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오병근,YongTae Kim,Ju-Won Jeon,Tae-Hwan Kim,Eun-Sol Lee,Myeong-Jun Lee 나노기술연구협의회 2019 Nano Convergence Vol.6 No.37
Many methods for early diagnosis of the disease use biomarker tests, which measure indicators of biological state in body fluids or blood. However, a limitation of these methods is their low sensitivity to biomarkers. In this study, human serum albumin (HSA) based nanoparticles capable of encapsulating excess horseradish peroxidase (HRP) are synthesized and applied to the development of enzyme-linked immunosorbent assay (ELISA) kit with ultra-high sensitivity. The size of the nanoparticles and the amount of encapsulated enzyme are controlled by varying the synthesis conditions of pH and protein concentration, and the surface of the nanoparticles is modified with protein A (proA) to immobilize antibodies to the nanoparticles by self-assembly. Using the synthesized nanoparticles, the biomarker of breast cancer, thioredoxin-1, can be measured in the range of 10 fM to 100 pM by direct sandwich ELISA, which is 105 times more sensitive than conventional methods.
오병근,조남효 한국군사과학기술학회 2000 한국군사과학기술학회지 Vol.3 No.2
This paper describes modeling and simulation of automotive forward collision warning and avoidance system using CASE(Computer-Aided Systems Engineering) tool. The system is composed or many sensors, a controller, warning devices, brakes and etc. The system was modeled by one activity chart, fourteen state charts and one module chart. Rear-end collision scenarios was generated by Simulink and used to support Stalemate model. The resulting model was used to evaluate the correctness of function and behavior of the system. A simulator for the system has been designed and used to validate the model under realistic operating conditions in the laboratory. To model and simulate the system's functionality and behavior brings clarity to system design early in the system development.