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Development of a meso-galactarate Production Platform in Engineered Yeast
Deokyeol JEONG,Heeyoung PARK,Sujeong PARK,Suhyeung KIM,Byeong-Kwan JANG,Je Min LEE,Soo Rin KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
meso-galactarate, a platform chemical of bioplastic, is converted by some pathogenic bacterial uronate dehydrogenase (udh) from D-galacturonic acid, which is abundantly present in pectin-rich biomass. Although microbial meso-galactarate production has been mostly studied based on filamentous fungi, an optimal platform has not been developed for yeast Saccharomyces cerevisiae, despite its large capacities such as high acid tolerance and rapid growth. The purpose of present study is to develop an S. cerevisiae strain that can efficiently convert meso-galactarate. Three different udh genes and overexpression of one of them were completely introduced by Cas9-genome editing in S. cerevisiae, allowed the conversion of meso-galactarate completely without specific transporter of D-galacturonic acid. Moreover, the additional expression of the dicarboxylic acid transporter gene in the meso-galactarate producing strain showed higher meso-galactarate production efficiency. The final engineered strain showed a high titer of meso-galactarate from citrus peel waste hydrolysate. Through this study, we can suggest that the possibility as a platform host to utilize pectin-rich biomass.
The effects of feed materials on the nutrient composition of Protaetia brevitarsis larvae
Deokyeol Jeong,NamgyongMIN,김영부,Soo Rin Kim,Ohseok KWON 한국곤충학회 2020 Entomological Research Vol.50 No.1
To find out the effect of feed materials on the nutrient composition of Protaetia brevitarsis larvae (Pbl), three different feed materials were prepared and fed to the Pbl populations. After the Pbl population reached the third instar stage, they were boiled and dried for the nutrient analysis. The results showed that the nutrient composition of Pbl varied in response to the feed materials. Therefore, it is critical to set a feed material standard to guarantee the efficacy of Pbl medicinal property.
발효식품의 품질을 저해하는 부패효모의 발생원인과 검출 및 제어방법
정덕열(Deokyeol Jeong),박희영(Heeyoung Park),배은영(Eun-Yeong Bae),설민경(Min-Kyeong Seol),주예빈(Ye-Bin Ju),김종석(Jong-Seok Kim),장병관(Byeong-Kwan Jang),Sultanov Akhmadjon,김병오(Byung-Oh Kim),조영제(Young-Je Cho),김수린(Soo Ri 한국생물공학회 2020 KSBB Journal Vol.35 No.1
It is not commonly recognized that yeast is responsible for food spoilage as well as food fermentation. Fermented beverages and foods have several factors that inhibit bacterial growth such as high contents of organic acids, sugars, salts, and/or alcohol; however, some yeast species can tolerate these stress factors and cause food spoilage. Specifically, during wine and beer fermentation, if some contaminated spoilage yeast species grow dominantly over fermentation starter yeast, the products would have undesirable flavor. More importantly, during storage of fermented kimchi and soy sauce, some spoilage yeast species slowly grow on the surface of the products and produce gases, which limits product shelf life and exportability. In this review, spoilage yeast species commonly found in fermented foods are introduced. Next, contamination routes, detection methods, and control methods of the spoilage yeast will be described.
