Molecular characterization and expression pattern of a novel Keratin-associated protein 11.1 gene in the Liaoning cashmere goat (Capra hircus)

Mei Jin,Qian Cao,Ruilong Wang,Jun Piao,Fengqin Zhao,Jingai Piao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.3

Objective: An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness. Methods: In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed KAP11.1. On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed using in situ hybridization. Results: Bioinformatics analysis showed that KAP11.1 gene encodes a putative 158 amino acid protein that exhibited a high content of cysteine, serine, threonine, and valine and has a pubertal mammary gland) structural domain. Secondary structure prediction revealed a high proportion of random coils (76.73%). Semi-quantitative RT-PCR showed that KAP11.1 gene was expressed in heart, skin, and liver, but not expressed in spleen, lung and kidney. Real time PCR results showed that the expression of KAP11.1 has a higher expression in catagen than in anagen in the primary hair follicles. However, in the secondary hair follicles, KAP11.1 has a significantly higher expression in anagen than in catagen. Moreover, KAP11.1 gene has a strong expression in inner root sheath, hair matrix, and a lower expression in hair bulb. Conclusion: We conclude that KAP11.1 gene may play an important role in regulating the fiber diameter.

Mitochondrial-Targeted Polyethylenimine Functionalized Graphene Oxide Nanocarrier and Its Anti-Tumor Effect on Human Lung Carcinoma Cells

Mei Jin,Zhiming Liu,Wen Zhang,Haixin Dong,Fang Zhou,Jianfeng Yu,Xinpeng Wang,Zhouyi Guo 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.8

Graphene oxide nanosheet (NGO) was covalently functionalized with positively charged, branched polyethylenimine (bPEI) via an amide bond, coated with serum proteins by electrostatic interaction. Operating as a newly fashioned, multifunctional nanocarrier, the processed NGO showed promise for use in combined gene therapy, chemotherapy, photothermal therapy, bioimaging and as a biosensor of cancer cells. Our current research is focused on the systematic studies of mechanisms of cancer cellular uptake, subcellular location, cytotoxicity and the cellular exclusion of the NGO–bPEI nanocarriers. It was observed that NGO–bPEI accumulated in the mitochondria and that long-term retention of NGO–bPEI led to a decrease in mitochondrial membrane potential while levels of reactive oxygen species increased. The mitochondrial effects associated with long-term retention of NGO–bPEI have potential as a synergistic enhancer of the cytotoxic effects of anti-cancer drugs or genes in human lung carcinoma (A549) cells. This work demonstrated the utility of NGO–bPEI-based multifunctional nanocarriers while detailing the mechanism at the cellular level and providing guidance for further research in cancer therapy.

Two new quinones from the roots of Juglans mandshurica

Mei Jin,Jinfeng Sun,Ren Li,Shengbao Diao,Changhao Zhang,Jiong-Mo Cui,손종근,Wei Zhou,Gao Li 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.9

Two new quinones, 1-hydroxy-5-pentyl-anthraquinone (1) and 4-(5-hydroxy-1,4-dioxo-1,4-dihydronaphthalen- 2-ylamino)-butyric acid methyl ester (2), together with two known quinones, 5-hydroxy-2-(2-hydroxy- ethylamino)-(1,4) naphthoquinone (3) and juglone (4) were isolated from the roots of Juglans mandshurica (Juglandaceae). Their structures were elucidated on the basis of spectral data. Compound 3 was isolated from the Juglans genus for the first time. Compounds 1–4 exhibited significant cytotoxicity towards cultured MDA-MB231, HepG2 and SNU638 cells with IC50 values ranging from 4.46 to 88.47 μM.

Fractional Order Sliding Mode Control for Permanent Magnet Synchronous Motor Speed Servo System via an Improved Disturbance Observer

Weijia Zheng,Runquan Huang,Meijin Lin,Fang Guo,YangQuan Chen,Xiaohong Wang 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.4

A fractional order sliding mode control (FOSMC) method is developed in this paper to deal with the control problem of permanent magnet synchronous motor (PMSM) speed servo system subject to multiple disturbances including model uncertainties, unknown constant disturbances and harmonic disturbances. The lumped exogenous disturbances and uncertainties of the PMSM speed servo are estimated by an improved disturbance observer (DO) and an extended state observer (ESO), respectively. Then, a novel FOSMC law is developed by incorporating the feedforward compensation and a fractional order switching law. The stability of the closed-loop system is established based on Lyapunov stability approach. Under the FOSMC scheme, the tracking performance and robustness of the PMSM servo system are improved simultaneously in the presence of mismatched disturbance torques and measurement noise. The effectiveness and advantages of the proposed method are demonstrated by the PMSM speed regulation experiments and the comparisons with some existing methods.

