http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Jun Tao ),( Fa-liang An ),( Zheng-hua Pan ),( Yan-hua Lu ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.5
Biofilms are of vital significance in bioconversion and biotechnological processes. In this work, sugarcane molasses was used to enhance biofilms for the improvement of the production of fumigaclavine C (FC), a conidiation-associated ergot alkaloid with strong anti-inflammatory activities. Biofilm formation was more greatly induced by the addition of molasses than the addition of other reported biofilm inducers. With the optimal molasses concentration (400 g/l), the biofilm biomass was 6-fold higher than that with sucrose, and FC and conidia production was increased by 5.8- and 3.1-fold, respectively. Moreover, the global secondary metabolism regulatory gene laeA, FC biosynthetic gene fgaOx3, and asexual central regulatory genes brlA and wetA were upregulated in molasses-based biofilms, suggesting the upregulation of both asexual development and FC biosynthesis. This study provides novel insight into the stimulatory effects of molasses on biofilm formation and supports the widespread application of molasses as an inexpensive raw material and effective inducer for biofilm production.
Fa-yong Liang,Jin Huang,Jun-yao He,Pu Wang 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.5
S-(+)-2,2-dimethylcyclopropanecarboxylic acid (S-(+)-DMCPA) is a key chiral intermediate for the synthesis of Cilastatin. The enzymatic preparation of S-(+)-DMCPA has attracted much attention. In order to improve the activity and stability of Novozyme 435 for enzymatic preparation of S-(+)-DMCPA from 2,2-dimethylcyclopropane carboxylate (DMCPE), the glutaraldehyde modification for Novozyme 435 was investigated and the glutaraldehydemodified Novozyme 435 was used as biocatalyst for the synthesis of S-(+)-DMCPA. The results showed that the modified Novozyme 435 had a better reusing merit than unmodified enzyme. The maximum specific activity was obtained by modification Novozyme 435 with 1.5%glutaraldehyde solution under the conditions of shaking at 200 rpm and 30oC for 45 min. The optimal enzymatic hydrolysis conditions for glutaraldehyde-modified Novozyme 435 were also confirmed. The optimized hydrolytic reaction mixture contained 10 mL potassium phosphate buffer (1.0 mol/L, pH 7.6), 90 mg of DMCPE and 160 mg of glutaraldehyde-modified enzyme, and the reaction was performed at 30oC and 200 rpm for 52 h. The reusing efficiency of modified Novozyme 435 was further evaluated. Under the optimal conditions, the modified enzyme remained 76.0% of its original yield after 10 times reuse,but the optical purity of the product kept intact; whereas the yield of unmodified enzyme reduced to 20.8% of its initial value and the ee value of product decreased a lot to 90.7% after 7 times recycle. These results showed that the modified Novozyme 435 was more cost-effective for the preparation of S-(+)-DMCPA in industrial application.
Li-Jun Jia,Shou-Fa Zhang,Nian-Chao Qian,Xue-Nan Xuan,Long-Zheng Yu,Xue-Mei Zhang,Ming-Ming Liu 대한기생충학열대의학회 2013 The Korean Journal of Parasitology Vol.51 No.2
Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. In the current study, NcSRS2 and NcGRA7 genes were spliced by overlap-extension PCR in a recombinant adenovirus termed Ad5-NcSRS2-NcGRA 7, expressing the NcSRS2-NcGRA7 gene, and the efficacy was evaluated in mice. The results showed that the titer of the recombinant adenovirus was 109TCID50/ml. Three weeks post-boost immunization (w.p.b.i.), the IgG antibody titer in sera was as high as 1:4,096. IFN-γ and IL-4 levels were significantly different from the control group (P<0.01). This research established a solid foundation for the development of a recombinant adenovirus vaccine against bovine N. caninum.
Deng, Xin-Fa,Zhang, Fuyang,Song, Jun,Chen, Yi-Qing,Jiang, Peng The Korean Astronomical Society 2012 Journal of The Korean Astronomical Society Vol.45 No.3
Using the volume-limited Main galaxy sample constructed from the Sloan Digital Sky Survey Data Release 7 (SDSS DR7), we explore the environmental dependence of the fraction of 'unconventional' galaxies: luminous blue and faint red. It is found that the fraction of faint red increases apparently with increasing local density, and the fraction of luminous blue declines substantially with increasing local density, which shows that there is an environmental dependence for color beyond that for luminosity.
AN IMAGE CODING SCHEME BASED ON THE CLASSIFICATION
Wei, Wang,Jun, Cai De,Guan, Wan Fa 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
Based on the spectral analysis of the discrete cosine transform (DCT), this paper proposes an adaptive image coding scheme, two activity measurements are designed to classify the image subblocks into four classes, various coding techniques are applied to every class, a good visual quality of the reconstructed image at bit rates of 0.2-0.4 bits/pixel (bpp) is achieved.
