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In Vitro Biological Characterization of DCUN1D5 in DNA Damage Response
Guo, Wei,Li, Guo-Jun,Xu, Hong-Bo,Xie, Jie-Shi,Shi, Tai-Ping,Zhang, Sheng-Zhong,Chen, Xiao-Hong,Huang, Zhi-Gang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Background: Novel prognostic biomarkers or therapeutic molecular targets for laryngeal squamous cell carcinoma (LSCC) are an urgent priority. We here sought to identify multiple novel LSCC-associated genes. Methods: Using high-density microarray expression profiling, we identified multiple genes that were significantly altered between human LSCCs and paired normal tissues. Potential oncogenic functions of one such gene, DCUN1D5, were further characterized in vitro. Results: Our results demonstrated that DCUN1D5 was highly expressed in LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular migration, 67.5% increased invasive capacity, and 2.6-fold increased proliferation. Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. Conclusion: Our data suggest that DCUN1D5 in vitro might have vital roles in DNA damage response, but further studies are warranted to assess its significance in vivo.
Guo, Yan-Wu,Guo, Hui-Li,Li, Xing,Huang, Li-Li,Zhang, Bo-Ning,Pang, Xiao-Bin,Liu, Ben-Ye,Ma, Lan-Qing,Wang, Hong 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
In our recent work (Ma et al., in Planta 229(3):457-469, 2009a and 229(4):1077-1086, 2009b), two three-intron type III PKS genes, PcPKS1 and PcPKS2, were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is a three-intron chalcone synthase (CHS) gene, and PcPKS2 is found to be a three-intron benzalacetone synthase (BAS) gene. The regular CHS encoded by a single intron gene have not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3) and a stilbene synthase (STS) gene with three-intron (PcPKS5) were isolated and characterized by functional and phylogenetic analyses. In comparison with PcPKS1, a bifunctional enzyme with both CHS and BAS activity, the enzymatic product of recombinant PcPKS3 was naringenin, bis-noryangonin (BNY) and 4-coumaroyltriacetic acid lactone (CTAL) occurred as side products. The PcPKS5 synthesized resveratrol and a trace amount of naringenin from p-coumaroyl-CoA. To our knowledge, PcPKS5 is the first reported three-intron STS gene in flowering plants. In this work, we speculated that this involved a possible evolutionary route of plant-specific type III PKS superfamily in P. cuspidatum.
Guo-Bo Xu,Tao Yang,Jin-ku Bao,Dong-Mei Fang,Guo-You Li 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5
Isochaetomium A2 (1), a new bis(naphthodihydropyran-4-one), along with chaetochromins A (2) and B(3), was isolated from the solid-state fermented rice cultureof Chaetomium microcephalum. The structure of compound1 was elucidated on the basis of 1D and 2D NMRspectral data, and the relative configuration was confirmedby CD spectrum. Compounds 1–3 possessed significantantimicrobial activity against Escherichia coli 1.044,Staphylococcus aureus 1.252, and Bacillus subtilis 1.079. Moreover, compounds 1–3 showed obvious inhibitoryeffects on mouse spleen cell proliferation with successiveIC50 values of 0.52, 0.19, and 0.24 lM.
Fu, Bo,Ren, Liang,Liu, Di,Ma, Jian-Zhang,An, Tie-Zhu,Yang, Xiu-Qin,Ma, Hong,Zhang, Dong-Jie,Guo, Zhen-Hua,Guo, Yun-Yun,Zhu, Meng,Bai, Jing Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.12
The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogenase (G6PDH) activity have shown superior quality than BCB negative (-) oocytes with high G6PDH activity, the use of a BCB staining test before IVM is still controversial. This study aimed to shed more light on the subcellular characteristics of porcine oocytes after selection using BCB staining. We assessed germinal vesicle chromatin configuration, cortical granule (CG) migration, mitochondrial distribution, the levels of acetylated lysine 9 of histone H3 (AcH3K9) and nuclear apoptosis features to investigate the correlation between G6PDH activity and these developmentally related features. A pattern of chromatin surrounding the nucleoli was seen in 53.0% of BCB+ oocytes and 77.6% of BCB+ oocytes showed peripherally distributed CGs. After IVM, 48.7% of BCB+ oocytes had a diffused mitochondrial distribution pattern. However, there were no significant differences in the levels of AcH3K9 in the nuclei of blastocysts derived from BCB+ and BCB- oocytes; at the same time, we observed a similar incidence of apoptosis in the BCB+ and control groups. Although this study indicated that G6PDH activity in porcine oocytes was correlated with several subcellular characteristics such as germinal vesicle chromatin configuration, CG migration and mitochondrial distribution, other features such as AcH3K9 level and nuclear apoptotic features were not associated with G6PDH activity and did not validate the BCB staining test. In using this test for selecting porcine oocytes, subcellular characteristics such as the AcH3K9 level and apoptotic nuclear features should also be considered. Adding histone deacetylase inhibitors or apoptosis inhibitors into the culture medium used might improve the efficiency of IVM of BCB+ oocytes.
