한국의 임상과 자연환경에서 분리된 Cryptococcus neoformans의 혈청형과 효소생성능

황수명,오광석,이경원,Hwang, Soo-Myung,Oh, Kwang-Seok,Lee, Kyung-Won 한국미생물학회 2006 미생물학회지 Vol.42 No.4

Fifty eight Cryptococcus neoformans strains isolated from clinical and environmental sources in Korea were examined for their serotypes and extracellular enzyme activities. Among the 51 strains isolated from clinical sources, 48 strains were serotype A (94.1%), 2 strains were serotype B (3.92%), and 1 strain was serotype D (1.96%). All seven environmental strains isolated from pigeon excreta were identified as serotype A. All isolates of C. neoformtans were positive for the production of extracellular proteinase and phospholipase. In the API-ZYM system, all fifty eight isolates produced alkaine phosphatase, esterase C4, esterase lipase: C8, leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrase, $\alpha$-glucosidase and $\beta$-glucosidase. Thirty nine isolates (67.2%) of C. neoformans produced N-acetyl-$\beta$-glucosidase. Two isolates, serotype B, and B only one serotype A produced $\beta$-glucuronidase. Analysis of enzymatic profiles to 21 enzymes revealed four biotypic patterns among the 58 strains. The enzymatic patterns of C. neoformans isolated from clinical and environmental sources represented a significant relationship with the serotypes. 한국의 임상검체와 자연환경에서 분리된 Cryptococcus neoformans 58주에 대한 혈청형과 세포외효소 생성능에 관한 셍물학적 특성을 조사하였다. 환자로부터 분리된 임상균주 51주 중 48주는 혈청형 A (94.1%) 였으며 2주는 혈청형 B (3.92%),그리고 나머지 1주는 혈청형 D (1.96%)였다. 자연환경에서 분리된 7주는 비둘기 분변에서 분리된 것들이며 모두 혈정형 A였다. 모든 균주는 proteinase와 phnospholipase를 생성하였고, 또한 API-ZYM system을 이용한 19종류의 효소생성능 시험에서는 alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, $\alpha$-glucosidase, 그리고 $\beta$-glucosidase를 생성하였으나, N-acetyl-$\beta$-glucosarninidase는 39주 (67.2%)에서만 생성하였다. 혈청형 B로 동정된 2주와 혈청형 A로 동정된 균주중 1주는$\beta$-glucuronidase를 생성하였다. 본 연구에 사용된 총21종류의 효소 생성능 시험을 기초로 하여 생물형을 구분하였는데, 모두 4가지의 유형을 나타내었고, 또한 임상과 환경균주에서 혈정형과 생물형 특성간의 유의한 상관성를 나타내었다.

Bacillus firmus NA-1 균주와 Bacillus subtilis GT-D 균주를 이용한 발효비지의 기능성

오수명(Soo-Myung Oh),김찬식(Chan-Shick Kim),이삼빈(Sam-Pin Lee) 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.1

