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정상 또는 고지방식을 섭취한 흰쥐에서 Casein 펩타이드 분획물이 혈청 지질농도에 미치는 영향
오주환(Ju-Hwan O),이연숙(Yeon-Sook Lee) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.2
본 연구에서는 casein 펩타이드 분획물이 정상 및 고지방식을 섭취한 흰쥐에서의 혈청 및 조직의 지질 농도에 미치는 섭취효과를 검토하고자 하였다. 정상 지방식이(7% soybean oil & cholesterol-free; Expt I)를 섭취한 흰쥐는 정상 혈청 지질농도를 나타내는 것으로, 분배설량이 약간 증가하는 경향을 나타내긴 하였지만 이때 casein 펩타이드 분획물군의 혈청, 간조직의 지질농도에 대한 효과는 유의적인 차이가 없었다. 하지만, 순환기계질환의 주요 인자인 HDL/LDL비는 친ㆍ소수성 펩타이드에서 유의적으로 높은 경향을 나타내었다. 고지방ㆍ콜레스테롤식이(18% beef tallow & 1% cholesterol; Expt Ⅱ)를 급여하였을 경우, 친ㆍ소수성펩타이드 분획물군에서 분중으로의 총지질, 총콜레스테롤 및 중성지방의 배설량이 유의적(p<0.05)으로 또는 증가하는 경향을 나타내었다. 이런 결과, 고지방식이를 급여했을 때 친ㆍ소수성펩타이드 분획물군이 casein군에 비해 혈중 및 간조직 중의 지질농도가 낮아지는 경향을 나타냈다. 또한 HDL/LDL비도 casein군에 비해 친ㆍ소수성 펩타이드 분획물군에서 높게 관찰되는 것으로, 이는 고지혈증 위험요소를 저하시키는 효과가 있는 것으로 사료되었다. 친ㆍ소수성 펩타이드 분획물(CL & CB)의 아미노산 조성 결과, 친ㆍ소수성 펩타이드 식이의 glycine과 methionine함량은 casein 식이의 조성과 거의 비슷한 데, arginine과 lysine함량은 casein 식이의 조성과 상당히 달랐다. 또한 혈중 지질농도를 낮추는 것으로 보고되어지는 arginine/lysine 비와 glycine/methionine 비는 친ㆍ소수성 펩타이드분획물 식이(CL & CB)에서 낮게 관찰되었다. 이러한 결과는 동물실험 결과와 같이 고찰해볼 때, 아미노산 조성이 혈중 및 조직 중의 지질저하 효과에 미치는 영향이 그다지 크지 않은 것으로 사료되었고, 앞으로 이에 대한 연구가 더욱 필요하였다. 친ㆍ소수성 casein 펩타이드 분획물의 지질 대사에 미치는 영향을 관찰하여 보았을 때, 고지혈증 및 고콜레스테롤혈증 흰쥐에서 혈중 및 간조직의 지질함량을 저하시키는 효과가 있는 것으로 나타났다. 이에 가능한 기전으로는 분중으로의 지질 배설량을 증가시킨 것에 기인한 것으로 해석되었으며, 섭취기간이 길어질수록 효과가 확실해질 것으로 기대되었다. 또한 casein 펩타이드 분획물의 아미노산 조성비의 차이라기보다는 펩타이드 자체의 효과임이 시사되었다. In the recent studies, many researchers are interested in foods as functional components rather than nutrient sources. Cow's milk is considered as an excellent food sources because of its many nutrients. Casein is a major milk protein and has been reported to have hyperlipidemic and hypercholesterolemic effects. But several reporters have suggested that peptide fractions and hydrolysate of casein have hypolipidemic effects differing from intact protein, casein. Therefore, the objective of the study was to investigate how the casein peptide fractions affect lipid metabolism in rats fed normal or high fat diets. The peptide fractions and hydrolysate of casein were obtained by casein hydrolysis with trypsin. The male rats (Sprague-Dawley), weighing approximately 150 g, were fed each experimental diet containing casein (CAS), casein hydrolysate (CH), casein hydrolysate precipitate (Cpt) and two kinds of peptide fractions (CL & CB) for three weeks, respectively. In the expt I, the male rats were fed normal fat diets (7% soybean oil & cholesterol-free; Expt. I), and in the expt II, fed high fat diets (18% beef tallow & 1% cholesterol; Expt. II). Crude protein contents were calculated from nitrogen contents. Amino acid composition of each fraction was also analyzed. The concentration of total lipid, total cholesterol and triglyceride in serum, liver and feces were measured. As the results of study, the rats fed peptide fractions with normal fat diets (Expt. I) had no effects on total lipid, total cholesterol and triglyceride concentration in serum and liver and fecal excretion. However, in the rats fed hydrophobic casein peptide fractions (CB) with high fat diet, fecal lipids excretion were significantly increased and the lipids concentration of serum and those of liver tended to decrease, numerically. Also in the rats fed hydrophilic casein peptide fractions (CL) with high fat diet, total lipids and triglyceride concentration of serum were significantly decreased. And the lipids concentration of liver tended to decrease and fecal lipids excretion tended to increase, numerically. These results suggested that the possible hypolipidemic and hypocholesterolemic effects of both casein peptide fractions (CL & CB) could be partly due to increased fecal lipids excretion. And this effects might depend on dietary fat contents, which is high fat diet (18% beef tallow & 1% cholesterol diets).
