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T subset定量을 위한 抗牛赤血球 IgG抗體의 分離·精製
夏潤文,李振鏞,林壽德 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1981 東西醫學硏究所 論文集 Vol.1 No.1
사람에 있어서 免疫담당세포의 하나인 T 細胞는 몇몇 subpooulation으로 나누어지고 있으며, 그중 ?? 및 ??를 同定하는 수단으로 사용되는 牛赤血球抗體중에서 우선 純粹 IgG抗體를 分離, 精裂하였으며, 이 精裂된 IgG抗體는 標準製品과의 比較實驗에서 ?? 細胞의 一致되는 成積을 얻을 수 있었다. Antisera to ox red blood cell were prepared by intraperitioneal hypermultiple injections without adjuvant in outbred white rabits. Purified IgG fraction from these rabbits anti-ox red blood cell antiserum for T subset assay was obtained by precipitation with 50% saturated ammonium sulphate followed by DEAE-cellulose chromatography and Sephadex G-150 gel filtration. These purified IgG fraction was compared with Cappel company standard IgG fraction for ?? subpopulation assay. We used home-made IgG fraction and obtained favorable results in ?? subpopulation assay as Cappel company standard IgG fraction.
특이혈청항체 IgG 분획(分劃)이 Streptococcus mutans의 평골면(平滑面) 부착(附着)에 미치는 영향에 관한 연구
김찬수,하윤문,이진용,최유진 대한미생물학회 1982 大韓微生物學會誌 Vol.17 No.1
In order to demonstrate the effect of specific serum IgG antibody on the adherence of Streploco- zcus witarrs to smooth surface and the mechanism of effective adherince inbibition by IgG antibody, in the prepent study authors obtained purified 1'gG from different immunogen preparatiohs of S. mufans KCTC I04.49 (serotype c) and observed the effect of each IgG preparation on the adherence of each,S, virstans strain cultured in different conditions. In additiov, the presen( study was under- taken to observe the cross-reactivity of IgG and the eftect of sucrose concentration on the adherence of S. )eutpns in vitro non-growth conditian. The adherence of S. mutans to glass surface was effective]y inhibitecl by serum IgG antibody. At the same IgG concentrations, anti-2Fo fructose grown/1 N NaC1 washedI S. mutans NCTC lp449 cell showed greater a'dherence inJubitory effect to S. mutans strains .than anti 2Zo sccrose grown- .and anti-S. ruutans NCTC 10449 cell wall, and the greater inhibitory effects of Ig6 preparations were observed ia assay using 2z fructose grown S. mutans celI preparations tban using 0. Ip; uarose grown cell preparations. These results suggest that the more effective adherenee inhibition by serum IgG antibody is due to the reaciion with S. mutans cell surface antigens rather $lt;bpn glucan and celI-associated gIucosyltransferase. The greatest adherence inhibitory effeci. Of IgG to S. mulans strains was observed on homologous NCTC 10449 strain ancl the inhibition crogs-reactivities were observed betweein serotype c, e, and f strains. More pronounced cross-reactivity of adherence mhibition oI IgG to S. mutans vas observed in assay using anti-Zi fructose grown/I N XaCl wasbcd cell than using other IgG preparations, and observed in assay usmg 2.$quot;o fructose grosvn S. mutar:s cell preparations thRA'. 0, I a sucrose grown cef.l preparations. It was ir!terested that low, but adequa$lt;e concentration of reactive Igg antibody significantly increased the adherence ability of S, mgtaggg, This result may be due to the formation of srnall cell aggregates resulfed in a iaereage in tJ:e numbers af organisms whieh adhered to glass surface. The adherence of S. mutans to glass surface was possible in the a$sence of gluean-synthotie activity. Low level of sucrose sigdifkaetl increased the. Adherence ability vf S. mutans to glass surface, but excessive amount of suerose indueed Jarge cell aggr,egates reeulted in a decrease in the,aumbers of orgaaism which adbered.