미립자 응집반응을 이용한 C-reactive Protein의 면역측정법에 관한 연구

최용경,정태화,최명자,김재화,최인성,김용호,송은영,이희구 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.1

환자의 복수와 늑막액으로부터 p-diazonium phenylphosphorylcholine(DPPC) coupled Separose-4B affinity chromatography와 hydroxylapatite chromatography를 실시하여 C-reactive protein (CRP)를 분리, 정제하였다. 정제된 CRP를 토끼에게 면역화하여 항혈청을 얻고 affinity chromatography를 하여 면역항체(IgG)를 분리하였다. 분리된 면역항체를 미립자에 감작시킨 후 미립자 응집반응에 의하여 3분내에 CRP를 측정할 수 있는 간이 면역측정법을 개발하였다. 본 연구에서 개발된 CRP측정법의 검출범위는 0.5∼20㎎/㎗이며, 임상 시험 결과 0.7∼2.9㎎/㎗에서는 강한 응집반응을, 5.0∼13.2㎎/㎗에서는 약한 응집반응을 보였고 28㎎/㎗이상에서는 항원 과잉으로 인한(zone of Ag excess phenomenon) 위음성을 나타냈다. 74명의 환자 혈청을 대상으로 CRP의 농도를 조사한 결과 평균치는 3.8㎎/㎗이었으며 대부분의 환자에서는 10㎎/㎗ 이하의 농도로 존재하였다. 그러므로 1차판정시 음성을 나타낸 시료라도 혈청을 5∼10배정도 희석하여 재분석한다면 오차없이 CRP를 검출할 수 있었다. 환자 혈청을 검체로 하여 본 연구에서 개발한 면역측정법과 현재 수입 시판중인 프랑스의 B사 제품과 일본의 I사 제품을 비교한 결과 좋은 상관관계를 보였다. 이와 같은 평가 분석을 통하여 볼 때 본 연구에서 개발한 간이 면역측정법은 사용이 비교적 간편하며 신빙성이 있어 CRP를 스크리닝 하는데 효과적임을 알 수 있었다. The C-reactive protein(CRP) from ascitic and pleural fluid was purified using calcium dependent affinity chromatography of CNBr activated Sepharose-4B covalently coupled to p-diazonium phenylphosphorylcholine(DPPC) and hydroxylapitite chromatography. Polyclonal antibody was prepared from rabbit by immunizing the purified CRP. Specific immunoglobulin G was isolated using affinity chromatography and coupled to microparticles. A sensitive microparticle-based immunoassay was developed to measure CRP within 3 mins. The detection range was between 0.5㎎/㎗ and 20㎎/㎗ in serum, showing strong response in the range of 0.7∼2.9㎎/㎗, week response in 5.0∼13.2㎎/㎗ and zone phenomenon over 28㎎/㎗. The average value of CRP in 74 samples was 3.8㎎/㎗ and most of the values were lower than 10㎎/㎗. The CRP values of serum samples were determined by our microparticle-based immunoassay, and were compared with those obtained using the other commercial products(B Co., France and I Co., Japan). Good correlations were shown between the values obtained by our developed microparticle-based immunoassay system and those by other commercial products. All performance characteristics evaluated make our developed microparticles-based immunoassay suitable for a simple, rapid, and reliable screening of CRP in serum.

화원유원지의 개발계획에 관한 연구

김용수(Kim Yong Soo),임원현(Lim Won Hyeon),이제화(Lee Je Wha) 한국전통조경학회 1994 한국전통조경학회지 Vol.12 No.2

N/A N/A

근위부 내경동맥 폐쇄의 뇌졸중과 측부 순환 양상

강동화,조용진,노재규 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

자동화학분석기 Cobas Integra를 이용한 화학검사측정에 대한 평가

이용화,정규영,박은주,김종원 대한임상병리학회 1997 대한임상병리학회지 Vol.17 No.5

페파 크로마토그래피에 의한 各種 果實중의 糖類 分析

李用華 春川敎育大學 1983 論文集 Vol.23 No.-

1. Paper chromatography에 의한 各種 果實들 中에는 살구에는 Fructose가 없고 매실에는 Glucose가 없는 外에 모든 果實에는 Glucose와 Fructose는 모두 含有되어 있다. 2. Sucrose는 산딸기, 참외, 수박, 오디, 도마도, 버치, 앵두, 매실, 포도(赤), 귤등에는 없고 사과, 머루, 배, 포도, 복숭아, 바나나, 메론 등에 나타났다. 卽 작은 열매에는 含有되어 있지 않으나 나무에서 생성되는 果實들은 大體로 Sucrose가 含有되어 있다. 3. Xylose는 新鮮하게 處理된 試料에서만 나타났다. 사과, 복숭아, 머루, 포도, 감등에서 나타났으며 다른 열매 등에서는 나타나지 않았다. Aniline Oxalate매 의하여 Pink color로 發色되었다가 곤 消失되므로 確認하는데 좀 難點이 있다. 4. 모든 果實 中에도 머루와 靑포도에는 Glucose, Fructose, Arabinose 外에도 더욱 몇개의 많은 糖類들이 含有되어 있다.

