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Kim, Wonduck,Park, Yu Ri,Im, Seonghun,Kim, Dockyu,Kim, Si Wouk Kluwer Academic Publishers 2012 Biotechnology letters Vol.34 No.9
<P>p-Hydroxybenzoate hydroxylase (pobA) and m-hydroxybenzoate hydroxylase (mobA) genes, from the moderate halophile Chromohalobacter sp. HS-2, were expressed and characterized. Solubilities of overexpressed recombinant MobA and PobA were enhanced by the induction of the heat-shock proteins DnaJ and DnaK. Each MobA and PobA maintained stable activity under high NaCl concentrations. V (max) and K (m) values for MobA with m-hydroxybenzoate were 70 μmol min(-1) mg(-1) protein and 81 μM, respectively. Similarly, those of PobA with p-hydroxybenzoate as substrate were 5 μmol min(-1) mg(-1) protein and 129 μM, respectively. The Escherichia coli expression system, including induction of heat shock proteins, was used to convert hydroxybenzoates into protocatechuate (3,4-dihydroxybenzoate) and revealed that resting cells harboring mobA converted 15 mM m-hydroxybenzoate to 15 mM protocatechuate while those harboring pobA converted 50 mM p-hydroxybenzoate to 35 mM protocatechuate at 30 C, respectively.</P>
Azizi, Aqil,Kim, Wonduck,Lee, Jung Hyun Rapid Communications of Oxford Ltd in association 2016 World journal of microbiology biotechnology Vol.32 No.10
<P>Mesophilic and thermophilic anaerobic digesters (MD and TD, respectively) utilizing Gracilaria and marine sediment as the substrate and inoculum, respectively, were compared by analyzing their performances and microbial community changes. During three successive transfers, the average cumulative methane yields in the MD and TD were 222.6 +/- 17.3 mL CH4/g volatile solids (VS) and 246.1 +/- 11 mL CH4/g VS, respectively. The higher hydrolysis rate and acidogenesis in the TD resulted in a several fold greater accumulation of volatile fatty acids (acetate, propionate, and butyrate) followed by a larger pH drop with a prolonged recovery than in the MD. However, the operational stability between both digesters remained comparable. Pyrosequencing analyses revealed that the MD had more complex microbial diversity indices and microbial community changes than the TD. Interestingly, Methanomassiliicoccales, the seventh methanogen order was the predominant archaeal order in the MD along with bacterial orders of Clostridiales, Bacteriodales, and Synergistales. Meanwhile, Coprothermobacter and Methanobacteriales dominated the bacterial and archaeal community in the TD, respectively. Although the methane yield is comparable, both MD and TD show a different profile of pH, VFA and the microbial communities.</P>
한반도 주변 해역으로부터 혐기성 미생물의 분리 및 분리 미생물의 특성 분석
김원덕,이정현,권개경,Kim, Wonduck,Lee, Jung-Hyun,Kwon, Kae Kyoung 한국미생물학회 2016 미생물학회지 Vol.52 No.2
Marine bacteria have represented unique physiologies and products which are not discovered from terrestrial organisms. There has been great interest to utilize and develop marine bacteria in many industrial sectors. Recently, we isolated and characterized anaerobic bacteria from various marine environments in Korea to search organic acids fermenting strains. From our enrichment performed under anaerobic condition, 65 strains were isolated and identified by the 16S rRNA gene sequence analysis. Among them, eleven strains were selected for phylogenetical and biochemical analysis. All tested strains were affiliated with Class Clostridia except one with Class Bacteroidia. Most of strains produce acetate (6 strains) with butyrate (2 strains) and/or formate (4 strains). Strain MCWD5 transformed 40% of glucose to extracellular polymeric substances. These results indicate that many novel anaerobic microorganisms which have great potential in commercial application are distributed in the marine environments of Korean Peninsula. 유기산을 생산하는 발효미생물을 획득하기 위해 갯벌, 심해, 염전 등의 퇴적토와 해초시료 등의 시료에 대해 methanogen 배지, acetogen 배지, Clostridium용 배지 등을 이용하여 농후 배양을 실시하였다. 총 8개 시료로부터 65주의 혐기성 미생물을 분리하였으며 이 중 신규성이 높거나 활용성이 높다고 알려진 11종에 대해 계통분석, 성장 양식(growth pattern), 유기산 생산 평가 등을 시도하였다. 분석이 수행된 균주 중 Bacteroidia 강에 속하는 1주 외에는 모두 Clostridia 강에 속하였으며 성장속도는 $1.2h^{-1}$ 이상이었다. 분석이 수행된 7종 중 6종은 아세트산을 생성하였으며, 부가적으로 2균주는 부틸산을, 4균주는 개미산을 생산하였다. 또한 MCWD5 균주는 제공된 포도당의 약 40%를 세포외 고분자물질로 전환시키는 것으로 나타났다. 본 연구를 통하여 국내 연안해역에서 분리된 신규 혐기성 미생물들은 유기산, 고분자 다당류를 생산하는 등 높은 응용성을 지님을 확인할 수 있었다.
박선준(Sunjoon Park),이상직(Sangjik Lee),정원덕(Wonduck Jung),정해도(Heado Jeong) 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.6
Recently, according to the development of semiconductor industry, needed to high-integration and highfunctionality. These changes are required for silicon wafer of large scale diameter and precision of TTV (Total Thickness variation). So, in this research, suggest that the method of monitoring system is using the motor current and AE sensor. This method is needed for observation of silicon wafer grinding process. This monitoring system is consisted of two parts. One is sensing part, the other is analysis part. Motor current sensor is used for detection of low frequency. AE sensor is used for detection of high frequency. Received original signal is converted to the digital, then, it is calculated RMS values, and then, it is analyzed in the computer. In this research, we tried to monitoring of motor current change and AE change. And then, it will be applied to analysis for silicon wafer grinding process.