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Yoon, Jeong,Juhn, Kyoung-Mi,Yoon, San-Hyun,Ko, Yong,Lim, Jin-Ho The Korean Society for Reproductive Medicine 2017 Clinical and Experimental Reproductive Medicine Vol.44 No.1
Objective: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of $Ca^{2+}$ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). Methods: MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a $Ca^{2+}$ chelator to investigate the effect of $Ca^{2+}$ oscillations on their maturation. Results: As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the $Ca^{2+}$ chelator-treated group. Conclusion: The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by $Ca^{2+}$ chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular $Ca^{2+}$ oscillations driven by fertilization.
만성 요통환자의 복부 심부근과 표재근을 중심으로 한 운동 효과 비교
김진산,이창현,조미주,임연욱,정취산,김선엽 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.1
Objectives: To compare the efficacy of two types specific exercise intervention (deep and superficial abdominal muscle exercises) in the treatment of patients with chronic low back pain (CLBP). Design: A randomized, comparative, repeated-measures. Subjects: Twenty-four patients with CLBP (at least for 3 months), mean age±SD=51.58±16.21 (yrs). Methods: Twenty-four patients with this condition were assigned randomly to two treatment groups. Both first and second group underwent 3 weeks specific exercise treatment program. The first group specific trained for the deep abdominal muscles. The second group specific trained for the superficial abdominal muscles. Results: After intervention, the first group showed a statistically significant reduction in pain intensity and functional disability levels (p<.05). The second group showed no significant change in these parameters after intervention (p>.05). Conclusions: According to results, a "deep abdominal muscle exercises" treatment approach appears more effective than a "superficial abdominal muscle exercises" treatment in patients with CLBP.
Paraquat의 殘留毒性에 미치는 완충액의 種類 및 光照射의 影響
金英美,申應男,李錦美,全相愛 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-
완충액의 種類 및 光照射가 paraquat의 毒性低下에 미치는 影響을 검토한 結果 使用한 3種(sodium citrate, sodium phosphate, tris-HCl)의 완충액 中 sodium phosphate buffer에 paraquat를 용해하였을 때가 가장 큰 幅으로 毒性이 低下되었다. 經時的으로는 1日만에 約 60%의 細菌이 生育하였고 4日째 내는 95% 以上의 細菌이 生育함으로써 sodium phosphate buffer 中에서는 約 4日만에 paraquate의 毒性이 거의 消滅되었다. 한편, 暗所에 保有한 paraquate에 比하여 自然光구에서 1.3倍 紫外線 照射구에서 約 2倍의 生有율이 檢出되므로 光照射에 의하여 paraquat의 毒性이 보다 빨리 消滅되는 것으로 判斷되었다. Experiment was carried out to understand the effect or buffer solution and light irradiation on toxicity of paraquat against microorgnism. Paraquat most rapidly lost its toxicity in the sodium buffer solution among three kinds of tested solutions. (sodium citrate, sodium phosphate and tris-HCI) By previous incubation of paraquat in the solution for 24 hours, the organism showed about 60 percent of survival rate. The paraquat lost almost of its toxicity as showing at least 95 per cent of survival by continuous incubation for 4 days. The organism showed a survival rate of 1.3 times higher by natural light irradiation and 2.0 times higher by ultraviolet ray irradiation than control.
트레드밀 걷기 운동 시 무게부하의 차이가 호흡순환기능 및 대사기능에 미치는 영향
김용규,이채산,김자영,박미숙,김광중,이광호 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1
The purpose of this study was to compare on cadiopulmonary and metabolism function of 10㎏ weight loading group(10 females) and W university students with non weight loading group(10 females. Each group did treadmill walking exercise for twenty minutes by the modified balke protocol. Heart rate(HR), systolic(SBP), MVO₂, and VO₂max were analyzed using 2×2(groups×during) repeated measures analysis of variance (MANOVA). And peak blood velocity(PBV) and MBV were analyzed by using 2×2(groups×during) repeated measures analysis of variance (MANOVA). Lactate and glucose were amalyed by separate 2×2 (groupxduring) analysis of variance(ANOVA). Results of this study suggest that: The change of HR, SBP and MVO₂ in experience and control group showed the significant(p<.05) increase during walking exercise. Variables between experience and control group showed not significant level. But VO₂ in each group showed the significant(p<.001) increase during walking exercise. Differenc among each group showed significant(p<.001) level. Lactate and glucose level in each group showed significant(p<.001, p<.05) increase during walking exercise. Differences among each group showed not significant level. PBV and MBV in each group significant(p<.001) increase during walking exercise. Difference among each group showed significant(p<.05) level. According to these results, weight loading walking exercise considering safety promotes to cardiovascular function and it is expected to be an effective exercise methods able to improve the physical inefficiency of long time walking type.
