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한국형 B형 간염바이러스 ( Subtype - adr - k ) 의 내면항원 유전자의 염기서열
김용석,현상원,노현모 ( Yong Sok Kim,Sang Won Hyun,Hyune Mo Rho ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.3
The nucleotide sequence of core antigen-coding region of hepatitis B virus (subtype adr-k) cloned in E. coli has been determined by the dideoxy chain termination method using M13 phage template. This sequence we determined is about 1.3 kb long and also includes the open reading frame for gene B (designated gene X previously). Our results showed 96% homology to adr, 92% to adw and 91% to ayw subtype compared with corresponding region of reported sequence. The nucleotide sequence we determined showed differences from reported adr subtype DNA by 27 nucleotides addition at the position of 1791-1817, that was also reported in adw and ayw subtype HBV DNA, and differed by one nulceotide (T) addition at the position 1826. Signal Sequences such as RNA polymerase binding sequences, direct repeat sequences and poly(A) addition site were also detected. Possible roles of these sequences were discussed.
김기태,현상원,김용석,노현모 ( Kee Tae Kim,Sang Won Hyun,Young Sok Kim,Hyune Mo Rho ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4
The complete nucleotide sequence of hepatitis B virus DNA(subtype adr) from a Korean patient has been determined by dideoxy chain termination method. The viral genome is 3213 base pairs long and includes four open reading frames for surface antigen, core antigen, putative DNA polymerase, and B(or X) protein of unknown function in the L strand. It is unique that one nucleotide addition altered B open reading frame. Signal sequences such as direct repeat sequence and poly(A) addition site, and enhancer element were well conserved.
Nucleotide Sequence of the Core Antigen-Coding Region of Hepatitis B Virus (Subtype adr-k)
김용석,현상원,노현모,Kim, Yong-Sok,Hyun, Sang-Won,Rho, Hyune-Mo 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.3
대장균내에 크론잉된 한국형 B형 간염바이러스(adr-k)의 내면항원 유전자(HBcAg)의 염기서열을 M13파아지/dideoxy chain termination 방법을 이용하여 결정하였다. 결정한 염기서열은 약 1.3 kb로서 내면 유전자의 전부분과 후부분을 포함하여 intron이 없는 것으로 생각된다. 보고된 다른 subtype의 염기서열과 비교해 볼 때 adr와는 96%, adw와는 92%, 그리고 ayw와는 91%의 염기서열이 같음을 알 수 있었다. 이미 보고된 adr에 없던 27개의 염기서열이 adr-k에는 1791-1817위치에 나타났는데, 이는 adw나 ayw에서는 나타났었다. 또한 1826 위치에 하나의 염기(T)가 더 끼어 있음이 특징이다. HBcAg 유전자의 앞부분에 조절 및 기능부위로 보이는 RNA polymerase binding sequence, direct repeat sequences 및 poly(A) addition site가 발견되었으며, 이들의 signal siquences에 대한 가능한 역할에 대해서 논의하였다. The nucleotide sequence of core antigen-coding region of hepatitis B virus (subtype adr-k) cloned in E. coli has been determined by the dideoxy chain termination method using M13 phage template. This sequence we determined is about 1.3 kb long and also includes the open reading frame for gene B (designated gene X previously). Our results showed 96% homology to adr, 92% to adw and 91 % to ayw subtype compared with corresponding region of reported sequence. The nucleotide sequence we determined showed differences from reported adr subtype DNA by 27 nucleotides addition at the position of 1791-1817, that was also reported in adw and ayw subtype HBV DNA, and differed by one nulceotide (T) addition at the position 1826. Signal Sequences such as RNA polymerase binding sequences, direct repeat sequences and poly(A) addition site were also detected. Possible roles of these sequences were discussed.
Complete Nucleotide Sequence of Hepatitis B Virus (subtype adr)
김기태,현상원,김용석,노현모,Kim, Kee-Tae,Hyun, Sang-Won,Kim, Yong-Sok,Rho, Hyune-Mo 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.4
대장균내에 클로닝된 한국형 B형 간염 바이러스(subtype adr)의 전체염기서열을 M13 파아지/dideoxy chain termination 방법으로 결정하였다. 이 바이러스의 전체 genome은 3213 bp였으며 4개의 open reading frames(S, C, P and B)를 가지고 있었다. 우리의 전체염기서열을 보고된 다른 subtype과 비교할 때 adr과는 97%, adw와는 91%, ayw와는 90%의 염기서열이 동일한 것을 알 수 있었다. 여러 subtype간에 염기서열과 아미노산서열을 비교 연구하였다. 특히 하나의 염기(T)가 더 끼어 있어 B 유전자의 reading frame이 변경되었다. Direct repeat sequence와 poly(A) addition site 그리고 enhancer element가 확인되었다. The complete nucleotide sequence of hepatitis B virus DNA(subtype adr) from a Korean patient has been determined by dideoxy chain termination method. The viral genome is 3213 base pairs long and includes four open reading frames for surface antigen, core antigen, putative DNA polymerase, and B(or X) protein of unknown function in the L strand. It is unique that one nucleotide addition altered B open reading frame. Signal sequences such as direct repeat sequence and poly(A) addition site, and enhancer element were well conserved.