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Tamires Carvalho dos Santos,Nadabe dos Santos Reis,Tatielle Pereira Silva,Fabiano de Paula Pereira Machado,Renata Cristina Ferereira Bonomo,Marcelo Franco 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.1
Prickly palm cactus husk was used as a solid-state fermentation support-substrate for production of the ligninolytic enzymes laccase, peroxide manganese, and lignin peroxidase by Aspergillus niger. Effects of water activity, temperature, and fermentation time on enzymatic production were evaluated using a central composite rotatable design. Response surface methodology revealed that maximum enzyme production was achieved at 73.38 h of fermentation, a water activity of 0.87 Aw, at 28.74oC for laccase, at 65.33 h, 0.89 Aw, and 28.96oC for lignin peroxidase, and at 70.44 h, 0.91 Aw, and 28.84oC for manganese peroxidase. Optimized enzyme production was 9,023.67 UI/L for laccase, 2,234.75 UI/L for lignin peroxidase, and 8,534.81 UI/L for manganese peroxidase. Thermostability and pH stability were observed for all enzymes. Enzymatic deactivation kinetic experiments indicated that enzymes remained active after freezing of crude extracts.
Tamires Carvalho dos Santos,George Abreu Filho,Ana Carolina Oliveira,Thiago José Onório Rocha,Fabiano de Paula Pereira Machado,Renata Cristina Ferreira Bonomo,Katia Iro Altides Mota,Marcelo Franco 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.1
This study aimed at demonstrating the effects of fermentation time (24, 48, 72, 96, and 120 h) and water activity (0.943, 0.970, and 0.985) on the production of cellulolytic enzymes by solid-state fermentation of purple mombin (Spondias purpurea L.) residue using Aspergillus niger. The fermentation was carried out at 35oC and the enzyme production was measured as endoglucanase and total cellulose activities. The optimum condition for endoglucanase was water activity 0.974 and 93.8 h of fermentation, reaching a production of 3.21 U/g of residue;whereas for total cellulase it was 0.958 and 79.4 h achieving 12.1 U/g of residue. Fermentation time had a greater effect on the endoglucanase activity, while water activity had a more significant influence on the total cellulase activity. Endoglucanase had optimum activity at temperature of 50oC and pH 5.0. Although cellulase total optimum activity was also at pH 5.0, the maximum activity was at 60oC.
Patterns in solute chemistry of six inlet streams to Lake Hövsgöl, Mongolia
Tamir Puntsag,Jeffrey S. Owen,Myron J. Mitchell,Clyde E. Goulden,Patrick J. McHale 한국생태학회 2010 Journal of Ecology and Environment Vol.33 No.4
A number of characteristics of the Lake Hövsgöl watershed, such as the lake’s location at the edge of the Central Asian continuous permafrost zone, provide a unique opportunity to evaluate possible anthropogenic impacts in this remote area in northern Mongolia. In this study, we compared stream solute concentrations in six sub-watersheds in the Lake Hövsgöl watershed. Water samples were collected during the summer months between 2003 and 2005. Concentrations of Cl⁻ ranged from 9.8 to 51.3 μmol/L; average nitrate concentrations were very low and ranged from undetectable to 1.1 μmol/L and average SO₄²⁻ concentration at sampling stations with minimal animal grazing ranged from 66 to 294μmol/L. Average dissolved organic carbon (DOC) concentrations ranged from 642 to 1,180 μmol C/L. We did not find statistically significant differences in DOC concentrations among the six streams, although DOC concentrations tended to be higher in the two northernmost streams, possibly related to differences in the active layer above the permafrost. Dissolved organic nitrogen (DON) concentrations were correlated with DOC concentration, and followed the same spatial pattern as those for DOC. In streams in this remote watershed, total dissolved nitrogen was made up of mostly organic N, as has been found for other regions distant from anthropogenic N sources. Overall, these results suggest that future research on the dynamics of DOC and DON in this watershed will be especially insightful in helping to understand how changes in climate and land use patterns will affect transformations, retention, and export of dissolved organic matter within these sub-watersheds in the Lake Hövsgöl region.
A STUDY ON THE UNDERLYING FACTORS AFFECTING HOUSEHOLDS’ WASTE SORTING BEHAVIOR AT SOURCE IN MONGOLIA
Tamir Oyunbileg,Dolgormaa Enkhbaatar,Nyamkhuu Byambadolgor 글로벌지식마케팅경영학회 2023 Global Marketing Conference Vol.2023 No.07
The purpose of this research is to better understand what factors influence on households’ waste sorting behavior at source in Mongolia. To accomplish the goal, this study conducted online survey with self-report questionnaire method and the 353 participants’ data were collected through Facebook groups and pages related to house owners’ associations and waste recycling related non-profit organizations. The results show that the underlying factors determining the intention of waste sorting at source are (1) motivational bases such as prior beliefs and social norms; (2) and an ability/knowledge about the waste sorting at source. Furthermore, the intention is crucial predictor of the real behavior of waste sorting at source, but the most important one is an opportunity to do so. The conclusions and the recommendations are provided.