Sodium Trans-Bis(methyliminodiacetato)Chromate(Ⅲ)의 두가지 다른 空間群 P1 ̄와 A2/n間의 相互 變換
李珍昊,宋柱昊,崔成山,郭相姬,吳美蘭,박성진,李揆旺,徐廷善,徐日煥 충남대학교 1996 忠南科學硏究誌 Vol.23 No.1
The structure of sodium trans-bis(methyliminodiacetato) chromate(Ⅲ), C_10H_14O_8N_2CrNa, has been elucidated with two space groups P 1-; a=8.9856(6), b=13.308(1), c=6.261(1)Å, α=76.38(1), β=110.361(9), r=107.394(6)˚, V=662(2)Å^3, Z=2, μ=0.47mn^(-1), d=1.831Mg/㎥, R=0.0295 for 1901 independent reflections with F^2>2σ(F^2), and A2/n; a=13.65(5), b=6.261(1), c=16.85(6)Å, β=113.05(1)˚, V=1324(7)Å^3, Z=4, R=0.0287 for 1013 independent reflections with F^2>2σ(F^2). There are two different asymmetric half molecules in triclinic system and one asymmetric half molecule in monoclinic system. The structures of three different molecules are very similar in conformations. The four carboxyl O atoms and the two imino N atoms in each molecule produce a distorted octahedral coordination geometry around the Cr atom. The average distances of Cr-O(carboxyl) and Cr-N(imino) are 1.956 and 2.061Å respectively. Molecules are held together by ionic forces. In this paper, it has been also shown that the cell constants, Miller indices, atomic coordinates, lattice directions and reciprocal lattices of the two space groups can be mutually transformed.
한국산 Gelechiinae아과(나비목, 뿔나방과)의 3 미기록종 보고
San-Mi LEE 한국응용곤충학회 2000 INSECTA KOREANA Vol.17 No.1
한국산 Gelechiinae아과의 3종, Angustialata gemmellaformis Omelko(가는날개뿔나방), Chorivalva grandialata Omelko(얼룩무늬뿔나방), 그리고 Eulamprotes wikella(Linnaeus)(별꽃뿔나방)을 미기록종으로 보고하며, 이들에 대한 간략한 재기재를 포함하여 성충과 암수 생식기를 도해하였다. Three species of Gelechiinae, Angustialata gemmellaformis Omelko, Chorivalva grandialata Omelko, and Eulamprotes wilkella (Linnaeus), are reported for the first time from Korea. Photos of imagos, illustrations of the male and female genitalia, and wing venations are provided.
한국산 Gelechiinae아과(나비목, 뿔나방과)의 3 미기록종 보고
San-Mi LEE 한국응용곤충학회 2000 Insecta Koreana Vol.17 No.1
한국산 Gelechiinae아과의 3종, Angustialata gemmellaformis Omelko(가는날개뿔나방), Chorivalva grandialata Omelko(얼룩무늬뿔나방), 그리고 Eulamprotes wikella(Linnaeus)(별꽃뿔나방)을 미기록종으로 보고하며, 이들에 대한 간략한 재기재를 포함하여 성충과 암수 생식기를 도해하였다. Three species of Gelechiinae, Angustialata gemmellaformis Omelko, Chorivalva grandialata Omelko, and Eulamprotes wilkella (Linnaeus), are reported for the first time from Korea. Photos of imagos, illustrations of the male and female genitalia, and wing venations are provided.
Peucedanum japonicum Thunb. ethanol extract suppresses RANKL-mediated osteoclastogenesis
Kim, Jeong-Mi,Erkhembaatar, Munkhsoyol,Lee, Guem-San,Lee, Jin-Hyun,Noh, Eun-Mi,Lee, Minok,Song, Hyun-Kyung,Lee, Choong Hun,Kwon, Kang-Beom,Kim, Min Seuk,Lee, Young-Rae Spandidos Publications 2017 Experimental and therapeutic medicine Vol.14 No.1
Antimicrobial resistance of streptococci isolated from mastitic bovine milk samples in Korea.
Nam, Hyang-Mi,Lim, Suk-Kyung,Kang, Hyun-Mi,Kim, Jae-Myoung,Moon, Jin-San,Jang, Keum-Chan,Joo, Yi-Seok,Kang, Mun-Il,Jung, Suk-Chan AAVLD 2009 Journal of veterinary diagnostic investigation Vol.21 No.5
<P>The aim of this study was to determine the antimicrobial resistance of streptococci isolated from mastitic bovine milk samples. A total of 178 isolates belonging to 6 different Streptococcus species were examined: S. uberis (n = 99), S. bovis (n = 30), S. oralis (n = 24), S. salivarius (n = 13), S. intermedius (n = 7), and S. agalactiae (n = 5). Only 8.9% (16/178) of the isolates were susceptible to all antimicrobial agents tested in this study, and S. agalactiae and S. intermedius isolates were all resistant to at least 1 antimicrobial agent tested. Overall, the most frequently observed resistance was to tetracycline (61.2%), followed by lincomycin (43.2%), gentamycin (35.3%), oxacillin (34.3%), and erythromycin (28.6%). Cephalothin and penicillin were the only antimicrobial agents to which most of the streptococci (>or=92%) were susceptible. Wide differences in the prevalence of resistance are apparent among the individual species: S. salivarius displayed exceptionally high resistance to cephalothin (23.0%) and oxacillin (76.9%) and S. agalactiae (20%) and S. intermedius (14.2%) to penicillin. Streptococcus salivarius and S. agalactiae were all susceptible to erythromycin, but others showed various rates of resistance ranging from 12.5% to 42.8%. Resistance to 3 or more of 7 antimicrobial agents was observed in all species (37.6%, 67/178).</P>