Gu, Mo-Fa,Su, Yong,Chen, Xin-Lin,He, Wei-Ling,He, Zhen-Yu,Li, Jian-Jun,Chen, Miao-Qiu,Mo, Chuan-Wei,Xu, Qian,Diao, Yuan-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1
Purpose: the study aimed to compare the quality of life (QOL) and radiotherapy complications among Chinese nasopharyngeal carcinoma (NPC) patients at different 3-dimensional conformal radiotherapy (3DCRT) stages adjusting for other variables. Methods: 511 NPC patients at different 3DCRT stages were enrolled. They were interviewed regarding SF-36, complications and socio-demographic variables and cancer- or treatment-related variables. Analysis of covariance (ANCOVA) based on SF-36, complications scores as dependent variables, 3DCRT stages as independent variables, and other variables as covariate were established. Results: The influencing factors of PCS included 3DCRT stages and age group. The influencing factors of MCS included 3DCRT stages and income. Most QOL scores of NPC patients were significantly associated with 3DCRT stage, after accounting for other variables. QOL scores of the patients receiving 3DCRT were the lowest, QOL scores of people after 3DCRT gradually increased. PCS scores of people greater than 5 years after 3DCRT was improved to or even better than the level before 3DCRT. The complications with significantly different scores of patients at different 3DCRT status included xerostomia, throat ache, hypogeusia, caries, hearing loss, snuffles. Conclusions: Clinicians should pay more attention to older NPC patients and patients with lower income. When patients receive 3DCRT, measures should be taken to reduce radiation injury to improve the patients' QOL.
Zhang, Feng-Jun,Zhang, Zhuo,Xie, Fa-Zhi,Xuan, Han,Xia, Hong-Chen,Zhu, Lei,Oh, Won-Chun The Korean Ceramic Society 2014 한국세라믹학회지 Vol.51 No.6
Magnetic-graphene nanosheets have been synthesized via a simple effective chemical precipitation method followed by heat treatment. The composite nanosheets are super paramagnetic at room temperature and can be separated by an external magnetic field. The prepared magnetic-graphene nanosheets were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, and BET surface area analysis. The results demonstrated the successful attachment of iron oxide nanoparticles to graphene nanosheets. It was found that the attached nanoparticles were mainly $Fe_3O_4$. The magnetic-graphene nanosheets showed near complete methyl orange removal within 10 mintues and would be practically usable for methyl orange separation from water.
Physicochemical and Sensory Characterization of Ginger-juice Yogurt during Fermentation
Guo-Hao Yang,Jun-Jun Guan,Jin-Shui Wang,Haicheng Yin,Fa-Dong Qiao,Feng Jia 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6
The primary purpose of this study was to investigate the effects of a ginger (Zingiber ofcinale) juice addition to milk on the physicochemical and sensory properties and consumer acceptability of yogurt during fermentation. Under the presence of ginger juice at 2.0 to 10.0%(v/v), there was a significant influence (p<0.05) on the viability of characteristic microorganisms in yogurt,the pH, titratable acidity (ºSH), viscosity, and hardness of the products. The descriptive data showed significant differences (p<0.05) among the samples for all attributes. The consumer results from external preference mapping indicated that samples with the lower ginger juice (≤4%)were liked over the higher samples (≥6%), with a negative impact on the acceptance by the consumers. Therefore,these findings suggest that 4%(v/v) may be the more appropriate content of ginger juice in the preparation of ginger-juice yogurt.
Aerosol Observation with Raman LIDAR in Beijing, China
Chen-Bo Xie,Jun Zhou,Nobuo Sugimoto,Zi-Fa Wang 한국광학회 2010 Current Optics and Photonics Vol.14 No.3
Aerosol observation with Raman LIDAR in NIES (National Institute for Environmental Studies, Japan) LIDAR network was conducted from 17 April to 12 June 2008 over Beijing, China. The aerosol optical properties derived from Raman LIDAR were compared with the retrieved data from sun photometer and sky radiometer observations in the Aerosol Robotic Network (AERONET). The comparison provided the complete knowledge of aerosol optical and physical properties in Beijing, especially in pollution and Asian dust events. The averaged aerosol optical depth (AOD) at 675 nm was 0.81 and the Angstrom exponent between 440 nm and 675 nm was 0.99 during experiment. The LIDAR derived AOD at 532 nm in the planetary boundary layer (PBL) was 0.48, which implied that half of the total AOD was contributed by the aerosol in PBL. The corresponding averaged LIDAR ratio and total depolarization ratio (TDR) were 48.5sr and 8.1%. The negative correlation between LIDAR ratio and TDR indicated the LIDAR ratio decreased with aerosol size because of the high TDR associated with nonspherical and large aerosols. The typical volume size distribution of the aerosol clearly demonstrated that the coarse mode radius located near 3 μm in dust case, a bi-mode with fine particle centered at 0.2 μm and coarse particle at 2 μm was the characteristic size distribution in the pollution and clean cases. The different size distributions of aerosol resulted in its different optical properties. The retrieved LIDAR ratio and TDR were 41.1sr and 19.5% for a dust event, 53.8sr and 6.6% for a pollution event as well as 57.3sr and 7.2% for a clean event. In conjunction with the observed surface wind field near the LIDAR site, most of the pollution aerosols were produced locally or transported from the southeast of Beijing, whereas the dust aerosols associated with the clean air mass were transported by the northwesterly or southwesterly winds.