Patterns in solute chemistry of six inlet streams to Lake Hövsgöl, Mongolia
Puntsag, Tamir,Owen, Jeffrey S.,Mitchell, Myron J.,Goulden, Clyde E.,McHale, Patrick J. The Ecological Society of Korea 2010 Journal of Ecology and Environment Vol.33 No.4
A number of characteristics of the Lake H$\ddot{o}$vsg$\ddot{o}$l watershed, such as the lake's location at the edge of the Central Asian continuous permafrost zone, provide a unique opportunity to evaluate possible anthropogenic impacts in this remote area in northern Mongolia. In this study, we compared stream solute concentrations in six sub-watersheds in the Lake H$\ddot{o}$vsg$\ddot{o}$l watershed. Water samples were collected during the summer months between 2003 and 2005. Concentrations of $Cl^-$ ranged from 9.8 to $51.3\;{\mu}mol/L$; average nitrate concentrations were very low and ranged from undetectable to $1.1\;{\mu}mol/L$ and average ${SO_4}^{2-}$ concentration at sampling stations with minimal animal grazing ranged from 66 to $294\;{\mu}mol/L$. Average dissolved organic carbon (DOC) concentrations ranged from 642 to $1,180\;{\mu}mol$ C/L. We did not find statistically significant differences in DOC concentrations among the six streams, although DOC concentrations tended to be higher in the two northernmost streams, possibly related to differences in the active layer above the permafrost. Dissolved organic nitrogen (DON) concentrations were correlated with DOC concentration, and followed the same spatial pattern as those for DOC. In streams in this remote watershed, total dissolved nitrogen was made up of mostly organic N, as has been found for other regions distant from anthropogenic N sources. Overall, these results suggest that future research on the dynamics of DOC and DON in this watershed will be especially insightful in helping to understand how changes in climate and land use patterns will affect transformations, retention, and export of dissolved organic matter within these sub-watersheds in the Lake H$\ddot{o}$vsg$\ddot{o}$l region.
Kh. Tamir,O. Altangoo,M. Munkhbaatar 한국환경생태학회 2015 한국환경생태학회 학술대회지 Vol.2015 No.2
Our professors and teachers have been cooperated with professors and researchers of National Sciences Museum of Korea for the project of “Biodiversity of Mongolian” since 2012. This project is to study for Mongolian biodiversity by Mongolian and Korean scientists and to support advanced training for our professionals, to increase capabilities and training new methodologies for students and to publish articles from the conducted research. Our researches conducted following areas such as in Ikh Nart Nature reserve area in 2013, Khan Khentii strictly protected area in 2014, our university’s Shatan research camp in 2014, and Kherlen Toonot Nature reserve area in 2015. From these studies, we published articles of “Biodiversity of Ikh Nart Nature reserve” and “Biodiversity of Shatan area” in Korean journals. Our teachers anticipated for publishing guidebook “Methods to preparing natural taxidermy and herbarium” in Mongolian prepared by Korean scientists to useful for students in laboratory and field. Teachers are visited in National science museum of Korea, such as Mr. J.Ariunbold studied in 2013 and Dr. M.Munkhbaatar visited for 14-30 days to develop samples collected from field research. Our teaching assistant G.Onolragchaa is enrolled as doctoral student for Chunnam University in 2015 according to this project grant. Also Mr. P.Erdenetushig and one student are planning to visit in October for developing collected samples. In 2015, School of Mathematics and Natural Sciences is willing to establish “Mongolian and Korean monitoring center”, it’s in compromise of both side, and Mongolian National University of education is prepared a room for this center. Коrean colleague provided USD 10.000 worth of new equipment such as electric motor, boryoscope, tent trap, Sherman trap, aquatic net, sweep net, glass vials and bottles for research and teaching of MNUE. Framework of Mongolian-Korean cooperative research project “Biodiversity of Mongolia” was held a conference topic on “Biological resource management and remote sensing survey” in Ulaanbaatar 2014 and 2015. Our scientists are planning future collaboration with scientists of Korean ocean research institute, Ornithological Society of Korea, Korea Institute of Environmental Ecology. Official cooperative agreement with MNUE To cooperate with teachers of MNUE for maintaining Mongolian Natural History Museum and public education To initiate Korean- Mongolian cooperative non-governmental organization To implement a project for public outreach (forest and animal breeding) To establish long term monitoring site in special protected area Genetic research on problems of plant and animal classification; To train graduate students for modern research methods Genetic study on less studied or rare species of museum specimen Genetic and morphological study on vertebrates Scale up biodiversity study and do monitoring (already done Ikh Nart and Shatan) To extend research projects in Mongolia and do long term monitoring study