일관수송용 T-11형 및 T-12형 파렛트에 적합한 연안용 화물컨테이너(8 & 12 피트) 공용 표준치수 산정에 관한 연구
김덕열 ( Deokyeol Kim ),서병륜 ( Byongyoon Suh ),송상화 ( Sanghwa Song ) 한국로지스틱스학회 2017 로지스틱스연구 Vol.25 No.3
본 연구는 일관수송용 파렛트(T-11, T-12 형)에 적합한 연안용 화물컨테이너(8, 12 피트)의 공용 배수 치수를 개발하는 것이며, 물류 모듈체계의 하나인 파렛트 최대 평면치수 1,140 x 1,140mm와 1,240 x 1,040mm를 기준으로 배가법과 승산법을 적용하여 컨테이너의 공용 배수치수를 선정하였다. 첫째, 화물컨테이너의 구조는 측면도어 개방형으로 최소 안쪽 너비는 2,280mm로 고정하고 길이는 CAPE 시뮬레이션을 이용하여 조정하였다. 둘째, 최대 평면 치수를 8피트에 4개, 12피트에 6개가 적재되도록 2 내지 3배로 배가한 다음에 상 · 하역 작업의 여유치수를 추가하여 새로운 1차적 치수를 도출하였다. 셋째, 1차로 도출한 치수 중에서 가장 큰 치수를 공용 배수 치수로 선정하고, 컨테이너에 파렛트 4개 또는 6개의 적재방법과 효율을 분석하여 최적화 여부를 시뮬레이션하고 ISO 및 KS 규격에 부합되는 가를 최종 확인하였다. 따라서, 두 종류 파렛트에 대한 컨테이너 공용 배수치수는 최소 내부 너비가 2,280mm, 내부 길이는 8피트가 2,580mm, 12피가 3,580mm로 선정되었다. 공용 배수치수는 파렛트 적재효율이 8피트의 경우 T-11형이 83.9%, T-12형이 83.2%, 혼적 시에 83.5%, 평균 적재효율은 83.53%이며, 12피트는 T-11형이 88.9%, T-12형이 88.2%, 혼적하는 경우 88.4%, 평균 적재효율은 88.5%이다. 결론적으로 공용 배수치수는 파렛트 적재효율이 모두 83% 이상으로 되어 비교적 우수한 것으로 판단되며, 평균 적재효율은 12피트가 8피트 보다 약 5% 더 높은 것으로 나타났다. The purpose of this study is to develop common size for coastal freight containers (8, 12 feet) suitable for through transit pallets (T-11, T-12 type). The common doubling size of the container were selected by applying doubling method and multiplication method based on the plan view size(pvs) of 1,140 x 1,140 mm and 1,240 x 1,040 mm, which is one of the logistics module systems. First, the structure of the freight container is the side door open type, the minimum inside width size is fixed to 2,280 mm, and the inside length size were adjusted by CAPE simulation program. Second, a new primary dimension was derived by doubling the plan view size of the four pallets to 8 feet, six pallets to 12 feet, and then adding the clearance of the material handling loading operations. Third, the common doubling size was selected the largest size among the first derived sizes, simulated the optimization by analyzing the loading method and efficiency of four or six pallets in the container, and finally confirmed whether it compliance with the ISO and KS standards. Therefore, the inside width of both containers is 2,280mm, the inside length size of 8 feet is 2,530mm and 12 feet container is 3,580mm. The load efficiency of 8 feet container, T-11 type is 83.9% and T-12 type is 83.2%, mixed with T-11 and T-12 type is 83.5 %, average is 83.53%, and in case of 12 feet, the load efficiency of T-11 type is 89.9%, T-12 type is 88.2%, mixed with T-11 and T-12 type is 88.4% average load efficiency is 88.5%. As a result, it is judged that the common doubling sizes are comparatively excellent because the loading efficiency of the pallet is over 83%, and the average loading efficiency of 12 feet container is more 5% higher than 8 feet.
Expression of Heterologous Bacterial CO₂-fixation Pathway in Yeast
Sujeong PARK,Deokyeol JEONG,Suhyeung KIM,Bonbin GU,Soo Rin KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Unpredictable climate change caused by global warming is threatening humanity and the ecosystem. To solve this problem, more than 110 countries and international organizations have agreed to reduce carbon dioxide (CO₂) emissions. Recently various industrial processes such as bioethanol and chemical production rely on yeast fermentation. In the yeast fermentation process, CO₂ emission into atmosphere is unavoidable. Therefore, expressing CO₂ fixation pathway in yeast would decrease the CO₂ emission during the process. The purpose of this study is to develop a S. cerevisiae strain that can utilize CO₂ generated during the fermentation. First, we constructed xylose oxidoreductase pathway to produce the precursor of CO₂ fixation pathway. Then, heterologous genes related to CO₂ fixation pathway are introduced by the CRISPR/Cas9 genome editing technology. We confirmed the soluble expression of ribulose-1,5-bisphosphate carboxylase/oxygenase. Moreover, we observed increased ethanol yield through the fermentation. These results suggest that expressing CO₂ fixation pathway in S. cerevisiae could be a strategy to alleviate the global warming.