Zhong bo Guo,Jong Ho Lee 한국유통과학회 2016 KODISA ICBE (International Conference on Business Vol.2016 No.-
The legacies, given to us by these inspirators, innovators, insignificant individuals of history all began with questions encapsulated in their mind like a sentence, "can I make this better?" Betterment has driven innovation and re-made our questionable imagination into significant life improvement unit. Thus, with my passion for the convenience of travelling and my love for enjoying the surrogate driving experience, revolutionizing the taxi-call industry had been the primary goal of my research topic. Kicking off with meticulous and comprehensive effort. The purpose of this article is to examine the effects of perceived value, satisfaction, continued use intention of e-service Quality of taxi-booking apps. As known by all, the smart phone industry had uprised and uproar since the introduction of game changing phone in to the market, the Apple Iphone 3G, phones and business related industries had utilized such digital telecommunication platform to wide-stretched their business.
Effects of Taxi-Booking Apps of E-Service Quality on Use Intention in China
Zhong-Bo, Guo,Uk-Yeol Park,Jong-Ho Lee 한국유통과학회 2018 The Journal of Industrial Distribution & Business( Vol.9 No.4
Purpose - The purpose of this article is to examine the effects of perceived value, satisfaction, and continued use intention of e-service Quality of taxi-booking apps. Research design, data, and methodology - The questionnaire was created to examine every relevant variables to practical and theoretical implications. The pilot survey was conducted for 15 days from April 7th to April 21st in 2016. Their total numbers were 354 surveys. But 330 copies were used for the analysis except 24 of them. To make a more effective analysis, several analysis tools and analysis programmes were used, such as IBM SPSS and AMOS. Results - The results are as follows. First, all variables of e-service quality except safety make influences on perceived value. Second, e-service quality except safety makes influences on satisfaction. Third, perceived value makes influence on satisfaction and on intention of continued use. Fourth, satisfaction makes influence on intention of continued use. Conclusions - First, 5 variables are very limited. more valuable variables are need to test with this model. Second, this study was conducted only in China. So there may be possible to the representatives. To use this model for better analysis and application abroad, more characteristics and strategic factors should be considered like the local culture of a particular country, attributes for different environment etc.
Xu, Guo-Bo,Yang, Tao,Bao, Jin-Ku,Fang, Dong-Mei,Li, Guo-You 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5
Isochaetomium $A_2$ (1), a new bis(naphthodihydropyran-4-one), along with chaetochromins A (2) and B (3), was isolated from the solid-state fermented rice culture of Chaetomium microcephalum. The structure of compound 1 was elucidated on the basis of 1D and 2D NMR spectral data, and the relative configuration was confirmed by CD spectrum. Compounds 1-3 possessed significant antimicrobial activity against Escherichia coli 1.044, Staphylococcus aureus 1.252, and Bacillus subtilis 1.079. Moreover, compounds 1-3 showed obvious inhibitory effects on mouse spleen cell proliferation with successive $IC_{50}$ values of 0.52, 0.19, and $0.24{\mu}M$.
An Exploratory Study on the Key Variables of the Characteristics of Taxi-Calling Apps
Zhong bo Guo,Jong Ho Lee 한국유통과학회 2016 KODISA ICBE (International Conference on Business Vol.2016 No.-
Within the 21st century, life has so much changes amazingly. Our lives had become better than before, unprecedently marching towards the betterment of society as new creation meets innovation, sparking a new experience. Unvaried to other elements in life, the development of technology had impacted and redefined how business runs, raging more profits than ever before, yet skyrocketing the sales figures to new records. The purpose of this article is to examine the key factors of the characteristics of taxi-calling apps because of insufficient researches on them with development of high technologies and wide-spread smartphones' apps all over the world. And even more it is considered to have useful skills on smartphones' apps. They will make diversified influences on human lives. Also to have knowledge and skill on them is considered as a kind of competition edge.
SINGLE MACHINE GROUP SCHEDULING UNDER DECREASING LINEAR DETERIORATION
Wang, Ji-Bo,Guo, Ai-Xia,Shan, Feng,Jiang, Bo,Wang, Li-Yan 한국전산응용수학회 2007 Journal of applied mathematics & informatics Vol.24 No.1
This paper considers single machine scheduling problems with group technology (GT) and deteriorating jobs. A sequence independent setup is required to process a job from a different group and jobs in each group are processed together. We consider the case of jobs whose processing times are a decreasing function of their starting time. The objectives of scheduling problems are to minimize the makespan and the total completion time, respectively. We also provide polynomial time algorithms to solve these problems.