일본 청국장에서 분리된 Bacillus firmus NA-1과 재래청국장에서 분리한 Bacillus subtilis GT-D를 이용하여 전지활성 생 대두미세분말(MFS) 첨가 및 발효시간에 따른 비지 발효물의 혈전용해효소, 가수분해효소, 점질물, 펩타이드 생산 및 풍미개선 효과를 알아보았다. 84%의 수분함량을 포함하는 비지에 대두미세분말을 10, 15, 20% 첨가함으로써 78, 74, 70%로 수분함량을 감소시킬 수 있으며, 발효시간이 경과할수록 tyrosine함량이 증가하였으며, B. firmus NA-1 균주보다는 B. subtilis GT-D 균주를 사용하는 경우에 높은 값을 보였다. 발효비지의 점조도는 B. firmus NA-1 균주를 이용한 발효물이 높은 값을 보였으며, 두 균주 모두 MFS 20%를 첨가한 후 26시간 발효한 발효물에서 2.18, 0.35 ㎩ㆍsⁿ로 가장 높은 점조도 값을 나타내었다. 혈전용해효소 역시 B. firmus NA-1 균주를 이용한 비지청국장에서 10%이상의 높은 활성을 보였으며, 발효 22시간까지 증가하다가 26시간부터는 큰 변화가 없거나 감소하는 경향을 보였다. 따라서 20%의 MFS를 첨가한 후 42℃에서 22시간 동안 발효하는 것이 발효취 생성을 최소화하는 조건이라 사료되었다. 비지청국장의 동결건조는 청국장 냄새 및 수분함량을 6%수준으로 줄일 수 있었으며, 혈전용해효소의 활성을 포함하였다. B. firmus NA-1 균주를 이용한 시료에서는 615 unit/g의 가장 높은 protease의 활성을 보였으며, B. subtilis GT-D 균주를 이용한 시료에서는 1903 ㎎%의 tyrosine 함량 및 180 unit/g의 α-amylase의 활성을 나타내었다. To convert the soybean curd residue (SCR) to functional food ingredient, alkaline fermentation of SCR was performed by Bacillus firmus NA-1 and Bacillus subtilis GT-D for 22 hr at 42℃. The micronized full-fat soy flour (MFS) was fortified to reduce the moisture content as well as to supply protein source. The mucilage and flavor productions in the fermented SCR were enhanced by the fortification of 20% MFS. The peptide production from the SCR fermented with B. subtilis GT-D substantially increased when judged by the detectable amount of tyrosine (480 ㎎%). The production of fibrinolytic enzyme was increased by the fermentation for 22 hr, indicating the relative activity of 62% (B. firmus NA-1) and 47% (B. subtilis GT-D), respectively. The SCR fermented by B. firmus NA-1 and B. subtilis GT-D indicated the consistency of 1.95 ㎩ㆍsⁿ and 0.21 ㎩ㆍsⁿ, respectively. After freeze-drying, the protease activity (615 unit/g) and α-amylase activity (180 unit/g) were obtained from SCR fermented by Bacillus firmus NA-1 and Bacillus subtilis GT-D, respectively.

Bacillus firmus NA - 1 균주를 이용한 비지로부터 혈전분해능효소 및 펩타이드 생산

오수명(Soo-Myung Oh),서지현(Ji-Hyun Seo),이삼빈(Sam-Pin Lee) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.6

일본 청국장에서 분리된 Bacillus firmus NA-1를 이용하여 비지의 수분함량, 생대두미세분말첨가 및 발효시간에 따른 비지 발효물의 혈전용해효소, 점질물, 펩타이드생산 및 풍미개선 효과를 알아보았다. 비살균 D-비지는 30시간 이상부터 청국장 발효가 진행되는 반면, 살균 D-비지는 18시간부터 고초균 발효에 의하여 pH가 증가하였으며 혈전분해효소, 펩타이드 함량이 비살균 D-비지보다 높은 값을 보였다. P-비지의 경우는 18시간 발효시에 가장 높은 pH, tyrisone 함량과 혈전용해효소활성을 나타냈다. 또한 P-비지를 건조하여 초기수분함량을 60%로 낮추었을 때 발효비지물의 펩타이드 함량과 혈전용해분해효소활성이 증가하였다. P-비지에 전지활성 생대두미세분말을 10%수준으로 첨가함으로써 혈전분해효소 및 펩타이드 함량이 증가되는 효과를 보였으며, 점질물의 생성에 따른 점조도 값의 증가를 나타내었다. 특히, 콩미세분말을 20% 수준으로 첨가하여 발효시킨 비지는 고유한 청국장의 풍미를 나타내었으며, 건조시킨 발효비지물의 조단백질함량은 27.3%를 나타내었다. 따라서 비지의 고초균 발효시에 전지활성 생대두미세분말의 첨가는 비지의 초기수분함량을 낮추면서, 고초균 발효의 촉진을 통한 콩 펩타이드 및 혈전용해효소의 증가, 점질물 생산, 풍미 개선을 비롯하여 발효비지의 단백질 보강이 가능하였다. To produce functional food ingredient from the soybean curd residue (SCR), alkaline fermentation was performed with SCR from cold processed (D-SCR) or hot processed (P-SCR) tofu. The solid-state fermentation was performed by Bacillus firmus NA-1 at 42℃. The fermentation of heat-treated D-SCR resulted in higher production of peptides and fibrinolytic enzyme compared with D-SCR without heating. The P-SCR showed higher production of peptides, fibrinolytic enzyme, indicating alkaline pH after fermentation for 18 hr. When the moisture content of P-SCR was reduced to 60%, the production of peptides and fibrinolytic enzyme were enhanced. The P-SCR fortified with 10% MFS (micronized full-fat soy flour) showed higher fibrinolytic enzyme activity and consistency index by fermentation of Bacillus firmus NA-1. Furthermore, the P-SCR fortified with 20% MFS indicated relatively higher peptide content, fibrinolytic enzyme activity and enhanced flavor. By increasing the addition of MFS, the peptide content of fermented P-SCR was increased significantly, but fibrinolytic enzyme was slightly decreased.

