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Determination of Paraquat in Garlic by LC-MS/MS
ChaeUk Lim,Hyejin Park,unJoo Baek,Jimin Yoon,Juryeong Lee,Byeung Gon Shin,Soon-Kil Cho,Sung-Hee Hong 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11
A fast, sensitive, reliable and economical method was developed for the determination of paraquat (a widely used herbicide) in garlic using iquid chromatography-tandem mass spectrometry (LC-MS/MS). Garlic contains numerous sulfur compounds and strong matrix effect that are difficult to analyze in a single run using HPLC. The sample was extracted with 0.1M HCl under heating. Chromatographic separation of paraquat was achieved on a hydrophilic interaction liquid chromatography column (2.1×50mm, 2.7μm) with a gradient program using 50 mM ammonium formate, 0.5% formic acid and acetonitrile as mobile phase. The low salt concentration used in the eluting buffer ensured extended LC-MS analysis of paraquat in garlic without the necessity of frequent source cleaning. The validity of the developed method was evaluated by spiking paraquat in garlic at 0.01 and 0.1mg/kg. Recovery ranged from 76.5 to 100.0%. The limit of detection is < 0.01mg/kg.
Thematic Poster : TP-40 ; A Case of Isoniazid Induced Lung Fibrosis : Case Report
( Chaeuk Chung ),( Dong Il Park ),( Sun Young Kim ),( Ju Ock Kim ),( Hee Sun Park ),( Jae Young Moon ),( Jeong Eun Lee ),( Choong Sik Lee ),( Sung Soo Jung ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-
A 42-year-old man was diagnosed with tuberculous pleurisy. He was treated with isoniazid (INH), rifampicin (RFP), ethmbutol (EB) and pyrazinamide (PZA). After two weeks of treatment, he developed dyspnea on exercise and symptom got worsened. His arterial O2 saturation was 87% and physical examination revealed velcro rale on both lower lung fields. A chest imaging showed newly developed bilateral lung infiltrations including glass ground opacity (GGO), consolidation, and reticular opacity. Pulmonary function test showed severe restrictive pattern and markedly decreased diffusion lung capacity. Interstitial lung disease induced by anti-Tbc medication was mostly suspected, all drugs were discontinued. Open lung biopsy at right lower lobe revealed chronic interstitial inflammation with fibrosis. Among anti-Tbc medication, INH is most common cause of pneumonitis. At post-operation day 5, anti-Tbc medication except isoniazid was started with prednisolone (Pd) 60mg. After 2 weeks, Pd dose was decreased to 30mg and azathioprine 50mg was added. There are some case reports of INH induced pneumonitis. To our knowledge, this is the first case of INH-induced lung fibrosis occurring in a month of anti-Tbc medication.
( Chaeuk Chung ),( Da Hye Lee ),( Da Hyun Kang ),( Hee Sun Park ),( Dongil Park ),( Jeong Eun Lee ) 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-
Purpose: The efficacy of EGFR-TKIs in lung cancer is limited by various resistance mechanisms. YAP is involved in EGFR-TKI resistance and regulation of PD-L1 transcription. Autophagy is important survival mechanism of cancer cells as a cytoprotective mechanism. The adaptor protein sequestosome 1 (p62/SQSTM1) plays a key role in the autophagic process. p62 also confers genomic instability and functions as signaling hub connecting to mTOR, c-MYC and NRF2. Recently, combination of EGFR-TKI and autophagic blockers such as chloroquine was tried to overcome the resistance of EGFR-TKI, but the result was not satisfactory. Accumulation of oncogenic p62 by chloroquine may have affected the result. So, we tried to find out how inhibit autophagy without p62 accumulation and overcome EGFR-TKI resistance. Method: We investigated the relationship between p62 and YAP by knockdown using specific siRNA or overexpression in the EGFR-TKI resistant lung adenocarcinoma (PC-9/GR cells). Wound-healing assay, transwell migration, and invasion assay were performed to study the effect of targeting YAP and p62. Result: When we knocked down YAP by siRNA in PC9/GR, the protein and mRNA level of p62 were significantly decreased. Consistent with this, YAP overexpression causes the upregulation of p62. We treated YAP siRNA and chloroquine in PC9/GR together, the p62 level remained decreased. We found YAP regulates the expression of p62 via ERK signaling independently of autophagic degradation of p62. Then we used verteporfin which is known as YAP inhibitor to check the effect of blocking autophagy without p62 accumulation. Verteporfin markedly decreased the expressions of YAP, PD-L1 and p62 simultaneously. The combination of EGFR-TKI and verteporfin overcame the EGFR-TKI resistance more effectively than the combination of EGFR-TKI and chloroquine in PC9/GR cells. Conclusion: Our findings suggest Co-targeting YAP/PD-L1 signaling and p62 to overcome EGFR-TKI resistance in lung cancer (Figure 1).
Poster Session : PS 1580 ; DILD : A Case of Isoniazid Induced Lung Fibrosis: Case Report
( Chaeuk Chung ),( Dong Il Park ),( Sun Young Kim ),( Ju Ock Kim ),( Hee Sun Park ),( Jae Young Moon ),( Jeong Eun Lee ),( Choong Sik Lee ),( Sung Soo Jung ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
A 42-year-old man was diagnosed with tuberculous pleurisy. He was treated with isoniazid (INH), rifampicin (RFP), ethmbutol (EB) and pyrazinamide (PZA). After two weeks of treatment, he developed dyspnea on exercise and symptom got worsened. His arterial O2 saturation was 87% and physical examination revealed velcro rale on both lower lung fi elds. A chest imaging showed newly developed bilateral lung infi ltrations including glass ground opacity (GGO), consolidation, and reticular opacity. Pulmonary function test showed severe restrictive pattern and markedly decreased diffusion lung capacity. Interstitial lung disease induced by anti-Tbc medication was mostly suspected, all drugs were discontinued. Open lung biopsy at right lower lobe revealed chronic interstitial infiammation with fibrosis. Among anti-Tbc medication, INH is most common cause of pneumonitis. At post-operation day 5, anti-Tbc medication except isoniazid was started with prednisolone (Pd) 60mg. After 2 weeks, Pd dose was decreased to 30mg and azathioprine 50mg was added. There are some case reports ofiNH induced pneumonitis. To our knowledge, this is the fi rst case of INH-induced lung fi brosis occurring in a month of anti-Tbc medication.