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Jangsoo Shim,Heewon Seo,Yohan Choi,Sang-Hwan Hyun,Eunsong Lee,Hakhyun Ka 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s
Cathepsins (CTSs), a family of lysosomal cysteine proteases, and their inhibitors (CSTs) play a critical role in remodeling of the uterine endometrium and placenta for the establishment and maintenance of pregnancy in many animal species including rodents, sheep, cow and pigs. It has been shown that the high rate of pregnancy failure by somatic cell nuclear transfer (SCNT) is associated with abnormal placental development. Our previous study has shown that CST6 is highly expressed in the uterine endometrium from mid to late pregnancy in pigs. In this study, to understand whether appropriate endometrial and placental tissue remodeling occurs in the uterine endometrium from gilts with conceptuses derived from SCNT during pregnancy in pigs, we investigated expression of CST6 in the uterine endometrium. Uterine endometrial tissues were obtained from gilts that carried SCNT-derived normal conceptuses (NT-No) and abnormal conceptuses (NT-Ab), and from gilts carrying conceptuses from natural mating (Non-NT) on D114 of pregnancy. Immunoblot analysis showed that CST6 protein levels in the endometrial tissues of gilts carrying NT-No were lower than those of gilts carrying Non-NT. The levels of CST6 protein in the endometrial tissues of gilts carrying NT-Ab decreased even more than those of gilts carrying NT-No. These results indicate that decreased expression of CST6 in the endometrium with NT-No and NT-Ab reflects inappropriate endometrial tissue remodeling and pregnancy failure of pigs with SCNT derived conceptuses and that CST6 plays an important role for the maintenance of pregnancy in pigs. * This work was supported by the Next Generation BioGreen 21 program (#PJ007997), RDA, Republic of Korea.
Analysis of LGMN and CST6 at the Maternal-Fetal Interface during Pregnancy in Pigs
Jangsoo Shim,Heewon Seo,Yohan Choi,Mingoo Kim,Hakhyun Ka 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
Proteases and their inhibitors are involved in the process of pregnancy by remodeling uterine endometrium and placenta in many mammals. During placentation, proteases and their inhibitors contribute to formation of epitheliochorial type placentation in pigs. Our previous study showed that LGMN and CST6 were expressed in the uterine endometrium and localized mainly to glandular epithelial cells (GE) and chorionic membrane (CM) during mid to late pregnancy. In this study, we investigated expression of LGMN and CST6 in the uterine endometrium and fetal membrane during pregnancy in pigs. Uterine endometrial tissue samples and fetal membrane samples were collected from D30, D60, D90, and D114 of pregnancy. Real-time RT-PCR analysis showed that both LGMN and CST6 mRNAs were detected in the uterine endometrium and fetal membrane in all samples with higher levels during mid to late stage of pregnancy. Analysis by immunoblotting revealed that LGMN protein was present in the porcine uterine endometrium and fetal membrane. Based on the placental and endometrial distribution of proteases and their inhibitors, we examined LGMN mRNA and LGMN protein expression in the neonatal pigs. In situ hybridization analysis using the intestine from D90 of piglet revealed that LGMN mRNA was highly expressed in the absorptive epithelium of the intestinal villi. Immunohistochemical experiments demonstrated that LGMN protein was localized to epithelial villi. These results suggest a possible role of LGMN in modification of proteins that are transported through the fetal membrane from the uterine for successful transport and utilization in the fetus.
Low-complexity 1D-convolutional Neural Network for Super Resolution
Jangsoo Park,Jongsuk Lee,Donggyu Sim 대한전자공학회 2019 IEIE Transactions on Smart Processing & Computing Vol.8 No.6
This paper proposes a method for accelerating deep learning based super-resolution technology. In order to alleviate the complexity of the deep learning based super-resolution technology, the proposed method extracts the horizontal and vertical high-frequency signals separately using one-dimensional filters. Then, the final super-resolution image is obtained by the proposed network from the horizontal and vertical high-frequency signals and the low-resolution input image. The proposed method requires a low computational complexity by using only onedimensional filters due to a smaller number of weights. The proposed method in the high-resolution image restoration experiment shows that the average visual quality in PSNR and SSIM is comparable to ones of VDSR. However, the average speed performance is accelerated by 86.57%.
