주정 중독 흰쥐에서 총담관 결찰이 간 및 혈청의 Glyoxalase I 활성에 미치는 영향

곽춘식,김여희,변용준 啓明大學校 醫科大學 1995 계명의대학술지 Vol.14 No.4

The activities of the rat liver and serum glyoxalase-I(GLO-I) were studied in cholestasis induced by common bile duct(CBD) ligation after chronic ethanol intoxication, and in cholestasis before acute ethanol intoxication to establish the biochemical background of alcohol hazards in hepatobiliary disease. GLO-I activities in the rat's liver and serum showed less increases when CBD was ligated after chronic ethanol intoxication than that in the CBD ligation alone. GLO-I activities in the rat's liver and serum showed no more increases when acute ethanol intoxication was induced after CBD ligation than that in the CBD ligation alone. On the other hand, when CBD was ligated after chronic ethanol intoxication, the value of Vmax of the liver GLO-I decreased significantly than that in the CBD was ligated alone. However, the values of Km of the liver GLO-I did not changes in the all experimental groups. Viewed from above results, when chronic ethanol intoxication is combined with cholestasis, GLO-I in the liver seems to be decrease its activity than that in cholestasis, and the cause of the decrease is thought to be decreased biosynthesis.

Enamel matrix derivative(EMDOGAIN^(R))을 이용한 상악 소구치의 자가이식 치험례

오유향,김효식,정현구,조태식,위유민,이창섭 조선대학교 구강생물학연구소 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.2

The success of tooth transplantation or replantation depends on the viability of periodontal ligament in the transplanted tooth. Mechanical injury to periodontal tissues frequently results in dental root resorption and ankylosis, which leads to the failure of transplantation or replantation. Enamel matrix derivative (EMD) has been recently used to induce periodontal regeneration. In this report, we show a clinical case of EMD application in the transplantation of an inversely impacted and immature tooth. An impacted second premolar was found in the right maxilla of a 14 year-old girl. The tooth was inversely impacted and the root formation was incomplete. When transplantation was carried out. EMD was applied to the periodontal tissues of the extracted premolar. The tooth was fixed at the correct position and the clinical condition was followed for evaluation for 3 months. Radiographs after 3 months exhibited new bone formation surrounding the transplanted tooth. There were no signs of root resorption or ankylosis, and reconstruction of the bone defect area at the maxilla was also found. Short-term results from this case indicated that EMD application was effective in the transplantation of an inversely impacted and immature tooth. In adddition, EMD might contribute to obtain good healing without ankylosis. Further follow-up observations are necessary to evaluate the regenerative effects of EMD.

Effect of Acute Ethanol Intoxication on Hepatic Rhodanese Activity in Rats with Extrahepatic Cholestasis

곽춘식,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.2

Liver and serum rhodanese activities were determined in acute ethanol intoxicated rats with extrahepatic cholestasis induced by common bile duct ligation (CBD) to manifest the biochemical background of alcohol drinking hazard under the hepatobiliary disease. Liver cytosolic and microsomal rhodanese activities and these Vmax values in CBD ligated rats with acute ethanol intoxication were found to be decreased much more than that in CBD ligation alone. However, the difference of Km value on above hepatic enzyme was not found between the experimental groups. On the other hand, serum rhodanese activity in CBD ligated rats with acute ethanol intoxication was greater increased more than that in CBD ligation alone. These results indicate that the biosynthesis of the hepatic rhodanese decreases and the serum rhodanese activity increases in cholestasis combined with acute ethanol intoxication. reflecting damage of aggravated hapatocytic membrane. Accordingly, the resulting data supported the fact that alcoholic drinks were enzymologically harmful to the hepatobiliary disease.

저압 증발하의 열교환 특성에 관한 연구

신유식,이윤환,서종수,전영흥,정효민,정한식 경상대학교 해양산업연구소 2002 해양산업연구소보 Vol.15 No.-

The purpose of this research is to obtain the characteristics of heat transfer or heat exchange under the vacuum pressure. The general heat transfer or heat exchange can be changed with the experimental environment. In this report, the exothermic calorie and endothermic calorie were measured under the vacuum chamber. These two kinds of calories show the evaporative and condensing sides, respectively, and these calories were measured by using the parameter with flow rate.

흰쥐 재생간의 Malate Dehydrogenase의 활성치

김여희,곽춘식,문교철 啓明大學校 醫科大學 1986 계명의대학술지 Vol.5 No.2

A study was made on the changes in the activities of the following during 6 post-perative days: Cytosolic and mitochondrial malate dehydrogenase of regenerating rat livers, and rat serum malate dehydrogenase after 70% (median and left lateral lobes) partial hepatectomy. The activities of lactate dehydrogenase and alanine aminotransferase in both serum and cytosol of regenerating rat liver were also measured. After parial hepatectomy in the rats, activity of serum malate dehydrogenase trmendously increased in in the span between the 12 hours and the second day. The activity of cytoslic malate dehydrogenase in the regenerating liver significantly increased from the first to the second day after partial hepatectomy. However, no significant changes in hepatic mitochondrial malate dehydrogenase was noted throughout the experiments. The activities of serum lactate dehydrogenase and alanine aminotransferase markedly elevated after partial hepatectomy. And activity of alanine aminotransferase significantly decreased in the hepatic cytosol after partial hepatectomy but hepatic cytosolic lactate dehydrogenase showed no change.

