복어중독에 의한 가사 상태에서 소생한 1예

송승찬,신진호,강석우,박경남,최호순,박근태,문희식,기춘석,이성희,윤병철,노우균,조균석,이민호 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.3

Tetrodotoxin is a neurotoxin produced by about 90 species of puffer fish and causes paralysis of central nervous system and peripheral nerves by blocking the movement of all monovalent cation. Ingestion of tetrodotoxin produces clinical manifestations such as paresthesias(within 10-45 min), vomiting, lightheadedness, salivation, muscle twitching, dysphagia, difficulty in speaking, convulsion and death that expressed by cardiopulmonary arrest with loss of brain stem reflex sometimes. Tetrodotoxin prevents or delays ischemia induced neuronal death by way of following 3 mechanisms. Firstly, it reduces the energy demand of the brain tissues. Secondly, it delays or even prevents anoxic depolarization. Finally, it deminishes ischemia induced cell swelling and cerebral edema. We report a case of puffer fish poisoning which presented with cardiopulmonary arrest and loss of brain stem reflex, but completely recovered by aggressive cardiopulmonary resuscitation.

RAPD Analysis and Antimicrobial Susceptibility of Streptococcus equi subsp. zooepidemicus Isolated from Thoroughbred Horses

Seong-kyoon Choi(최성균),Yong-soo Park(박용수),Kwang-hyun Cho(조광현),Gil-jae Cho(조길재) 한국생명과학회 2010 생명과학회지 Vol.20 No.5

Streptococcus equi subsp. zooepidemicus는 말의 생식기 질환을 유발하는 중요한 병원체 중 하나로서 국내 말의 생산성 향상을 도모할 목적으로 제주도에서 사육중인 더러브렛 암말의 유산태아 및 그 말의 자궁내용물과 장수목장에서 암말의 교배전 생식기 질환이 의심되는 말의 질 내용물로부터 시료를 채취하여 Streptococcus equi subsp. zooepidemicus을 분리하여 그 분리균의 특성 및 항균제 감수성 검사를 실시한 결과, RAPD typing에서는 크게 4개의 cluster로 구분되었다. 특히 제주도에서 분리한 균주와 장수목장에서 분리한 하나의 균주가 동일한 pattern을 나타내었다. 항균제 감수성 검사에서는 대부분의 균주가 ampicillin 등의 약제에 감수성이 있음을 확인하였다. 본 연구의 결과는 국내에서 사육중인 암말의 생식기 질병의 예방 및 치료에 유용할 것으로 생각된다. A total of 68 samples were collected including vaginal mucosa (n=66) from Jangsu stud farm, an equine aborted fetus (n=1), and uterine contents (n=1) from Jeju island. Seventeen Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) strains isolated from horses in Korea were identified as S. zooepidemicus by biochemical tests and sodA?seeI specific multiplex PCR. All isolated strains were divided into 4 clusters: group 1 (No. 2, 3, 5, 6, 7, 11, 12, 13, 14, 15), group 2 (No. 4, 9), group 3 (No. 10, 16, 17), and group 4 (No. 1, 8) by RAPD typing. In group 3, No. 10 isolate that was isolated from vaginal mucosa was indistinguishable from No. 16 and 17 isolates, which were isolated from the equine uterine contents and the equine aborted fetus, respectively. The results of this study suggest that a limited epidemiological relationship exists between the strains from Jangsu (No. 10) and Jeju (No. 16 and No. 17). All isolates showed a high susceptibility to ampicillin, cefoxitin, ceftiofur, cephalothin, florofenicol, gentamicin, nalidixic acid, oxacillin, penicillin, tiamulin, tylosin and vancomycin in antimicrobial susceptibility tests. These results may provide the basic information needed to establish strategies for the treatment and prevention of reproductive diseases in mares in Korea.

Coinfected cases with equine herpesvirus type 1, 4 and Streptococcus equi subsp. zooepidemicus in throughbred horse

( Seong Guk Kim ),( Gil Jae Cho ),( Min Hee Cho ),( Young Hoan Kim ),( Hong Young Lee ),( Jeong Hye Choi ),( Jeong Hwa Kim ),( Seong Kyoon Choi ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.2

The Thoroughbred horse was an approximately 4-years-old castrated male with highly emaciation, nasal epistaxis and subsequently died. Gross necropsy revealed epistaxis and hyperemia on the lung, multiple hemorrhage in muscle, and liver was focally attached to the peritoneum with fibrin. According to polymerase chain reaction (PCR), Equine herpes virus type 1 and 4 (EHV type 1, 4) was detected in the lung and trachea. In bacterial culture from kidney, liver, spleen, muscle and blood, Streptococcus equi subsp. zooepidemicus was isolated. Based on the gross lesion and PCR, this horse was diagnosed as EHV type 1, 4 and S. zooepidemicus coinfection.

