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( Agata Malinowska ),( Sylwia Kowalczyk1 ),( Zdzis Aw Targo Ski ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.3
The identification of bacteriophage proteins on the surface of Lactobacillus rhamnosus Pen was performed by LC-MS/MS analysis. Among the identified proteins, we found a phage-derived major tail protein, two major head proteins, a portal protein, and a host specificity protein. Electron microscopy of a cell surface extract revealed the presence of phage particles in the analyzed samples. The partial sequence of genes encoding the major tail protein for all tested L. rhamnosus strains was determined with specific primers designed in this study. Next, RT-PCR analysis allowed detection of the expression of the major tail protein gene in L. rhamnosus strain Pen at all stages of bacterial growth. The transcription of genes encoding the major tail protein was also proved for other L. rhamnosus strains used in this study. The present work demonstrates the spontanous release of prophage-encoded particles by a commercial probiotic L. rhamnosus strain, which did not significantly affect the bacterial growth of the analyzed strain.
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Photochemistry of Some 1,1'-Dicycloalkenyls. The Mechanism of Sensitized Photocyclization
심상철,Sang Chul Shim,Frederick P. Targos Korean Chemical Society 1976 대한화학회지 Vol.20 No.3
1,1'-디시클로알케닐의 광고리화 반응을 형광 퀘ㄴ칭, 증감제등을 써서 연구하였다. 증감제를 쓰는 경우 이 디엔의 광고리화 반응은 비틀린 삼중상태나 평면구조의 트란스 삼중상태보다 높은 에너지 준위에 있는 평면구조의 s-시스 삼중상태에서 일어남을 알았다. The photocyclization of 1,1'-dicycloalkenyls (1,1'-dicyclohexenyl, 1,1'-dicyclopentenyl, 1,1'-dicycloheptenyl and 1,1'-dicyclooctenyl) is studied. Irradiation at liquid nitrogen temperature does not show trans double bond band in IR spectra. Even though naphthalene and pyrene fluorescence are quenched very efficiently by 1, 1'-dicycloalkenyls, no or trace amount of cyclobutenes are accompanied. When acetophenone or benzophenone is used, cyclobutene is obtained only from 1,1'-dicyclohexenyls, 1,1'-dicycloheptenyl giving some adducts. Naphthalene and pyrene sensitizes adduct formation but not cyclization. From above observations, it is concluded that photocyclization occurs from the planar s-cis triplet state rather than twisted triplet, singlet excited state or vibrationally excited ground state in sensitized photocyclization of 1,1'-dicycloalkenyls.
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