흰쥐 재생간에서의 Aryl Sulfotransferase의 활성도

곽춘식,김여희,신미정 啓明大學校 醫科大學 1995 계명의대학술지 Vol.14 No.4

This study was made to know the changes of aryl sulfotransferase activity in regenerating rat liver. Cytosolic, mitochondrial and microsomal aryl sulfotransferase isozymes I,II and III, IV activities were determined in regenerating liver tissue following 70% (median and left lateral lobes) partial hepatectomy in rats for a period of ten days. The activities of these enzymes in serum were measured, the values of the Km and Vmax in these hepatic enzymes were also measured. The activities of cytosolic, Mitochondrial, and microsomal aryl sulfotransferase I,II in regenerating rat liver tissue showed a significant increase between the second and the third day, the first and the third day, and the twelfth hour and the third day, respectively, following partial hepatectomy. The activities of aryl sulfotransferase isozymes I,II and III,IV in rat serum showed a significant increase between the twelfth hour and the first day following partial hepatectomy. The values of Vmax of cytosolic, mitochndrial and icrosomal aryl sulfotransferase isozyme I,II and III,IV in the regenerating liver showed significant increases at the second day following partial hepatectomy However, the values of Km of the these hepatic enzymes did not change. In View of the above results, aryl sulfotransferase isozymes in regenerating rat liver suggest that these are the enzymes increasing their bisoyntheses in the regenerating stage.

한국 문학 영어권 번역 소개 연구 : 현황과 문제점 중심으로 Chiefly on present conditions and problems

李洧植 배화여자대학 2000 培花論叢 Vol.19 No.-

It is about 80 years since Korean literary works translated into English have been introduced to English-speaking countries. On a close examination, 169 literary works in total have so far been published in the English block. The purpose of this study based on these materials lies in examining the present conditions of translation and their realities, finding out problems through material analyses, and suggesting what major tasks of solving problems in translation are in future. The studies and discourses about introduction of Korean literature to foreign countries are not rarely to be seen. As a matter of fact, those are just the comprehensive approaches about translation into many foreign languages. Accordingly I dare to say that this study belongs to the first one in the light of full-scaled independent study focused only on the introduction of translated works to the English block.

Bi₂Sr₂Ca₁Cu₂O8+δ단결정의 전기저항과 전송메카니즘에 대한 연구

설정식,손인호,채건식,주유환 慶南大學校 附設 基礎科學硏究所 1998 硏究論文集 Vol.12 No.-

In this work, we have used Aslamasov-Larkin(AL) theory for analyzing the resistive and electrical mechanism of as-grown and 8 hour O₂annealed Bi₂Sr₂Ca₁Cu₂O8+δ samples. Tc is 89K for as-grown sample and 83K for annealed sample. By fluctuation conductivity using AL theory. we have examined that as-grown sample had 3D transition in the wide critical region than for the annealed sample.

Effects of Intravenous Administration of Taurocholate on Hepatic Aryl Sulfotransferase Activity in Cholestatic Rats

곽춘식,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1

The possible mechanisms of increased aryl sulfotransferase (AST) isozymes activities in cholestatic rat liver were studied. Hepatic AST-Ⅰ, Ⅱ and -Ⅲ, Ⅳ activities were determined from the experimental rats with common bile duct ligation (CBDL). The Michaelis-Menten constants in these hepatic enzymes were also measured. The activities of mitochondrial AST-Ⅰ, Ⅱ and -Ⅲ, Ⅳ, and microsomal AST-Ⅲ, Ⅳ as well as their Vmax values were found to be increased significantly in CBDL plus taurocholic acid (TCA) injected group than in the control group, such as CBDL alone groups. However, their Km values in the experimental groups did not vary. The results suggest that TCA stimulates biosynthesis of the AST in the liver.

Catechol-O-Methyltransferase Activity from Regenerating Liver after Partial Hepatectomy in Rats

곽춘식,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1

The change of catechol-O-methyltransferase (COMT) activity during regeneration of rat liver was studied. Cytosolic, mitochondrial and microsomal COMTs activities were estimated in regenerating rat livers over a period of ten days after 70% (median and left lateral lobes) partial hepatectomy. The values of Km and Vmax in the hepatic enzymes were also measured. The activities of cytosolic and microsomal COMTs in regenerating rat liver after partial hepatectomy were found to be significantly increased between the second and the third day. Whereas the mitochondrial COMT activity did not change. The Vmax values of the cytosolic and microsomal COMTs in the regenerating rat liver were significantly increased at the second day after partial hepatectomy, however, the Km values of the above hepatic enzymes did not vary in all the experimental groups. Therefore, the results suggest that the biosynthesis of COMT was increased during the regeneration of rat liver.