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미용전공 여대생과 비전공 여대생의 체형인식, 신체만족도 및 식습관 비교 연구
이종현,김민선,오주환,Lee, Jong-Hyun,Kim, Min-Sun,O, Ju-Hwan 동아시아식생활학회 2007 동아시아식생활학회지 Vol.17 No.4
This study was carried out to investigate perceptions of body image, body satisfaction, and dietary habits of beauty art major and non-major college students in Gyeonggi province. A total of 312 self-administered questionnaires (beauty art majors=145; non-majors=167) were analyzed. The means for height, weight, and BMI were 161.7 cm, 51.4 kg and 19.7, respectively. There were more majors who were underweight according to BMI classification than non-majors. Sixty-six percent of the subjects had previous weight control experience, and there was no significant difference between the majors and non-majors. The mean score for current body image was 4.61 out of a possible 9 points in the majors, which was significantly lower than 4.95 in the non-majors, and their perception of an ideal body image was thinner than their current body image. Those with more weight control experience had currently heavier perceptions of their body. The mean score for body satisfaction was 2.60 out of a possible 5 points, which was lower than the mean score for their attitudes toward the importance of their bodies. The mean score for dietary habits was 2.80 out of a possible 5 points, and there was no significant difference with dietary habits according to weight control experience or BMI classification. In both the majors and non-majors, there was a significant positive correlation between BMI and perception of current body image (p<0.001, p<0.001), and a negative correlation between BMI and body satisfaction (p<0.01, p<0.001). In the major students, there were significant positive correlations between dietary habits and body satisfaction (p<0.01), and attitudes toward the body importance (p<0.05); therefore, the greater their body satisfaction and body importance, the higher their scores for dietary habits.
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이종현,오주환 동남보건대학 2003 論文集-東南保健大學 Vol.21 No.2
This study was carried out to investigate dietary habits and related factors of female high school and college students in Gyeonggi Province. The total of 202 self-administered questionnairs(high school students, 77; college students, 125) were analyzed. Mean height and weight were 161Acm and 53.5kg, mean BMI was 20.6. The percentage of high school students who had adequate sleep was 46.2% and that of college students was 22.0%. Only 2.6% of high school students and 27.2% of college students answered they exercised regularly(p<0.001). The percentage of skipping breakfast and overeating was 70.3% and 88.6%, respectively. Frequency of outside eating was higher in college students than high school students(p<0.001). The percentage of those who used to intake over 8 cups of water and beverage daily was 10.4%. Average scores of food intake frequency showed 1.87 out of a possible 3 points, scores of vegetable intake frequency were significantly higher in the college students than high school students(p<0.05). The more sleep and the better physical health status they had, the more regularly they had breakfast and eating time. Unbalanced diet, and outside eating negatively correlated with regular exercise, and sleeping hours, respectively. Regular breakfast showed positive correlation with frequency and time of eating. The more regularly they had breakfast and eating time, the lower frequency of overeating and outside eating was. Scores of food intake frequency were significantly correlated with frequency of eating, regular breakfast, eating time, speed of eating and overeating.
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