철근 콘크리트 슬래브의 디자인이 동적 거동에 미치는 영향

오경윤 ( Oh Kyung Yoon ),조진구 ( Cho Jin Goo ),최수명 ( Choi Soo Myung ),홍종현 ( Hong Chong Hyun ) 한국농공학회 2007 한국농공학회논문집 Vol.49 No.6

This paper is on the research of the special character of the dynamic response according to a design of the clamped reinforced concrete slab. In this study, the 20-node solid element has been used to analyze the dynamic characteristics of RC slabs with clamped edges. The elasto-visco plastic model for material non-linearity and the smeared crack model have been adopted in the finite element formulation. The design factor, which affect the dynamic response of the reinforced concrete slab, are the steel layer thickness, steel layer depth, steel layout method, steel layout angle and the slab thickness and span ratio. The main purpose of this study was to find out the dynamic response of the reinforced concrete slab according to above variables. The reduction of deflection/thickness ratio appeared less than 2% when the slab thickness between 20 and 21cm. It is desirable that the slab thickness must be above 20-21cm. The reduction ratio of deflection is appeared greatly when the value of the span/thickness ratio is between 25 and 30. In conclusion, the steel layer depth and thickness had a little effect on deflection of the dynamic response, but had no effect on the steel layout angle.

소아에서 발생한 췌장의 유두상 낭성 종양 2예

최성일,오수명,Choi, Sung-Il,Oh, Soo-Myung 대한소아외과학회 2000 소아외과 Vol.6 No.2

Solid and papillary cystic neoplasm of pancreas is an uncommon low grade malignant tumor. It is predominant in young female between the second and third decades of life, and amenable to cure by surgical treatment. The authors report two cases of solid and papillary neoplasm of pancreas pathologically verified at Kyung Hee University Hospital. The first case was an 11-years old female patient and the other case was a 12-years old male. Symptoms were abdominal discomfort, nausea and vomiting in both cases and abdominal pain in the female patient. CT finding included a solid and papillary neoplasm of pancreas. The mass was well-demarcated with solid and cystic necrosis components. In the female patient, a large hematoma was found. Gross findings revealed apparent encapsulation, cystic degeneration and hemorrhagic necrosis. Microscopically the tumors were characterized by distinctive solid and papillary patterns of cellular arrangement without local invasion. Both patients were discharged after surgery and followed up without any problem.

후장중복증을 동반한 변형 총배설강외반증

이태석,오수명,Lee, Tae-Seok,Oh, Soo-Myung 대한소아외과학회 1995 소아외과 Vol.1 No.2

Diphallus is a rare congenital anomaly and is frequently associated with duplication of the urinary tract and rectosigmoid, and commonly associated with vertebral anomalies. Remzi reported less than 100 cases of duplication of all or a portion of the penis, but about 10 cases of complete diphallus with exstrophy of cloaca was reported, and a case of complete diphallus associated with hindgut duplication was reported, and complete diphallus with displacement of bladder associated hindgut duplication and imperforate anus was not reported in Korea. We experienced a case of the complete diphallus associated with displacement of bladder, hindgut duplication, and imperforate anus as a variant of cloacal exstrophy. A review of published cases suggests that this may be the first example of a complete diphallus with displacement of bladder coexisting with the hindgut duplication and imperforate anus.

