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하수기반 역학 바이오 모니터링을 통한 COVID-19 조기검출
김란희 ( Kim Lan Hee ),김상국 ( Kim Sangkook ),윤효직 ( Yoon Hyojik ),김성표 ( Kim Sungpyo ) 한국물환경학회 2020 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2020 No.-
Wastewater-based epidemiology (WBE) monitoring tool has been used to detect illicit drugs and environmental contaminants in wastewater samples collected from the influent of wastewater treatment plants (WWTPs). The WBE can provide spatial and temporal information about the community footprint in the living area. As the coronavirus (COVID-19) disease, which is caused by severe acute respiratory syndrome coronavirus (SARS-CoV) become pandemic in 2019, many countries have employed the WBE monitoring tool enabling early-detection or monitoring of the infected cases (symptomatic and non-symptomatic) in the community without disturbance of individual privacy. However, various COVID-19 detection methods applied in studies made difficulties in method standardization. Besides, the extraction efficiency is depending on the virus properties and the water types. Therefore, evaluation of developed detection methods and data quality analysis should be required. We evaluated and optimized COVID-19 detection methods in the real wastewater influent and sludge samples. To detect COVID-19, four different extraction methods, absorption-elution ultrafiltration, ultracentrifugation, centrifugation/ultrafiltration, and PEG precipitation, have been compared. The viral RNA was extracted using a QIAamp<sup>®</sup> viral RNA mini kit (Qiagen, USA), and the COVID-19 was detected using a GoTaq<sup>®</sup> probe 1-step RT-qPCR (Promega, USA) with two COVID-19 nucleocapsid-specific primers, N1 and N2. The viral community in the samples was analyzed through a NGS-based metagenomic sequencing. This study would help to make a guideline of the COVID-19 detection standard method and to develop quality assurance (QA) and quality control (QC) protocol.
하수기반 역학 바이오 모니터링을 통한 COVID-19 조기검출
김란희 ( Kim Lan Hee ),김상국 ( Kim Sangkook ),윤효직 ( Yoon Hyojik ),김성표 ( Kim Sungpyo ) 한국물환경학회 2020 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2020 No.-
Wastewater-based epidemiology (WBE) monitoring tool has been used to detect illicit drugs and environmental contaminants in wastewater samples collected from the influent of wastewater treatment plants (WWTPs). The WBE can provide spatial and temporal information about the community footprint in the living area. As the coronavirus (COVID-19) disease, which is caused by severe acute respiratory syndrome coronavirus (SARS-CoV) become pandemic in 2019, many countries have employed the WBE monitoring tool enabling early-detection or monitoring of the infected cases (symptomatic and non-symptomatic) in the community without disturbance of individual privacy. However, various COVID-19 detection methods applied in studies made difficulties in method standardization. Besides, the extraction efficiency is depending on the virus properties and the water types. Therefore, evaluation of developed detection methods and data quality analysis should be required. We evaluated and optimized COVID-19 detection methods in the real wastewater influent and sludge samples. To detect COVID-19, four different extraction methods, absorption-elution ultrafiltration, ultracentrifugation, centrifugation/ultrafiltration, and PEG precipitation, have been compared. The viral RNA was extracted using a QIAamp<sup>®</sup> viral RNA mini kit (Qiagen, USA), and the COVID-19 was detected using a GoTaq<sup>®</sup> probe 1-step RT-qPCR (Promega, USA) with two COVID-19 nucleocapsid-specific primers, N1 and N2. The viral community in the samples was analyzed through a NGS-based metagenomic sequencing. This study would help to make a guideline of the COVID-19 detection standard method and to develop quality assurance (QA) and quality control (QC) protocol.