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대전 지역의 공단 폐수에서 분리한 수은과 카드뮴 내성 세균의 분리 및 특성
유경만,전희근,박준봉,이인수,박진숙,하남주,박용근 한국환경과학회 1997 한국환경과학회지 Vol.6 No.3
Mercury- and cadmium-resistant bacteria were isolated from an industrial complex wastewater of Taejon area. All of them were motile, gram negative rods. In the results of physicochemical test and VITEK card test, HMI was identified with Acinetobacter calcoaceticus, CM3 was identified with Comamonas acidovorans, HM2, HM3, CM1, and CM4 were Pseudomonas sp., but HM4 and CM2 were unidentified. They were tested for susceptibility to 14 heavy metals. Mercury-resistant bacteria(HM1, HM2, HM3, and HM4) were sensitive to low concentration(100∼400ppm) of Cd^2+, Co^2+, Zn^2+, and Ni^2+ while cadmium-resistant bacteria(CM1, CM2, CM3, and CM4) showed resistance up to the high concentration(600∼1,200ppm) of these metal ions. As a result of resistance spectrum test of mercury-resistant bacteria, HM1 was broad-spectrum strain, HM2, HM3, and HM4 were narrow-spectrum strains. Transmission electron microscopic examination of cell wall of HM1 culture grown with and without 100ppm of HgCl_2 showed remarkably morphological abnormalities. In the result of atomic absorption spectrometric analysis of cadmium-resistant bacteria grown at 200ppm of CdCl_2 for 6h, all of them accumulated cadmium(14ppm∼57ppm) in cell. In cadmium-resistant bacteria, CM1, CM2, and CM4 were spared from the inhibitory effect of Cd^2+ by the addition of Mn^2+, CM4 were also spared from the inhibitory effect of Cd^2+ by the addition of Mn^2+ as well as Zn^2+.