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노환국,김완영,김길현,Nho, W.G.,Kim, W.Y.,Kim, K.H. 국립한국농수산대학교 교육개발센터 2008 현장농업연구지 = Journal of practical agricultural resear Vol.10 No.1
For the prevention of Hanwoo calf diarrhea, the floor in a cattle shed was flamed using liquefied petroleum gas (LPG) in an attempt to substitute for the chemical disinfection method. Before flaming, litter on the floor was removed and cleaned. Outbreak rate of diarrhea in calves vaccinated with ScourGuard 3K<sup>®</sup> was decreased from 70% to 30% and the effect of the flame disinfection lasted for three to four months.
노환국,이장형,Nho, Whan-Gook,Lee, Jang-Hyung 국립한국농수산대학교 교육개발센터 2004 현장농업연구지 = Journal of practical agricultural resear Vol.6 No.1
The method of insertion of T-type cannula into the proximal duodenum of cattle was established for the feed digestibility test. Five cattle were anesthetized with rumpun and 2% lidocaine. The incision(15 ~ 20 cm) through the abdominal wall exposing the peritoneal cavity was made. The identified duodenum was extracted through the abdominal incision. The cannula was inserted into the incised duodenal wall. Cannula barrel was extracted between the 10th and 11th rib. All of operated cattle were healthy and cannula remained completely functional until 20 months after a proximal duodenal cannulation.
노환국,서성,김민경,서건식,Nho, W.G.,Seo, S.,Kim, M.K.,Seo, G.S. 국립한국농수산대학교 교육개발센터 2012 현장농업연구지 = Journal of practical agricultural resear Vol.14 No.1
To elucidate the mycotoxin production of Penicillium, Aspergillus and Fusarium spp. isolated from round bale silage, TLC analysis of culture filtrates were conducted. Mycotoxin citrin and patulin were detected from culture filtrates of Penicillium paneum. Aflatoxin was detected from culture filtrates of Aspergillus flavus. Gliotoxin are known to produce by A. fumigatus was not detected. Mycotoxins produces by Fusarium spp., Fumonisin, zearalenone and deoxynivalenol was not detected in the culture filtrates of Fusarium proliferatum.
노환국,이장형,김완영,여준모,Nho, W.G.,Lee, J.H.,Kim, W.Y.,Yeo, J.M. 국립한국농수산대학교 교육개발센터 2008 현장농업연구지 = Journal of practical agricultural resear Vol.10 No.1
Gnotobiotic piglets were routinely produced by hysterectomy. In this study, 22 pregnant miniature pigs (111th to 113th day of gestation) were used for hysterectomy. Before surgery, 14 pigs were insensibilizated by Ketamine 50<sup>®</sup> plus CO<sub>2</sub> gas and 8 pigs by a slaughter pistol. The high level of Ketamine 50<sup>®</sup> (0.09㎖/kg) was faster (146 vs 283 seconds) in surgery but the time taken for complete revival of one piglet was more prolonged (427 vs 64 seconds) than 0.03㎖/kg level. In hysterectomies with a slaughter pistol, surgery time was faster (470 vs 155 seconds) and the rate of alive piglets was higher (97.0 vs 83.8%) than in those with Ketamine 50<sup>®</sup>. There were no problems in the rate of alive newborn piglets even when sows were hysterectomized at 3 days prepartum.
노환국,여준모,김완영,이장형,서성,김민경,서건식,Nho, W.G.,Yeo, J.M.,Kim, W.Y.,Lee, J.H.,Seo, S.,Kim, M.K.,Seo, G.S. 국립한국농수산대학교 교육개발센터 2012 현장농업연구지 = Journal of practical agricultural resear Vol.14 No.1
To identification of fungi that occurs round bale silages, 253 fungal contaminated samples were collected from 2009 to 2011. Total 253 silage samples from Italian ryegrass, sudan grass, rye, corn, barley and oat were analysed. Total 270 strains were purely isolated from contaminated round bale silages. The fungi were identified with morphological characteristics and rDNA sequence analysis. Nineteen species of fungi(Rhizopus sp., Fusarium spp., Coprinus sp., Blastomyces sp., Aureobasidium sp., Polypaecilum sp., Botryoderma sp., Mucor sp., Scytalidium sp., Sphaeropsis sp., Aspergillus spp., Trichocladium sp., Humicola sp., Staphylotrichum sp., Periconia sp., Verticillium sp., Diplococcium sp., Penicillium spp. and Trichoderma spp.) were identified by morphological characteristics. On the other hand, fungi isolated from silage were identified to Acremonium strictum, Aspergillus tubingensis, Bionectria ochroleuca, Dipodascaceae sp., Fusarium proliferatum, Fusarium oxysporum, Fusrium solani, Gelasinospora reticulata, Gibberella moniliformis, Gibberella zeae, Nectria mauritiicola, Penicillium paneum, Pseudallecheria boydii, Schizophyllum commune, Scopulariopsis brevicaulis and Simplicillium lamellicola by rDNA sequence analysis. Penicillium sp. and Trichoderma sp., were isolated 74 and 64 strains, respectively. Humicola sp., Aspergillus sp., Coprinus sp., and Fusarium spp. were identified 10 to 30 strains. Most fungi were isolated together with more than one species in a sample looked like one species with the naked eyes.
Multiplex RT-PCR 기법을 이용한 소의 로타바이러스, 코로나바이러스 및 설사병바이러스의 동시진단
노환국,이장형,Nho, W.G.,Lee, J.H. 국립한국농수산대학교 교육개발센터 2003 현장농업연구지 = Journal of practical agricultural resear Vol.5 No.1
The bovine rotavirus(BRV), bovine coronavirus(BCV) and bovine viral diarrhea virus(BVDV) are main viruses of bovine viral diarrhea disease. These viruses could be rapidly amplified by the reverse transcriptase polymerase chain reaction(RT-PCR). This study was conducted to develop rapid and accurate diagnostic methods of these viral diseases by multiplex RT-PCR. Specific primers were designed based on the sequences reported by Chang KO et. al. (1997) and Schroeder BA, et. al. (1990), RNA were prepared from the cultured viruses, first-stranded DNAs were synthesised by reverse transcriptase. PCR were conducted to amplify specific regions of the viruses by multiplex. Three bands such as 1,062bp for BRV, 458bp for BCV, and 300bp for BVDV were successfully produced by multiplex RT-PCR. In conclusion, this result suggested that these viruses could be diagnosed rapidly and accurately by multiplex RT-PCR.
Indirect Immunoperoxidase 법을 이용한 조직내 뉴켓슬병 바이러스 항원동정
노환국,서정향,김순복,Nho, Whan-goog,Sur, Jung-hyang,Kim, Soon-bok 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.3
The present experiment was done to identify newcastle disease virus(NDV) antigens in frozen sections of various oragns from experimentally NDV-infected with indirect immunoperoxidase method. Section were incubated with rabbit anti-NDV polyclonal as first antibody, followed by incubation with goat anti-rabbit or protein A peroxidase conjugate. Positive reactions were often detected in the epithelium of trachea and in the lymphocyte of spleen at 24 hours after virus inoculation. the viral antigen was localized mainly in the cytoplasm of infected cells. The method approved to be highly specific for the identification of NDV and allowed a precise localization of the viral antigens in infected cells.