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      저자 : 신유옥 ( Yuock Shin ) (검색결과 2 건)
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      • KCI등재

        LPS로 유발한 장염증 모델에 대한 주초황금의 항산화 조절 및 장염증 개선 효과

        신성호 ( Sungho Shin ),신유옥 ( Yuock Shin ),이주영 ( Jooyoung Lee ),이아름 ( Ahreum Lee ),김민영 ( Minyeong Kim ),박찬흠 ( Chanhum Park ),서부일 ( Buil Seo ),노성수 ( Seong-soo Roh ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.4

        Objectives: The aim of present study was to evaluate the beneficial effect of Scutellariae Radix (SR) and Scutellariae Radix EtOH-heated at 200℃ (SR200) using lipopolysaccharide (LPS) treated intestine of mice. Methods: Extract of SR and SR200 were orally administrated. Their effects were compared with vehicletreated LPS and normal groups. Subsequently, we measured reactive oxygen species (ROS) and nitric oxide in the serum and western blotting in the intestine. Results: The average weight in LPS treated (Vehicle) group was lowered significantly compare to that in non-treated normal group and this weight loss in the vehicle group was effectively prevented by the administration of SR and SR200 respectively. The increased oxidative stress biomarker levels such as reactive oxygen species (ROS) and nitric oxide (NO) in the serum was markedly decreased by treated with SR200. The decreased levels of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) induced by LPS injection were significantly restored by both SR and SR200 treatment. Moreover, increased inflammatory mediators and cytokines such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF- ) and interleukin-6 (IL-6) in the LPS treated vehicle mice were significantly decreased through down-regulation c-JUN through reduction of oxidative stress. Conclusions: SR and SR200 could have benefit effect through down-regulation of abnormal oxidative stress in LPS induced intestine injury mice. Moreover, The anti-inflammatory activity of SR200 extract was better than SR extract in the LPS induced intestine injury mice.

      • KCI등재

        대황 감초 복합추출물의 항산화 효과를 통한 역류성 식도염 개선 효과

        김민영 ( Minyeong Kim ),신유옥 ( Yuock Shin ),이주영 ( Jooyoung Lee ),이아름 ( Ahreum Lee ),신성호 ( Sungho Shin ),권오준 ( Ojun Kwon ),서부일 ( Buil Seo ),노성수 ( Seong-soo Roh ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.4

        Objectives : The present study was designed to evaluate the anti-inflammatory and anti-oxidative stress activities through regulation of Nrf2-mediated genes by Rhei rhizoma and Glycyrrhiza rhizoma combined extract (RGE) in reflux esophagitis. Methods : The antioxidant activity of RGE in vitro was measured in terms of radical scavenging capacity such as DPPH and ABTS. RGE was administered at 350 mg/kg body weight prior to induction of reflux esophagitis. Reflux esophagitis was induced that tied the pylorus and the transitional junction between the forestomach and the corpus in Sprague-Dawley rats. Results : RGE scavenged DPPH and ABTS effectively and IC50 of RGE each were 4.9 μg/ml and 45.6 μg/ml. Our results show that RGE administration markedly ameliorated mucosal damage upon histological evaluation. In serum and esophagus tissue, RGE significantly suppressed the oxidative stress biomarkers. Reflux esophagitis induced rats exhibited down-regulation of antioxidant-related proteins in the esophagus; however, the levels with treatment of RGE were significantly higher than those of vehicle reflux esophagitis rats. RGE treatment caused significant reductions in activation of NF-κB transcription factor. Thus, RGE significantly exhibited potent anti-inflammatory activities by suppressing the protein expression levels of pro-inflammatory proteins such as COX-2 and iNOS and inflammatory cytokines such as TNF-α in the esophagus tissue. Conclusions : Reflux esophagitis caused considerable levels of oxidative stress in the esophageal mucosa and the administration of RGE reduced the esophageal mucosa damage through the regulation of Nrf2 and NF-κB pathways. Our findings can considered as supplementary therapy in the prevention or treatment of reflux esophagitis.

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