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김송이,이혜존,봉성용,김정호,이종희,조상래,김영삼 대한결핵 및 호흡기학회 2016 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.121 No.-
Tuberculosis (TB) remains a major infectious cause of mortality and morbidity, with approximately 8.6 million new cases and 1.3 million deaths per every year (WHO 2015). Innovative efforts have been made to meet the global targets on tuberculosis control. In order to evaluate novel development on vaccines and drugs, it is essential to have effective surrogate markers of risk and protection against TB in advance. Especially in preparation of clinical trials on new TB drugs or therapeutic vaccines, monitoring tools should be ready to predict the progression outcomes of treatment in TB patients. Culture conversion at 2 months of drug treatment has been used as a predictive index for drug treatment response in patients. However, the result of culture is not always available in a resource-limited setting. Interferon-γ-induced protein 10 kDa (IP-10) is a pro-inflammatory chemokine produced by monocytes and macrophages that traffics Th1 lymphocytes to inflamed foci. Previous studies showed that measurement of IP-10 in urine can be useful to monitor the progress of treatment. In this study, we evaluated the level of IP-10 in a serial urine samples from patients with active TB. The data showed that levels of IP-10 in urine significantly increased after 2-month of treatment but decreased by the completion of treatment (P < 0.01). In conclusion, unstimulated IP-10 in urine of patients can be used to monitor the progression of treatment in TB patients.
P-95 Utility of microneedle, MicronJet 600<sup>TM</sup>, for BCG vaccination
김정호,봉성용,김수현,조상래,이혜존 대한결핵 및 호흡기학회 2016 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.121 No.-
Bacille Calmette-Guerin (BCG) is the only licensed vaccine against tuberculosis (TB) in the world. The conventional route of BCG vaccination is an intradermal administration with needle and syringe, which requires a well-trained nurse with experience. To complement any complexity of technique, a novel microneedle device has been introduced to deliver vaccines and biologics. The MicronJet 600TM (NanoPass Technologies Ltd, Nes Ziona, Israel) is composed of three microneedles, 0.6 mm in length, enabling controlled delivery depths with minimal pain and lowered risk associated with handling needles during injection. In this study we aimed to evaluate the microneedle device in terms of recovery rate of live BCG bacilli. The recovery rates of BCG vaccine with microneedle and conventional needle were 141.7% and 96.3%, respectively with no significant difference. The result showed live BCG bacilli can get through microneedle with 0.6 mm without being clogged. In conclusion, clinical evaluation of MicronJet 600TM should be succeeding for BCG vaccination, which expects to be safer, easier, and simper than intradermal injection using conventional needle.
봉성용,김정호,이종희,이혜존,조상래 대한결핵 및 호흡기학회 2016 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.121 No.-
The Interferon-γ Release Assay (IGRA) has been widely used for the diagnosis of latent TB infection (LTBI) measuring soluble IFN-γ production induced by stimulation with TB specific antigens. Despite the fact that LTBI can be diagnosed by the commercial IGRA tests in a more specific and faster manner than in vivo Tuberculin Skin Test, the high cost of these tests has been hindered from wider utility to the public. Various efforts have been made to reduce the cost of diagnosis of LTBI with equivalent efficacy of the assay. In this study, we evaluated the made-to-order IFN-γ ELISA kit in terms of reproducibility and consistency. The data showed that the coefficient of variance (% CV) of repeatability and intermediate precision was 15.583 % and 6.478 %, respectively. The accuracy of the in-house ELISA kit was 104.9 % (SD ± 10.52). In addition, we evaluated the in-house IFN-γ ELISA kit for the diagnosis of LTBI using the clinical supernatants form QuantiFERON-TB Gold In-Tube (QFT-GIT). The results showed the excellent agreement between commercial QFT-ELISA kit and in-house ELISA kit (99%, Kappa coefficient = 0.98, P <0.001). In conclusion, the in-house ELISA kit showed reproducible and consistent results. And the results from the in-house ELISA kit was concordance to that from the commercial ELISA kit (QFT-ELISA kit) for the diagnosis of LTBI.