http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Chawon Yun ),( Sou Hyun Kim ),( Doyoung Kwon ),( Mi Ran Byun ),( Ki Wung Chung ),( Jaewon Lee ),( Young-suk Jung ) 한국응용약물학회 2024 Biomolecules & Therapeutics(구 응용약물학회지) Vol.32 No.1
Non-alcoholic fatty liver disease (NAFLD) is characterized by excessive accumulation of fat in the liver, and there is a global increase in its incidence owing to changes in lifestyle and diet. Recent findings suggest that p53 is involved in the development of non-alcoholic fatty liver disease; however, the association between p53 expression and the disease remains unclear. Doxorubicin, an anticancer agent, increases the expression of p53. Therefore, this study aimed to investigate the role of doxorubicin-induced p53 upregulation in free fatty acid (FFA)-induced intracellular lipid accumulation. HepG2 cells were pretreated with 0.5 μg/mL of doxorubicin for 12 h, followed by treatment with FFA (0.5 mM) for 24 h to induce steatosis. Doxorubicin pretreatment upregulated p53 expression and downregulated the expression of endoplasmic reticulum stress- and lipid synthesis-associated genes in the FFA -treated HepG2 cells. Additionally, doxorubicin treatment upregulated the expression of AMP-activated protein kinase, a key modulator of lipid metabolism. Notably, siRNA-targeted p53 knockdown reversed the effects of doxorubicin in HepG2 cells. Moreover, doxorubicin treatment suppressed FFA -induced lipid accumulation in HepG2 spheroids. Conclusively, these results suggest that doxorubicin possesses potential application for the regulation of lipid metabolism by enhance the expression of p53 an in vitro NAFLD model.
Orbit Error Correction on the High Energy Beam Transport Line at the KHIMA Accelerator System
Chawon Park,Heejoong Yim,Garam Hahn,Dong Hyun An 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.69 No.6
For the purpose of treatment of various cancers and medical research, a synchrotron based medical machine has been developed under the Korea Heavy Ion Medical Accelerator (KHIMA) project and is scheduled for use to treat patient at the beginning of 2018. The KHIMA synchrotron is designed to accelerate and extract carbon ion (proton) beams with various energies from 110 to 430 MeV/u (60 to 230 MeV). Studies on the lattice design and beam optics for the High Energy Beam Transport (HEBT) line at the KHIMA accelerator system have been carried out using the WinAgile and the MAD-X codes. Because magnetic field errors and misalignments introduce deviations from the design parameters, these error sources should be treated explicitly, and the sensitivity of the machine’s lattice to different individual error sources should be considered. Various types of errors, both static and dynamic, have been taken into account and have been consequentially corrected with a dedicated correction algorithm by using the MAD-X program. Based on the error analysis, the optimized correction setup is decided, and the specifications for the correcting magnets of the HEBT lines are determined.
( Chawon Yun ),( Hyun Jae Lee ),( Choong Jae Lee ) 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.6
In this study, we investigated whether eriodictyol exerts an effect on the production and gene expression of MUC5AC mucin in human pulmonary epithelial NCI-H292 cells. The cells were pretreated with eriodictyol for 30 min and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 h. The effect of eriodictyol on PMA-induced nuclear factor kappa B (NF-κB) signaling pathway was also investigated. Eriodictyol suppressed the MUC5AC mucin production and gene expression induced by PMA via suppression of inhibitory kappa Bα degradation and NF-κB p65 nuclear translocation. These results suggest that eriodictyol inhibits mucin gene expression and production in human airway epithelial cells via regulation of the NF-κB signaling pathway.
Small Molecule Drug Candidates for Managing the Clinical Symptoms of COVID-19: a Narrative Review
( Chawon Yun ),( Hyun Jae Lee ),( Choong Jae Lee ) 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.6
Towards the end of 2019, an atypical acute respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was identified in Wuhan, China and subsequently named Coronavirus disease 2019 (COVID-19). The rapid dissemination of COVID-19 has provoked a global crisis in public health. COVID-19 has been reported to cause sepsis, severe infections in the respiratory tract, multiple organ failure, and pulmonary fibrosis, all of which might induce mortality. Although several vaccines for COVID-19 are currently being administered worldwide, the COVID-19 pandemic is not yet effectively under control. Therefore, novel therapeutic agents to eradicate the cause of the disease and/or manage the clinical symptoms of COVID-19 should be developed to effectively regulate the current pandemic. In this review, we discuss the possibility of managing the clinical symptoms of COVID-19 using natural products derived from medicinal plants used for controlling pulmonary inflammatory diseases in folk medicine. Diverse natural products have been reported to exert potential antiviral effects in vitro by affecting viral replication, entry into host cells, assembly in host cells, and release. However, the in vivo antiviral effects and clinical antiviral efficacies of these natural products against SARS-CoV-2 have not been successfully proven to date. Thus, these properties need to be elucidated through further investigations, including randomized clinical trials, in order to develop optimal and ideal therapeutic candidates for COVID-19.
