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핵이식을 통한 양서류 역위배세포의 발생수행능력에 관한 연구
정해문,이자경,김덕희 한국유전학회 1987 Genes & Genomics Vol.9 No.1
In order to investigate the mechanism of early amphibian embryogenesis, the uncleaved eggs of Korean anuran Rana dybowskii were inverted at various times as well various duration after fertilization. However, developmental arrest at early gastrula stage always followed partial invagination of the dorsal lip. Nuclear transplantation was then employed to reveal the cause of death. Comparing the developmental capacity of the endodermal cells of inverted R. dybowskii and those of non-inverted normal control embryos, there was no difference between those two cases. It was concluded that the death of the inverted embryos was not attributed to the endodermal cells and the cause should be found elsewhere. Nuclear transplantations were also carried out to investigate the extent of development by newly injected nuclei from normal embryos. For that purpose nuclei from the cells at various developmental stages of blastula, gastrula, neurula and tail bud stages were injected into the eggs whose nuclei had been previously eliminated. It was revealed that as cells differentiated their nuclei progressively restricted in their ability to promote the development of enucleated eggs. The results are quite similar to those early observations by other investigators who used other amphibian species.
Partial Cloning of Xhox3.1 Gene in Xenopus laevis
정해문,김태균,남상욱,고제명 한국유전학회 1996 Genes & Genomics Vol.18 No.2
VNTR(variable number tandem repeat) loci in the genome are characterized-as their copy number of the same repeat unit and are extremely polymorphic having potentially hundreds of alleles at a single locus. In this study, allele distribution on the hTPO VNTR marker was analyzed by PCR(polymerase chain reaction) in Korean population(100 individuals). Twenty two alleles were identified in the hTPO VNTR locus and alleles 7(8.5%), 10(11.0%), and 15(15.0%) showed three highest peaks. The allele frequency of hTPO VNTR locus is in the 95% confidence interval. PIC(polymorphism information content) value calculated on hTPO VNTR loci was 0.92. POE(power of exclusion) value used in paternity cases was 0.80. MP(matching probability) used in forensic identification was 0.018. In conclusion, statistical data from hTPO VNTR locus showed this locus is a very useful genetic marker for the linkage study, evolutionary genetics and forensic application.
멕시코산 Axolotl , Ambystoma mexicanum 의 FGF - 8 cDNA 절편 클로닝
정해문,남상욱,김원선,한만종 한국유전학회 1997 Genes & Genomics Vol.19 No.2
FGF-8 (fibroblast growth factor-8) has been known to play crucial roles in various developmental processes such as initiation, outgrowth and patterning of vertebrate limbs, especially in amniotes. However, it is not known whether FGF-8 plays similar roles in the developmental processes of amphibians. Here, we report a partial fragment cloning of an FGF-8 cDNA from embryo and limb blastema cDNA libraries of a salamander, Mexican axolotl (Ambystoma mexicanum). The 1.27 kb cDNA fragment cloned in the present study contains a coding region covering 192 amino acid residues with conserved consensus amino acid residues of FGF family and shows 85% amino acid identity with that of mouse, and 91% amino acid identity with those of chick and Xenopus. Therefore, the cDNA clone obtained in the present study is believed to be a partial fragment of a putative FGF-8 cDNA of Mexican axolotl.