anaerobic conditions were more conducive to the release of P than aerobic conditions. Orthogonal experiments show that the 3 significant factors on P release from sediments were, in descending order, DO, temperature, and pH. These findings have important management implications for Lake Xuanwu.
Lee, Su-Yeon,Kim, Jung Ran,Hu, Yunying,Khan, Raffay,Kim, Su-Jung,Bharadwaj, Kalyani G.,Davidson, Mercy M.,Choi, Cheol-Soo,Shin, Kyong-Oh,Lee, Yong-Moon,Park, Woo-Jin,Park, In-Sun,Jiang, Xian-Cheng,Gol American Society for Biochemistry and Molecular Bi 2012 The Journal of biological chemistry Vol.287 No.22
The Differences between Luminal Microbiota and Mucosal Microbiota in Mice
Minna Wu,Puze Li,Jianmin Li,Yunying An,Mingyong Wang,Genshen Zhong 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.2
The differences between luminal microbiota (LM) and mucosal microbiota (MAM) were little known, especially in duodenum. In this study, LM and MAM in colon and duodenum of mice were investigated through 16S rRNA high-throughput sequencing. The lowest bacterial diversity and evenness were observed in duodenal LM (D_LM), followed by duodenal MAM (D_MAM). Meanwhile, the bacterial diversity and evenness were obviously increased in D_MAM than these in D_LM, while no significant difference was observed between colonic MAM (C_MAM) and colonic LM (C_LM). PCoA analysis also showed that bacterial communities of LM and MAM in duodenum were completely separated, while these in colon overlapped partly. The ratio of Firmicutes to Bacteroidetes (F/B) in D_MAM was significantly higher than that in D_LM. Lactobacillus was largely enriched and was the characteristic bacteria in D_LM. The characteristic bacteria in D_MAM were Turicibacter, Parasutterella, Marvinbryantia and Bifidobacterium, while in C_LM they were Ruminiclostridium_6, Ruminiclostridium_9, Ruminococcaceae_UCG_007 and Lachnospiraceae_UCG_010, and in C_MAM they were Lachnospiraceae_NK4A136, Mucispirillum, Alistipes, Ruminiclostridium and Odoribacter. The networks showed that more interactions existed in colonic microbiota (24 nodes and 74 edges) than in duodenal microbiota (17 nodes and 29 edges). The 16S rDNA function prediction results indicated that bigger differences of function exist between LM and MAM in duodenum than these in colon. In conclusion, microbiota from intestinal luminal content and mucosa were different both in colon and in duodenum, and bacteria in colon interacted with each other much more closely than those in duodenum.
Biosynthesis of adipic acid in metabolically engineered Saccharomyces cerevisiae
Zhang Xi,Liu Yingli,Wang Jing,Zhao Yunying,Deng Yu 한국미생물학회 2020 The journal of microbiology Vol.58 No.12
Adipic Acid (AA) is a valued platform chemical compound, which can be used as a precursor of nylon-6,6. Due to the generation of an enormous amount of nitric oxide metabolites and the growing depletion of oil resources as a result of AA production from a mixture of cyclohexanol and cyclohexanone, the microbial methods for synthesizing AA have attracted significant attention. Of the several AA-producing pathways, the reverse adipate degradation pathway in Thermobifida fusca (Tfu RADP) is reported to be the most efficient, which has been confirmed in Escherichia coli. In this study, the heterologous Tfu RADP was constructed for producing AA in S. cerevisiae by co-expressing genes of Tfu_ 0875, Tfu_2399, Tfu_0067, Tfu_1647, Tfu_2576, and Tfu_ 2576. The AA titer combined with biomass, cofactors and other by-products was all determined after fermentation. During batch fermentation in a shake flask, the maximum AA titer was 3.83 mg/L, while the titer increased to 10.09 mg/L during fed-batch fermentation in a 5-L bioreactor after fermentation modification.