Effect of Titanium on Structure of Sodium Borophosphate Glasses
Donghwan Kim,Chawon Hwang,Donggun Gwoo,Taehee Kim,Namjin Kim,류봉기 대한금속·재료학회 2011 ELECTRONIC MATERIALS LETTERS Vol.7 No.4
Glasses in the system 30P₂O_5-30B₂O₃-40Na₂O-xTiO₂ (0 ≤ x ≤ 25 mol %) have been prepared by the meltquenching technique. Increasing the TiO₂ content of the glass structure results in a decrease in the molar volume and an increase in the glass transition/softening temperatures. Structural changes were studied by Raman spectroscopy, and 11 B and 31P solid-state NMR. The observed changes in the spectra and the properties of the studied glasses can be ascribed to several reasons, such as the addition of TiO₂ that results in the formation of distorted Ti octahedral linked to pyrophosphate unit through P–O–Ti bonds. In these glass series,the overall glass network was enhanced by TiO₂ addition, although a depolymerization in the original P–O–P network occurred.
Effects of Substitution of K2O for Na2O on the Bioactivity of CaO-Na2O-SiO2-P2O5 Glasses
Taehee Kim,Chawon Hwang,Donggun Gwoo,Hoyyul Park,류봉기 대한금속·재료학회 2012 ELECTRONIC MATERIALS LETTERS Vol.8 No.5
The compositional dependences of bioactivity, thermal properties, atomic structure, and surface morphology have been investigated in the CaO-Na2O-SiO2-P2O5 system; this system is known as a bioglass. 45S5 Bioglass®is known to be a general and highly bioactive material. However, the bioactivity of this glassy material is expected to be improved by modifying the alkali-metal composition. Thermal properties, density, and molar volume were measured to investigate the structural packing. FT-IR spectra and X-ray diffraction were used to confirm the structures of these glasses. The morphology was examined using field emission electron microscopy,and the formation of a Ca-P layer was studied using an energy-dispersive system. This study shows that the tendency to form a calcium phosphate layer is increased with the substitution of K2O for Na2O.
Synthesis and Characterization of CdS Nanocrystals in a Novel Phosphate Glass
Donghwan Kim,Chawon Hwang,Donggun Gwoo,Taehee Kim,Youngseok Kim,Namjin Kim,류봉기 대한금속·재료학회 2011 ELECTRONIC MATERIALS LETTERS Vol.7 No.4
Zinc-borophosphate glasses doped with various concentrations (≤ 5 wt. %) of cadmium sulfide (CdS) were synthesized. Nanocrystals of CdS were developed in the glass matrices at different concentrations, with emphasis on the quantum confinement effect. The effects of CdS content on the optical and various other properties were investigated. The optical characterization of the glasses was carried out using UV-Visible spectrophotometry. There was a red shift in the optical cut-off with increasing CdS content in the glass. The band gap of the CdS nanocrystals doped in the glass matrix ranged from 3.5 eV to 4.2 eV. The structural characterization,which was carried out using X-ray diffraction, shows the CdS to have hexagonal crystal structure. The average size and shape of the nanocrystals doped in the glass matrix were determined using transmission electron microscopy (TEM). The particle sizes of the doped CdS crystals were in the range of 3 nm to 6 nm for 2 wt. % CdS, and 15 nm to 20 nm for 5 wt. % CdS.
Wang, Jin-Hee,Yun, Chawon,Kim, Sujeong,Chae, Sunyoung,Lee, Young Ik,Kim, Wook-Hwan,Lee, Jae-Ho,Kim, Wankee,Cho, Hyeseong Japanese Cancer Association 2008 CANCER SCIENCE Vol.99 No.5
<P>Multifunctional activities of the hepatitis B virus X-protein (HBx) in cells have been largely implicated in the development of liver cancer; one of these activities is the loss of p53 function by sequestering p53 in the cytoplasm. We have previously found that doxorubicin increased the p53 levels in cells containing p53-binding HBx protein and restored the p53-mediated transcriptional activity that was suppressed by HBx. Here, we investigated the mechanism underlying p53 reactivation. We found that six phosphorylation sites of the Serine residues of p53 were efficiently phosphorylated in HBx-expressing ChangX-34 cells, suggesting that the binding of HBx to the p53 protein does not interfere with the phosphorylation of p53 by signaling kinases. In addition, doxorubicin caused a dramatic reduction of Hdm2 mRNA and protein levels in cells expressing HBx. Intriguingly, reactivation of p53 was accompanied with a nuclear accumulation of p53 and the phosphorylated p53 at Serine15 was only detected in nuclear fraction, but not in cytosolic fraction of doxorubicin-treated ChangX-34 cells. Functional restoration of the p53 protein in HBx-expressing cells occurs according to the dual effects of doxorubicin: a significant reduction of Hdm2 expression and a nuclear accumulation of the phosphorylated p53 protein. Thus, proper usage of doxorubicin as an effective antitumor agent may be reevaluated and can be extended to tumors primarily caused by infection of DNA tumor viruses.</P>