더러브렛 씨암말의 생식기내 세균의 분포 및 항생제 감수성 양상

Seong-kyoon Choi,Soo-gil Lee,Jae-hyek Yang,Gil-jae Cho 한국임상수의학회 2007 한국임상수의학회지 Vol.24 No.1

The biochemical and molecular characteristics of Streptococcus equi subsp. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea

Choi, Seong-Kyoon,Kim, Seong-Guk,Cho, Gil-Jae The Korean Society of Veterinary Service 2011 韓國家畜衛生學會誌 Vol.34 No.3

Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is a pathogen of a variety of infections in horse. We studied biochemical and molecular characteristics of S. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea. Seventy-nine isolates were identified as S. zooepidemicus by biochemical and PCR method from 374 horses. The biochemical characteristics of S. zooepidemicus isolates were positive reaction of lactose and sorbitol. However, S. zooepidemicus isoltes were negative reaction of inulin, mannitol, raffinose, trehalose, aesculin hydrolysis, growth in 6.5% NaCl and variable reaction of maltose. Epidemiological investigations of S. zooepidemicus isolates were performed by fragment analysis of SzP (S. zooepidemicus protective protein) gene, CNE (collagen binding protein) gene and ISR (16s rRNA intergenic spacer region) gene using ABI Prism $3,130{\times}1$ Genetic Analyzer System. All isolates were shown single amplification size of 906 bp in CNE gene, but SzP and ISR gene were shown variable patterns of fragment size. The characteristics of S. zooepidemicus investigated in this study will be very useful for the prevention of infection and the studies of epidemiologic characteristics of S. zooepidemicus, causing the severe economic losses due to reproductive failures.

The biochemical and molecular characteristics of Streptococcus equi subsp. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea

( Seong Kyoon Choi ),( Seong Guk Kim ),( Gil Jae Cho ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.3

Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is a pathogen of a variety of infections in horse. We studied biochemical and molecular characteristics of S. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea. Seventy-nine isolates were identified as S. zooepidemicus by biochemical and PCR method from 374 horses. The biochemical characteristics of S. zooepidemicus isolates were positive reaction of lactose and sorbitol. However, S. zooepidemicus isoltes were negative reaction of inulin, mannitol, raffinose, trehalose, aesculin hydrolysis, growth in 6.5% NaCl and variable reaction of maltose. Epidemiological investigations of S. zooepidemicus isolates were performed by fragment analysis of SzP (S. zooepidemicus protective protein) gene, CNE (collagen binding protein) gene and ISR (16s rRNA intergenic spacer region) gene using ABI Prism 3,130 ×l Genetic Analyzer System. All isolates were shown single amplification size of 906 bp in CNE gene, but SzP and ISR gene were shown variable patterns of fragment size. The characteristics of S. zooepidemicus investigated in this study will be very useful for the prevention of infection and the studies of epidemiologic characteristics of S. zooepidemicus, causing the severe economic losses due to reproductive failures.

경북지방 환돈에서 분리한 Streptococcus suis의 생화학적 성상 및 약제감수성

최성균(Seong-kyoon Choi),김성국(Seong-guk Kim),김영환(Young-hoan Kim),최정혜(Jeong-hye Choi),조민희(Min-hee Jo),조길재(Gil-jae Cho) 한국생명과학회 2010 생명과학회지 Vol.20 No.10

Streptococcus suis는 사람과 돼지에서 다양한 감염을 유발하는 병원체이다. 2004년부터 2009년까지 경북지방 양돈장에서 병성감정 의뢰한 돼지 가검물을 대상으로 S. suis를 분리하여 생화학적 성상, 약제감수성 시험 및 PCR을 이용한 동정을 실시한 결과는 다음과 같다. 40개 농장에서 분리한 61주의 S. suis에 대하여 생화학적 성상시험 결과 모두 S. suis로 동정되었으며, VP 음성, hippurate, esculin, arginine decarboxylase 양성, lactose 분해능이 있는 것으로 나타났다. Trehalose와 starch 분해능은 비교적 높게 나타났으며 생화학적 성상에 따른 phenotype은 11형으로 나누어졌다. 항생제 감수성 시험 결과 대부분의 균주가 amoxicillin/clavulanic acid, ampicillin, cephalothin, cefoperazone, florfenicol에 높은 감수성을 나타내었지만 amikacin, kanamycin, neomycin, streptomycin, erythromycin, clindamycin, tetracycline에는 높은 내성을 나타내었다. 16S-rRNA 특이유전자 부위를 이용한 PCR 결과 공시균주 모두 304 bp에서 증폭산물이 관찰되었다. 이들 결과는 국내에서 사육되고 있는 돼지에서 S. suis 감염을 예방하는데 도움이 될 것으로 기대된다. Streptococcus suis is a worldwide pathogen of a variety of porcine infection and has also been described as a pathogen for humans. We studied biochemical characteristics, antimicrobial susceptibility, and identification of polymerase chain reaction (PCR) of S. suis isolated from diseased pigs in Gyeongbuk province from 2004 to 2009. Sixty-one isolates were identified as S. suis by biochemical characteristics and PCR from 40 farms. The biochemical characteristics of S. suis isolates were production of VP-negative, hippurate, esculin, and arginine decarboxylase-positive, and fermentation of carbohydrate was variable lactose, trehalose, inulin, and raffinose, which was typeable 11 phenotype. In an antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, ampicillin, cephalothin, cefoperazone and florfenicol, while being highly resistant to streptomycin, kanamycin, amikacin, neomycin, erythromycin, clindamycin, and tetracycline. The isolates were divided into 11 phenotypes of biochemistry. By using PCR, the 16S-rRNA gene DNA fragment was detected at 304 bp frm all of isolates. These results may provide the basic information needed to establish strategies for the prevention of S. suis infection in pigs.

