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김현걸 ( Hyun Guell Kim ),이승철 ( Seung Cheol Lee ) 한국도서학회 2014 韓國島嶼硏究 Vol.26 No.2
In order to study the movement of a pearl`s body, the Tongyeong pearl powder (insoluble=pearl) and manufactured powder (soluble-pearl, solubilized in water) were used as samples. The body of a pearl contains calcuim compounds and pharmacologically activated chonchiolin. This research evaluated the water solubilization level of proteins and the solubility of pearl powder using artificial gastric juice and intestinal juice. The analysis of the sugar content showed that no soluble or insoluble-pearl was found. This showed that carbohydrates are not the main components of the pearl. Also, in order to evaluate the water solubility level of chonchiolin, which is the only protein of the pearl, a protein quantification experiment was conducted. The result showed that no protein was found in the insoluble-pearl, which confirmed that chonchiolin was insoluble. In the soluble-pearl, protein consisted of 0.12%, and the effects of protein`s acid hydrolysis in acetic acid during the water solubilization process was either none or very weak. Such results showed that the development of chonchiolin water solubilization using protease is necessary. From the results that showed 22.7% of solubility (37℃ body temperature) at artificial gastric juice and 1.7% at artificial intestinal juice, the insoluble-peral is expected to dissolve into a small amount within the stomach(acidic environment), and rarely dissolve in the small intestine absorption area. Also, 75% of the dosage is expected to be excreted from the body. In order to find out the exact absorption level within the body of a pearl, more research on the vitro level and continuous biological tests is required. The solubility of soluble-pearl manufactured by using insoluble-pearl powder is 32.9%, which is 1.4 fold higher than insoluble-pearl. It is 17.6% at the artificial intestinal juice and it`s solubility is 10 fold higher than insoluble-pearl. The calcium carbonate inside the pearl is assumed to convert into calcium acetate, which is soluble to a certain degree during the water solubilization process. However, considering the fact that the solubility value is not as much as expected, from now on, improving the condition of the water solubilization process such as increasing reaction temperature is needed.
12주간 복합운동이 중년여성의 신체조성 및 골밀도에 미치는 영향
김현걸(Kim, Hyun-Guell),최승욱(Choi, Seung-Wook) 한국체육과학회 2011 한국체육과학회지 Vol.20 No.5
The purpose of this study was to identify the effects of 12 weeks combined exercise on body composition, bone mineral density on middle-age woman. For this purpose, 14 middle-age woman were randomly assigned to four different groups of four subjects per each(group of exercise alone, and a group of control) and weight, %Body fat, lean, bone mineral contect, bone mineral density(head, arms, trunk, ribs, pelvis, spine & Total) were measured. Analysis results of subjects after the twelve-weeks program are summarized in the following. 1) After the twelve-week program, body composition was significantly decreased in the groups of exercise with control. 2) After the twelve-week program, head, arms, trunk, ribs, pelvis, spine & Total BMD was significantly decreased in the groups of exercise with control but legs BMD was significantly improved in the exercise group. 12-week combined exercise program consisting help maintaining BMD and preventing bone mass loss. These positive effects help to lower the risk of osteoporosis for middle age woman.
12주간의 복합운동이 고령여성 경계성고혈압 환자의 혈압, 혈청지질 및 혈당에 미치는 영향
권정현(Kwon Jeong-Hyun),김현걸(Kim Hyun-Guell) 한국체육과학회 2007 한국체육과학회지 Vol.16 No.3
The purpose of the study was to find out the effect on the change in blood pressure, serum lipids and glucose of combined exercise(aerobic dance, resistance exercise and stretching) of the older women(borderline hypertensive patients) during 12 weeks. The combined exercise was consist of aerobic dance, weight, dumbbell and elastic band with the intensity of RPE 11-13, 50%-60% of HRR% and 40%-50% of IRM for 75 minute in a time by three day a week. The conclusions obtained were as follows; 1. HDL-C was increased by 3.76% in comparison preㆍpost combined exercise, it wasn't statistically significant. 2. LDL-C was increased by 0.84% in comparison preㆍpost combined exercise, it wasn't statistically significant. 3. TG was increased by 14.16% in comparison preㆍpost combined exercise, it wasn't statistically significant. 4. TC was decreased by -1.63% in comparison preㆍpost combined exercise, it wasn't statistically significant. 5. SYS was decreased by -11.36% in comparison preㆍpost combined exercise, it was statistically significant(p<.05). 6. DIA was decreased by -4.80% in comparison preㆍpost combined exercise, it wasn't statistically significant. 7. Glucose was increased by 3.63% in comparison preㆍpost combined exercise, it wasn't statistically significant.
Purification and Anticoagulant Activity of a Fucoidan from Korean Undaria pinnatifida Sporophyll
Kim , Woo-Jung,Kim, Sung-Min,Kim, Hyun-Guell,Oh, Hye-Rim,Lee, Kyung-Bok,Lee, Yoo-Kyung,Park, Yong-Il The Korean Society of Phycology 2007 ALGAE Vol.22 No.3
Crude fucoidan was extracted from the sporophyll of Korean Undaria pinnatifida collected at a coastal area ofWando, Korea, mainly by dilute acid extraction, ethanol precipitation, CaCU Precipitation, with an yield of approxi-mately 3.9% in mass. It was further purified by DEAE-cellulose column chromatography and its chemical composi-don and in vitro anticoagulant activity was determined. The average molecular mass of the purified fucoidan wasestimated about 2.1 x 103 kDa by size-fractionation HPLC and it consisted of neutral sugar (52.34% in mass), uronicacid (26.2%), and sulfate esters (7.4%). From the HPAEC-PAD analysis, the monosaccharide composition of thepurified fucoidan was shown to be fucose, galactose, xylose, and mannose, with a molar ratio of 1, 0.2, 0.02, 0.15,respectively, demonstrating that major monosacd-iande was fucose (72.3% in mol percentage) and other sugars,xylose (1.5%), galactose (14.6%), and mannose (10.9%) were present as minor component. The results suggested thatthis fucoidan is a sulfated, U-type fucoidan. The activated partial thrombloplastin time (APTT) assay of the purifiedfucoidan showed that the purified fucoidan elicited anticoagulant activity in a dose-dependent manner. Five jUg ofsporophyll fucoidan delayed the blood clotting time up to 5 times than untreated control and also up to 1.5 timesthan the same amount of the commercial fucoidan, respectively. Although it is preliminary, these results suggestthat the fucoidan of Korean Undaria vinnatifida sporophyll would be promising candidates for the development ofan anticoaeulant.