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인간의 암세포에서 새로운 내생레트로바이러스 HERV - S LTR 의 계통분류
이주미(Joo Mi Yi),김희수(Heui Soo Kim) 한국유전학회 2001 Genes & Genomics Vol.23 No.4
A new human endogenous retroviral family(HERV-S) was recently identified from a human X chromosome that was 6.7 kb in length and had a typical retroviral structure with LTR-gag-pol-env-LTR. Using the PCR and sequencing approach, we investigated LTR elements of the HERV-S family from genomic DNA of the human cancer cells(MCF7, SiHa, HepG2, 2F7, AZ521). Seven LTR elements were newly identified from the human cancer cells and had a high degree of sequence similarity with that of the HERV-S. Phylogenetic analysis from the HERV-S family showed that the LTR elements were mainly divided into 2 groups through evolutionary divergence in human evolution, suggesting that multi-copy of the HERV-S LTR elements could exist the human genome with at least two different types of HERV-S. These HERV-S LTR elements deserve further investigation as potential leads in the study of human cancers.
Molecular Cloning of HERV-W LTR Family from Human Brain
Kim, Heui-Soo,Yi, Joo-Mi,Kim, Tae-Hyeong,Lee, Ji-Won,Kim, Myung-Sook 한국유전학회 2002 Genes & Genomics Vol.24 No.2
Long terminal repeat (LTR) elements of the human endogenous retrovirus (HERV) have been found to be coexpressed with sequences of genes closely located. It has been suggested that the LTR elements have contributed to the structural change or genetic variation of human genome connected to various diseases and evolution. The HERV-W family has been identified in the cerebrospinal fluids and brains of individuals with schizophrenia. Using cDNA library derived from human brain tissue, we performed PCR amplification and identified five new HERV-W LTR elements. Those LTR elements showed a high degree of sequence similarity (96∼99%) with the HERV-W LTR (AF072500). A phylogenetic tree obtained by the neighbor joining method revealed that new HERV-W LTR elements (HB-1 and HB-3) were closely related to the HERV-W LTR family, AX000960, AF072504, and AF072506, from GenBank database including the HERV-W LTR. Compared to the HERV-W LTR elements, clones HB-11, HB-12, and HR-15 had additional sequences (CTGTTTGCCA CCACCA) specifically. The data indicate that several copy numbers of the HERV-W LTR elements are transcribed in human brain and may contribute to an understanding of brain function in relation to neuropsychiatric diseases.
Molecular Phylogeny of Endogenous Retrovirus HERV-F Family in Japanese and Rhesus Monkeys
Kim, Heui-Soo,Jeon, Seung Heui,Yi, Joo-Mi,Kim, Tae-Hyeong,Kim, Myung-Sook,Hyun, Byung-Hwa,Takenaka, Osamu 한국유전학회 2002 Genes & Genomics Vol.24 No.2
A new human endogenous retroviral family (HERV-F) has recently been identified from the human chromosome 7q31.1-q31.3 that was identical to the XA34 cDNA clone isolated from a human glioma cDNA library with an ERV-9 probe. The current study investigated pol fragments of the HERV-F family from Japanese and rhesus monkeys and compared them with those of the HERV-F (Hu-XA34) family. Fourteen pol fragments of the HERV-F family were detected from the monkeys, which showed a 78.7-95.4% sequence similarity with those of HERV-F (Hu-XA34). Clones FJM-1, FJM-7, FJM-14, and FJM-15 from the Japanese monkey and FRH-1 and FRH-4 from the rhesus monkey exhibited no disruption due to point mutation or insertions/deletions. The ratio of synonymous to non-synonymous substitutions indicated that negative selective pressure was acting on these clones. Therefore, the pol gene sequences could be associated with an active provirus in the monkey genomes. A phylogenetic analysis of the pol fragments from humans and monkeys using the neighbor joining and maximum parsimony methods showed six groups, indicating that either the HERV-F family was amplified at least six times after its original integration into the monkey genome or the occurrence of independent integration events during primate evolution.
인간의 뇌조직에서 만들어진 cDNA library 에서 내생레트로 바이러스 HERV - K 로부터 유래된 레트로포존의 계통분류
이지원,김희수,이주미,신경미 한국유전학회 2001 Genes & Genomics Vol.23 No.3
SINE-R retroposons, derived from the human endogenous retrovirus HERV-K family, have been found to be hominoid-specific. Both SINE-R retroposons and HERV-K family have potential relevance to recent genome change and various human diseases. In this report, we identified seven SINE-R retroposons from the human brain cDNA library and compared them with sequences that we have previously reported in humans and hominoid primates. The SINE-R retroposons derived from human brain cDNA library showed 91∼95% sequence similarities with human-specific retroposon SINE-R.C2. They also showed 88∼92 % sequence similarities with that of the Schizo-cDNA clone that derived from postmortem tissue from the frontal cortex of & individual suffering from schizophrenia who committed suicide at the age of thirty-four. Phylogenetic analysis using the neighbor-joining method revealed that three SINE-R retroposons (HB-4, HB-5, HB-7) from the human brain cDNA library were closely related to the human-specific retroposon SINE-R.C2. The data indicate that such retroposons transcribed in the human brain represent a source of genetic variation related to neuropsychiatric diseases.