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브로일러 병아리의 등 피부에 312 mm 자외선 조사시 상이한 선량이 혈장내 비타민 D3 함량에 미치는 영향
장윤환(Y . H . Chiang),원지웅(J . W . Won),강훈석(H . S . Kang),김강수(K . S . Kim),여영수(Y . S . Yeoh) 한국축산학회 1992 한국축산학회지 Vol.34 No.3
This study was conducted to elucidate the effect of different dose of 312 nm UV light on plasma vitamin D₃(D₃) content of 3 week old broiler chicks. The 0.32, 0.65 or 0.98 J/㎠ of dose was applied on dosal skin of birds and blood samples were collected day 0, 1, 2, 3, 4 and 5 after UV exposure. The plasma was isolated and D₃ concentrations were analyzed by HPLC. When dorsal skin was exposed to 0.32 J/㎠ of UV dose, the mean plasma D₃ content was 24 ng/㎖ immediately after irradiation and 46 ng/㎖ (peak) at day one after exposure. The D₃ level tended to decrease until day 5 (10 ng/㎖). As 0.65 J/㎠ of UV dose was treated, the D₃ concentrations were 20, 22, 37, 14, 29 and 10 ng/㎖ at day 0, 1, 2, 3, 4 and 5 after irradiation, respectively. When 0.98 J/㎠ dose was applied, the plasma D₃ levels were 25, 25 and 34 ng/㎖ at day 0, 1 and 2, respectively and decreased thereafter. Consequently it was shown that the treatment of 0.32 J/㎠ produced more D₃ in chick plasma than 0.65 or 0.98 J/㎠ did (P$lt;.05).
상이 (相異) 한 선량의 자외선을 조사한 브로일러 병아리에 있어서 간장 비타민 D 농도의 경시적 변화
장윤환(Y . H . Chiang),강훈석(H . S . Kang),여영수(Y . S . Yeoh),김강수(K . S . Kim),조인호(I . H . Cho),배은경(E . K . Bae) 한국축산학회 1992 한국축산학회지 Vol.34 No.5
This study was carried out to elucidate the time course variation of liver vitamin D(VD) concentration of broiler Hubbard chicks fed VD-deficient diet for 31 days in a subdued light room and exposed to UVB light(maximum intensity at 297 nm) with dose of 0.204 or 0.408 mJ/㎠. The lipid in liver was extracted by chloroform-methanol (2:1) and VD fraction was separated by Sep-Pak silica cartridge. The VD contents were analyzed by normal phase HPLC. The control birds received no UVB irradiation showed the liver VD level of 5 ng/g. When 0.204 mJ/㎠ was treated to whole body of chicks, the liver VD level tended to increase until 90 hrs after irradiation, where the maximum value of 11 ng/g appeared. A decreased level of ng/g was present at 138 hrs after exposure. The peak value (16 ng/g) was shown also at 90 hrs after UVB treatment when 0.408 mJ/㎠ was applied. A low level of 5 ng/g was present at 138 hrs after irradiation. Consequently it was verified that 0.408 mJ/㎠ of UVB treatment produced more VD in liver of broiler chicks than the 0.204 mJ/㎠ did, and the highest level of liver VD appeared at 90 hrs after exposure.
자외선의 상이한 선량이 브로일러 병아리의 중족골 광물질 함량에 미치는 영향
조인호,장윤환,여영수,강훈석,김강수,배은경 한국축산학회 1994 한국축산학회지 Vol.36 No.2
This study was carried out to investigate the concentrations of Ca, P and ash in the metatarsus of broiler chicks exposed to 0.204 mJ/㎠ 30 min) or 0.409 mJ/㎠(60 min) of medium wave ultraviolet(UVB) fight. Eighty two Hubbard dayold broiler chicks(2 doses × 9 elapsed times × 4 replicate + 10 negative controls) were fed vitamin D(VD)deficient diets for 31 days in a windowless subdued light room. The metatarsus were collected at 0, 6, 12, 18, 30, 42, 66, 90, or 138 h after UVB irradiation. The metatarsus bones were separated from adhering tissue, ether extracted, dried and ashed(A.O.A.C., 1984). The Ca content in non-fat dry metatarsus was measured by atomic absorption spectrophotometry and P by urnmonium metavanadate colorimetry. The mean Ca concentration in metatarsus of negative control birds was 21 %, and those of chicks exposed to UVB light for 30 and 60 min were 30 and 38%, respectively with the maximum values at 42 h after irradiation. Thereafter, the Ca contents decreased throughout the l38 h period of this research. The mean P level of control chicks was 8.5%, and the level peaked at 15% (30 min treatment) and 11%(60 min) at 66 h after exposure. The decreasing trend continued later than 66 h. This mean ash content of the control group was 43% with the ash contents of the 0.204 mJ/㎠(30 min) and 0.409 mJ/㎠(60 min) groups decreasing slightly until 42 h, but increasing to 46%(30 min) and 47%(60 min) at l38 h after irradiation. This verified that Ca and P contents increased first and ash amount increased later in metatarsal bones of broiler chicks when they were exposed to 0.204 and 0.409 mJ/㎠ UVB light. Since there was not a big difference between minerals in metatarsal bones of chicks exposed to 0.204 or 0.409 mJ/㎠ UVB light, it was thought that further experiments would be necessary to elucidate the optimum dose of UVB light less than 0.204 mJ/㎠.