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Methotrexte 투여가 생쥐 장관의 Alkaline Phosphatase 활성에 미치는 영향
이여일(YI Lee),박원집(WJ Park),문병갑(BK Moon) 대한산부인과학회 1980 Obstetrics & Gynecology Science Vol.23 No.12
This experiment was performed to study the effects of methotrexate on the mucus secretion and alkaline phosphatase activity of mucosal epithelium in gut. Methotrexate was administered intraperitoneally in a dose of 0.5mg/mouse 48 hours interval. Experimental groups were divided into the single, two times and three times treated ones. Mouse were sacritied 24 hours after final treatment and stains for H&E, PAS and alkaline phosphatase were made in section of jejunum, ileum and colon. The result obtained were as follows. 1. Denudation of surface mucosal epithelium and abnormal dilatation of intestinal glands were prominent in small intestine as compared with those in large intestine, following methotrexate treatments. 2. Reduction of PAS positive materials was shown in the goblet cells and intestinal glands in a proportion to methotrexate treatments, which was more prominent than that of large intestine. 3. Activity of alkaline phosphatase was increased in lamina propria, intestinal glands, and submucosal tissue of jejunum and ileum but decreased in surface mucosal epithelium and exfoliated epithelium in a proportion to methotrexate treatments, which are milder in colonic mucosa than that of small intestine.
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