대전지역 일부 청소년의 식생활습관과 체격과의 관계

변화봉,권윤형,이태용 韓國學校保健學會 2001 韓國學校保健學會誌 Vol.14 No.2

This study investigates the effect of adolescent dining habits on their physical development. We selected 200 students from the three high schools in Taejon, and surveyed with a questionnaire and collected data concerning physique from school health records. The results obtained from this study were as follows: 1. A point of intersection of two development trends of male and female students' heights was between sixth grade of elementary school and first grade of middle school. Two development trends of weights didn't have a point of intersection and male students' development trend of weights was a little higher than that of female students. 2. There were significant differences in the weight and BMI of male students. The 'regular dining' group had heavier weight and higher BMI than the other group, and the 'Eating only favorite food' group had lower weight and BMI than the other group. In female, heights of 'three meals a day' group were higher than that of 'two meals a day' group and the more snacks per day, the higher students' height was. 3. The 'regular exercise' group had significantly heavier weight and higher BMI than the other group amount females. 4. 'Regular dining' and 'Eating habits' had a positive linear relationship with male students' weight and BMI. 'Number of times of snacking' has a positive linear relationships with female students' height and a negative linear relationship with female students' BMI. Since dining habits of adolescents have not only an effect on their development but also later adults' health, to develop good habits of health and dining is a critical issue.

Weimar憲法의 成立과 그 特色

金龍華 호남대학교 1986 호남대학교 학술논문집 Vol.7 No.1

Hier sind insbesondere die folenden als die wichtigsten Probleme untersucht und analysiert worden : 1) revolutionare Vorgange in Jahre 1918 und die Regierungen unter dem Ratsystem, 2) Zusammensetzung der Verfassunggebenden deutschen Nationalversammlung und die Gestaltung der Notverfassung, 3) Verkundigung der neuen Verfassung des deutschen Reiches Vom 11. Ang. 1919, 4) Charakter und Inhalte der Weimarer Verfassung. Zusammenfassend sind die folgenden Grundzuge der Weimarer Verfassung bemerkenswert: 1) unitarische Neigungen und der Zusammenbruch der Partikularismus, 2) die Schwierigkeit des deutschen Parlamentarismus wegen der Zersplitterung der Parteien und des Miβlingens der Unmittelbaren Demokratie, 3) das Versagen des Parlamentarismus und die daraus folgende Prasidialdiktatur auf Grund des Art, 48. 4) machtvakuum und ein starkes Ansteigen des Nationaldozialismus.

전자 상거래의 성공요인에 관한 연구

李庸和 충주대 2001 한국교통대학교 논문집 Vol.36 No.1

With the development of Internet technology, the use of open global communication network used as a Business Purpose.The technology and system development of internet move the traditional offline commerce into online and which create new business area called Electronic Commerce. Electronic Commerce become a new business model that can be overcome the restriction of time and space.Thus many country in the world promote Electronic Commerce as a tool of strengthening national power. At this paper, study the basic understanding and sucessful factor of electronic commerce

현탁용매에 따른 전기영동 초전도 후막의 영향

소대화,박정철,이영매,조용준,Korobova, Natalya 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

For the research of the effects on suspension solution with YBcO and BSCCO for elcectrophoretic deposition to prepare superconducting thick film wire, the preheating technique for the superconducting powders in vaccum system was used with various solvent solutions for electrophoresis such as acetone, toluen and buthanol was investigated. As a result, it was useful to remove the influence of remaining and adsorbed solvent solution which was existed between and on the particle surfaces when the specimens of superconducting wire by electrophoresis were treated in vacuum of 10-3 Torr and temperature around 200℃ in bell-jar system. Of the prepared superconducting wire samples, the critical current density, Jc was measured by 4-point prove method in liquid N₂, and at the value of 10³to 10⁴A/㎠, respectively, for the YBCO and BSCCO superconducting wires.

전략정보시스템에 관한 연구

李庸和 忠州大學校 1996 한국교통대학교 논문집 Vol.31 No.1

Strategic Information System is a joined system of Information technology and Strategic Management theory which was introduced by M. E. Porter. The more the society developed rapidly, the more the management environment become complicated, with the ordinary management system, the company cannot survive anymore. As that reason, lately at the developed country, lots of companys use the strategic information system for achieving the relative advantage in management. In this research, strategic management system is suggested as a strategic management of a company.