분포하중이 철근 콘크리트 슬래브의 동적 거동에 미치는 영향

오경윤 ( Oh Kyung-yoon ),조진구 ( Cho Jin-goo ),최수명 ( Choi Soo-myung ),홍종현 ( Hong Chong-hyun ) 한국농공학회 2008 한국농공학회논문집 Vol.50 No.2

This study has been carried out to investigate the dynamic characteristics of RC slabs. For this purpose, the 20-node solid element has been used to discretize the RC slabs into two parts of concrete and rebar. The material non-linearity considering elasto-visco plastic model and the smeared crack model have been adopted in the finite element formulation. The applied load can handle step load, load intensity of harmonic load, area of distributed load and frequency. The frequency of harmonic load has an significant effect on dynamic behaviour in terms of displacement. As the frequency is increased, the effect of load amplitude is more serious. Especially, if the frequency of harmonic load exceeds 30 Hz, it is noted that the displacement by harmonic load is greater than that by step load. In case of harmonic load, the damping effect shows no certain tendency with respect to frequency of load. In details, the damping is effective when the frequency of harmonic load is 2 Hz, but there is no consistent tendency according to damping ratio. The dynamic response when the frequency of harmonic load is 3 Hz shows same result for undamped case as well as for damped case with 5% damping ratio. It is also noted that we can get the largest deflection for damped case with 1% damping ratio. However, there is not any damping effect when the frequency of harmonic load is greater than 4 Hz.

농업경영체 등록정보와 공간데이터를 활용한 밭 이용 방안 연구: 전라남도 무안군·해남군을 중심으로

오윤경 ( Oh Yun-gyeong ),유승환 ( Yoo Seung-hwan ),최수명 ( Choi Soo-myung ),이지민 ( Lee Jimin ) 한국농공학회 2018 한국농공학회논문집 Vol.60 No.4

Food consumption pattern changes, including a decrease in rice consumption and increasing demand for fresh vegetables, along with declining food self-sufficiency rate, more importance is being placed on agricultural industry within Korea. Taking these changes into consideration, there is an increasing necessity for managing upland fields on agricultural sector to revitalize agricultural land use. Therefore, in this study, upland use type was classified into agricultural economic, environmental conservation, and rural social frames. The purpose of this study was to suggest upland use plan with farm manager registration information and spatial data. As results of this analysis, the mean area of agricultural economic frame in Haenam (16.47 ha) was found to be wider than Muan (3.17 ha), and scale improvement zones (54 zones) were located in only Haenam. In case of environmental conservation frame, there’s no zone of scenic agriculture land in both study areas, but landuse transition zones were located in Muan (278 zones) and Haenam (604 zones). Agro-healing zones of rural social frame were 1,018 zones in Muan and 1,588 zones in Haenam and kitchen garden sites were 342 zones in Muan and 370 zones in Haenam. These results could be used when we establish the plan of an agricultural infrastructure project or select places for a collaborative agricultural land use project.

장충첩증을 일으킨 소장의 선근종 1예

이희수(Hee Soo Lee),이태석(Tae Seok Lee),오수명(Soo Myung Oh) 대한외과학회 1992 Annals of Surgical Treatment and Research Vol.42 No.5

배양된 상피세포를 이용한 광범위 화상의 치료와 피부재건에 관한 연구

박호철(Ho Chul Park),오수명(Soo Myung Oh),주홍재(Hoong Zae Joo),박재경(Jae Kyung Park),최용묵(Yong Mook Choi) 대한외과학회 1989 Annals of Surgical Treatment and Research(ASRT) Vol.37 No.2