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Downregulation of UHRF1 promotes EMT via inducing CXCR4 in human cancer cells.
Jung, Yi-Deun,Shim, Jae-Woong,Park, Seong-Joon,Choi, Si Ho,Yang, Kwangmo,Heo, Kyu,Park, Moon-Taek Lychnia 2015 International journal of oncology Vol.46 No.3
<P>Activation of epithelial-mesenchymal transition (EMT) is important for malignant tumor progression exhibiting migratory and invasive properties. UHRF1 (ubiquitin-like, with PHD and RING finger domains 1), as an epigenetic regulator, plays a crucial role in DNA CpG methylation, chromatin remodeling and gene expression. Many studies demonstrated that UHRF1 is aberrantly expressed in various types of human cancer. However, the precise role of UHRF1 in human cancers remains highly controversial. In the present study, we found that downregulation of UHRF1 enhances the migratory and invasive properties of human cancer cells by inducing EMT, and that the CXCR4 signaling pathway is strictly necessary for UHRF1 deficiency-mediated induction of EMT. Downregulation of UHRF1 induced the expression of the EMT-regulating transcription factors, Zeb1, Slug and Snail and then led to decreased protein level of E-cadherin, and increased protein level of N-cadherin and vimentin, including increased migratory and invasive properties of human cancer cells. In addition, siRNA targeting of Zeb1 or Snail effectively attenuated UHRF1 deficiency-induced EMT, but siRNA targeting of Slug did not, indicating that Zeb1 and Snail play key roles in this event. Moreover, downregulation of UHRF1 induced the expression of CXCR4 in HepG2 cells. siRNA targeting of CXCR4 greatly suppressed the UHRF1 deficiency-induced EMT, as evidenced by a reversal of expression patterns of Snail and Zeb1, and by reduced migratory and invasive properties of HepG2 cells. In conclusion, our results demonstrate that downregulation of UHRF1 contributes to the induction of EMT in human cancer cells via the activation of CXCR4 signaling pathway. Our observation also suggests that UHRF1 may play a pivotal role in suppressing the malignant alteration of cancer cells.</P>
Retroelements: molecular features and implications for disease.
Jung, Yi-Deun,Ahn, Kung,Kim, Yun-Ji,Bae, Jin-Han,Lee, Ja-Rang,Kim, Heui-Soo Genetics Society of Japan 2013 Genes & genetic systems Vol.88 No.1
<P>Eukaryotic genomes comprise numerous retroelements that have a major impact on the structure and regulation of gene function. Retroelements are regulated by epigenetic controls, and they generate multiple miRNAs that are involved in the induction and progression of genomic instability. Elucidation of the biological roles of retroelements deserves continuous investigation to better understand their evolutionary features and implications for disease.</P>
No expression of porcine endogenous retrovirus after pig to monkey xenotransplantation
Seongsoo Hwang,Yi-Deun Jung,Kahee Cho,Sun-A Ock,Keon-Bong Oh,Heui-Soo Kim,Ik-Jin Yun,Curie Ahn,Jin-Ki Park,Seoki Im 한국실험동물학회 2014 Laboratory Animal Research Vol.30 No.2
This study was performed to investigate the expression of two porcine endogenous retrovirus (PERV) elements, PERV gag and full-length conserved PERV, in blood cells collected periodically from organrecipient monkeys that underwent pig to non-human primate xenotransplantation. The heart and kidney—respectively acquired from α-1,3-galactosyltransferase knockout (GT-KO) pigs that survived for24 and 25 days—were xenografted into cynomolgus monkeys. The two PERV elements expressed in the xenografted GT-KO pig organs were not present in the blood cells of the recipient monkeys. In the present study, we deduced that PERVs are not transmitted during GT-KO pig to monkey xenotransplantation.
Genetic structure and variability of the working dog inferred from microsatellite marker analysis
Kwon, Yun-Jeong,Choi, Bong-Hwan,Eo, Jungwoo,Kim, Choongrak,Jung, Yi-Deun,Lee, Ja-Rang,Choi, Yuri,Gim, Jeong-An,Lee, Dong-Hoon,Ha, Ji-Hong,Kim, Dae-Soo,Huh, Jae-Won,Kim, Tae-Hun,Seong, Hwan-Hoo,Kim, He Springer-Verlag 2014 Genes & Genomics Vol.36 No.2
Working dogs serve as military watch dogs, search dogs, rescue dogs, and guide dogs with un-come-at-able character. They are drafted by in-training examination including concentration, capacity for locomotion, boldness and earthly desires. In this study, genetic diversity and relationships among two groups of working dogs (pass and fail group in-training examination) were assessed based on 15 microsatellite markers in 25 individuals of working dogs (military watch dogs and Korean search dogs). For the 15 microsatellite markers, the values of allelic richness (A (R) ) ranged from 2.21 (pass group) to 1.60 (fail group) in military watch dogs, while A (R) ranged from 2.79 (pass group) to 2.72 (fail group) in Korean search dogs. Among 52 different alleles of military watch dogs, 22 alleles were detected in pass group only, while 8 alleles in fail group only. In case of Korean search dogs, 3 alleles were observed in pass group only, while 13 alleles in fail group only. These group-specific unique alleles reflect good biomarker for selecting working dogs (military watch dogs and Korean search dogs), indicating that those group specific microsatellite alleles could separate working dogs to be pass or fail group in out-training dog population. Taken together, this study demonstrates the feasibility of microsatellite analyses for the selection of superior working dogs objectively. Furthermore, this approach could be used for the proper selection of working dogs in combination with in-training examination.