http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Identification of Ligustrum Seedlings Based on Sequence Analysis of an Internal Transcribed Spacer
마상훈,Gianluca Burchi,서정근,Mark S. Roh,정영희 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.5
Two-year old seedlings derived from a Ligustrum quihoui plant (NA56612.01) showed variation in leafsizes, and the leaf sizes of several L. quihoui seedlings were significantly larger than other seedlings, suggesting thatpossibility of interspecific hybridization with L. japonicum or L. foliosum which were maintained sympatrically withthe L. quihoui at the repository. However, the leaves of seedlings were smaller than those of mature L. quihoui plants,thus requiring the use of molecular markers to verify any possible hybridization. Based on the sequence analysis ofthe nuclear internal transcribed spacers 1, 2 in ribosomal RNA gene (ITS 1, 2 region), the sequence of all seedlingsshowed 100% homology and perfectly matched with the L. quihoui accession, but not with L. japonicum or L. foliosum. These data suggested that the seedlings were not resulted from interspecific hybridization, but fromintra-pollination among the L. quihoui progeny. Genetic purity of the L. quihoui can be maintained among seedlingsobtained from the mother plant. This is an important feature for ex situ germplasm conservation of L. quihoui.