Fault detection in nonlinear chemical processes based on kernel entropy component analysis and angular structure

Xuefeng Yan,Qingchao Jiang,Zhaomin Lv,Meijin Guo 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.6

Considering that kernel entropy component analysis (KECA) is a promising new method of nonlinear data transformation and dimensionality reduction, a KECA based method is proposed for nonlinear chemical process monitoring. In this method, an angle-based statistic is designed because KECA reveals structure related to the Renyi entropy of input space data set, and the transformed data sets are produced with a distinct angle-based structure. Based on the angle difference between normal status and current sample data, the current status can be monitored effectively. And,the confidence limit of the angle-based statistics is determined by kernel density estimation based on sample data of the normal status. The effectiveness of the proposed method is demonstrated by case studies on both a numerical process and a simulated continuous stirred tank reactor (CSTR) process. The KECA based method can be an effective method for nonlinear chemical process monitoring.

Oxygen Uptake Rate Controlling Strategy Balanced with Oxygen Supply for Improving Coenzyme Q10 Production by Rhodobacter sphaeroides

Ze-Jian Wang,Xingzi Zhang,Ping Wang,Zhiwei Sui,Meijin Guo,Siliang Zhang,Yingping Zhuang 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3

The effects of different oxygen uptake rates (OUR) on the physiological metabolism of Rhodobacter sphaeroides were investigated systematically in 50 L fermenters due to the significant influence on industrial coenzyme Q10 production under oxygen supply limitation. Meanwhile, the seriously decreased oxygen transfer rate caused by the increased broth viscosity was successfully prevented with OUR-directed continuous ammonium sulfate feeding in the late fermentation phase. The statistical analysis results showed that controlling OUR constantly at 45 ± 2.2 mmol/L/h by the oxygen supply level adjustment and the continuous ammonium sulfate feeding could greatly enhance Q10 production. This OUR-Stat controlling strategy successfully achieved the maximal coenzyme Q10 production (2584 ± 82 mg/L), which was 15.4% higher than that of the control. The highest specific CoQ10 content was 25.9 mg/(g DCW)), and the yield of CoQ10 to glucose consumption was up to 19.37 mg/g. These results demonstrated that the optimal OUR-Stat controlling strategy would be effective and economical for improving the industrial CoQ10 production.

Computational Fluid Dynamics Modeling of an Inverted Frustoconical Shaking Bioreactor for Mammalian Cell Suspension Culture

Haifeng Hang,Yuanxin Guo,Jian Liu,Li Bai,Jianye Xia,Meijin Guo,Matthew Hui 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.3

We previously developed an inverted frustoconical shaking bioreactor (IFSB) which had high mammalian cell culture performance when compared with a mechanically stirred tank reactor (STR) or a flat-bottom shaking bioreactor (FBSB). Here, we determined the mixing time (t) and volumetric oxygen transfer coefficient (kLa) of this IFSB at various speeds, and simulated the fluid hydrodynamics, including the shear stress and specific surface area, by computational fluid dynamics. The shortest mixing time was observed in a STR. The maximum kLa value of 12/h was achieved in the IFSB at an aeration rate of 4 L/h, demonstrating that our IFSB has enhanced oxygen transfer capabilities needed to meet the demands of mammalian cells. Simulation studies revealed a 3% greater specific surface area and a 21% lower shear strain in the IFSB compared to an FBSB under the same conditions. Additionally, the conical angle of the vessel, which significantly affected cell growth and recombinant protein production,was tested here. We conclude that, compared to the STR and FBSB, the IFSB has an increased liquid surface area for oxygen uptake and exhaust CO_2 stripping,an enhanced k_La for cell robust growth to a high cell density, and a lower shear stress to alleviate cell damage.