더러브렛 말의 피부병변에서 분리한 Staphylococcus intermedius의 약제 감수성 양상

최성균(Seong-kyoon Choi),박청규(Cheong-kyu Park),권오덕(Oh-deog Kwon),조길재(Gil-jae Cho) 한국생명과학회 2008 생명과학회지 Vol.18 No.3

Staphylococcus intermedius(S. intermedius) has been rarely isolated from horse. In this study, we investigated the antimicrobial susceptibility patterns of S. intermedius isolated from skin lesions of Thoroughbred horse in Daegu Equestrian Association. The skin lesions were showed with dehairing and slight purulent inflammation. Bacteria were isolated from skin lesions and identified as S. intermedius by biochemical tests and MicroLog (BIOLOG, California, USA). Antimicrobial susceptibility test of S. intemedius isolates was performed with 33 antimicrobial agents (BBL, Maryland, USA) by using the agar disk diffusion method. It showed a high sensibility in the amikacin, amoxycillin/clavuramic acid, ampicillin, cefoxitin, cephalothin, chloramphenicol, ciprofloxacin, enrofloxacin, florfenicol, kanamycin, neomycin, nitrofurantoin, norfloxacin, ofloxacin, oxacillin, penicillin G, tetracycline and vancomycin. The horse was treated with penicillin, and cured completely after two weeks. The present results showed valuable information for treatment and prevention of skin disease in horse.

Coinfected cases with equine herpesvirus type 1, 4 and Streptococcus equi subsp. zooepidemicus in throughbred horse

Kim, Seong-Guk,Cho, Gil-Jae,Cho, Min-Hee,Kim, Young-Hoan,Lee, Hong-Young,Choi, Jeong-Hye,Kim, Jeong-Hwa,Choi, Seong-Kyoon The Korean Society of Veterinary Service 2011 韓國家畜衛生學會誌 Vol.34 No.2

The Thoroughbred horse was an approximately 4-years-old castrated male with highly emaciation, nasal epistaxis and subsequently died. Gross necropsy revealed epistaxis and hyperemia on the lung, multiple hemorrhage in muscle, and liver was focally attached to the peritoneum with fibrin. According to polymerase chain reaction (PCR), Equine herpes virus type 1 and 4 (EHV type 1, 4) was detected in the lung and trachea. In bacterial culture from kidney, liver, spleen, muscle and blood, Streptococcus equi subsp. zooepidemicus was isolated. Based on the gross lesion and PCR, this horse was diagnosed as EHV type 1, 4 and S. zooepidemicus coinfection.

MLVA를 이용한 경북지역 소에서 분리된 Brucella abortus의 유전형별

김성국 ( Seong Guk Kim ),김영환 ( Young Hoan Kim ),이홍영 ( Hong Young Lee ),최정혜 ( Jeong Hye Choi ),최성균 ( Seong Kyoon Choi ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.3

Brucella (B.) abortus is a facultative intracellular pathogen that infects a wide variety of animal species and human. Brucellosis is the zoonosis and an extremely important disease around the world. Although the eight species can be differentiated by conventional phenotypic tests, these species display a high degree of DNA homology in DNA-DNA hybridization assay (>90%). Various methods have been established for genotyping of Brucella species, but most of analytical methods are lack reproducibility and limited capability to differentiate them. The attempt of this study was to evaluate multiple- locus VNTR analysis (MLVA) for use of epidemiological trace-back analysis in bovine brucellosis. Ninety-four B. abortus isolates from Gyeongbuk province during 2006~2010 were analyzed using 16 VNTR locus. High resolution automatic capillary electrophoresis system was used for more throughput, simpleer, faster, and better discriminable than conventional gel electrophoresis. As a result, 13 different genotypes were identified from 94 B. abortus isolates. MLVA could contribute to epidemiological trace-back analysis of bovine brucellosis.