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Development of a SCAR Marker Linked to Ph-3 in Solanum ssp
Pue Hee Park,Young Chae,Hyun Ran Kim,Kyeong Ho Chung,Dae Geun Oh,Ki Taek Kim 한국육종학회 2010 한국육종학회지 Vol.42 No.2
Late blight caused by Phytophthora infestans is historically a serious epidemic disease in potato and tomato cultivations. Accession L3708 (Solanum pimpinellifolium), a new source for late blight resistance was identified in AVRDC, and carries the resistance gene, Ph-3, incompatible to P. infestans race 3. The AFLP markers linked to Ph-3 were previously developed from the L3708 accession (Chunwongse et al. 2002). To facilitate tomato breeding with the Ph-3 gene, an attempt was made to convert AFLP markers to sequence-characterized amplified region (SCAR) markers. Among 6 AFLP markers, only one AFLP marker, L87, was successfully converted to SCAR marker. The resistance-specific 230 bp AFLP fragment was cloned and sequenced, and the PCR primer amplifying a 123 bp fragment was designed. This SCAR marker could discriminate resistant and susceptible individuals with high stringency. The developed SCAR marker could be used for the marker assisted-selection in tomato breeding programs.
Analysis of Genetic Diversity and Discrimination in Breeding Phalaenopsis Varieties using SSR
Pue Hee Park,Mi-Seon Kim,Byeong Woo Yae,Young-Ran Lee,Pil Man Park 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Korea signed to the international union for the protection of new varieties of plants (UPOV) 2002, it has been increased that the importance of the rights protection for breeder. National institute of horticultural & herbal science (NIHHS) has been bred using crossing and tissue culture since 1992 and released nineteen new Phalaenopsis varieties from 2002 to 2011. This study was conducted to develop DNA markers for discrimination of the Phalaenopsis varieties bred in NIHHS, Korea. Also the genetic relationships among 14 Phalaenopsis varieties were analyzed using simple sequence repeat (SSR) markers. Fifty five polymorphic bands (4.6 per primer) were generated by polymerase chain reaction with selected 12 primers among 111 primers. The dendrogram was constructed by using the UPGMA clustering algorithm based on genetic similarity. The Similarity values among the breeding Phalaenopsis cultivars ranged from 0.593 to 0.945. Fourteen Phalaenopsis cultivars were classified into three major groups at similarity coefficient value of 0.66. Understanding of the genetic diversity could be useful in Phalaenopsis breeding program. We could discriminate these breeding varieties using SSR 20 and SSR 21. These molecular markers could be utilized as a reliable tool for variety discrimination with morphological characterization in breeding Phalaenopsis.