박장수(Jangsoo Park),이재원(Jayone Lee),이성범(Seong Beom Lee),김흥섭(Heungseob Kim) 한국자동차공학회 2010 한국자동차공학회 부문종합 학술대회 Vol.2010 No.5
The purpose of this paper is to compare with estimation of equivalent fatigue load in time domain and frequency domain. The fatigue analysis for random loads is discussed in several papers. In general, Equivalent fatigue load in time-domain is estimated by rainflow cycle counting. In case of frequency domain, it can estimate through probability density function of Dirlik`s method that directly use power spectrum density of stress. Probability density function of Dirlik`s method is function of spectral moment only. For verification of performance with two methods, simulations is performed with general random signal and FEM analysis.
류장수(Jangsoo Ryu),박성익(Sungik Park),조장식(Jangsik Cho),김종한(Jonghan Kim),곽소희(Sohui Kwak) 한국고용노사관계학회 2013 産業關係硏究 Vol.23 No.3
지방의 우수 인재들이 수도권으로 이동하는 중요한 이유는 괜찮은 일자리(decent job)가 수도권에 집중해 있거나, 수도권 인재를 선호하기 때문이라는 연구가 다수 있었다. 공공기관의 일자리는 대기업과 함께 괜찮은 일자리를 형성하는 한 축으로 작용하고 있다. 본 논문은 이 점에 착안하여 과연 지난 5년 동안 공공기관들이 지방 인재를 어느 정도로 채용하였고, 그 채용규모를 결정하는 요인들이 무엇인지를 분석하는 데 연구 목적을 두었다. 이러한 분석을 통해 보다 효과적인 지방 인재 활용의 활성화 및 지역 발전전략을 마련할 수 있을 것이다. 본 연구의 주요 결과는 다음과 같다. 먼저, 지난 5년간 공공기관의 신규채용자 중 47.0%가 지방 인재이고 나머지 53.0%는 수도권인재였다. 정부의 권고 수치인 지방 인재 30% 채용을 달성하지 못한 공공기관 비중은 약 50%에 이른다. 그리고 회귀분석 결과, 평균 보수액이 높은 공공기관일수록 통계적으로 유의하게 지방 인재의 채용 비율은 낮았다. 이러한 현상은 지방의 우수 고졸자들이 수도권 대학으로 유출하는 원인의 하나로 작용했을 것이고, 그 결과 지방에는 우수 인재가 크게 부족한 실정이다. 공공기관의 지방 인재 채용할당제를 통해 지방대학에 우수 인재가 입학하고 이들 중 일부가 지방에 남아 지역 발전을 선도할 수 있도록 해야 할 필요가 있다. According to the previous research, major reason why excellent graduates from local high schools and universities move to Seoul metropolitan area(=SMA) is either their preference for universities or a decent job in SMA. Jobs in public institutions as well as in the large companies constitute the mainstays of decent jobs. Based on this perception, we investigate how many provincial graduates have been employed in public institutions for the past five years and what determine the size of employment. This research will help invigorate the effective utilization of provincial graduates and establish a strategy for regional development. Our main results are as follows. For the past five years, provincial graduates accounted for 47.0 percent of the new employees in public institutions while SMA graduates did 53.0 percent. The half of public institutions have employed provincial graduates less than 30 percent out of total new employees, which is the government’s recommendation level. In the regression analysis, it is revealed that the share of employing provincial graduates in public institutions located in SMA is significantly lower than that of public institutions located in other provinces. It is also shown that the institutions which pay higher salary have a tendency to employ less provincial graduates.