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김영호,김이슬,김동흔,김세연,서경진,신수현,이재영,김영호 한국곤충학회 2019 Entomological Research Vol.49 No.6
Drosophila melanogaster is attracted to chemicals produced by fermentation and it is abundantly found in rotten fruits. Considering its habitat, the fruit fly is reported to be tolerant to environmental chemicals. Quantitative real-time polymerase chain reaction was employed to investigate the expression pattern and physiological function of genes putatively involved in chemical detoxification. In quantitative real-time polymerase chain reaction assays, normalization of target gene expression with internal reference genes is required. These reference genes should be stably expressed during chemical exposure and in chemical-free conditions. In this study, therefore, we used two programs (geNorm and BestKeeper) to evaluate the expression stability of five reference genes (nd, rpL18, ef1β, hsp22 and tbp) in female adult flies exposed to various concentrations of methanol and ethyl acetate. Four genes (nd, rpL18, ef1β and tbp) were found to be suitable for use as reference genes in methanol-treated flies and three genes (ef1β, nd, tbp) were found to be suitable for use as reference genes in ethyl acetate-treated flies. These results suggested that a combination of two genes among these stably expressed genes can be used for accurate normalization of target gene expression in quantitative real-time polymerase chain reaction-based determination of gene expression profiles in D. melanogaster treated with both chemicals.
세미나 - 일본인쇄산업연합회 Gravure인쇄 Service(연포장) Green 기준 Guide Line
김영호 (사)한국포장협회 2011 包裝界 Vol.214 No.-
한국포장기술인협의회는 지난 해 10월 22일 서울 팔래스호텔 로얄볼룸에서 제26회 한국포장기술인 세미나를 개최했다. 이날 세미나는 '라미네이트용 수성접착제 기술과 적용 방법 및 일본인쇄산업연합회의 Gravure인쇄 Service(연포장) Green 기준 가이드 라인'을 주제로 진행됐다. 본 원고는 세미나에서 김영호 한국포장기술인협의회 회장이 발제한 자료로, 지난 1월호에 이어 인쇄 Service Green 기준과 Green Printing 인정제도, Gravure 인쇄 Service(연포장) Green 기준 및 해설에 대해 살펴보도록 한다.
다양한 조건의 살충제에 노출된 꿀벌에서의 qRT-PCR을 위한 안정 발현 reference 유전자 선정
김영호,김영호 한국양봉학회 2023 韓國養蜂學會誌 Vol.38 No.1
Pesticides have been considered to be a major factor that leads to a significant decline in honey bee populations, including colony collapse disorder. To understand pesticide detoxification physiology in honey bees, it is essential to identify the gene expression in different pesticideexposed conditions. For the accurate determination of target gene quantification through quantitative real-time polymerase chain reaction (qRT-PCR), reference gene stably expressing across different samples should be selected. Therefore, in this study, to select the optimal reference genes, we analyzed the amplification stability of five candidate reference genes (RPS5, RPS18, GAPDH, ARF1, and RAB1A) from honey bees exposed to seven pesticides (acetamiprid, imidacloprid, flupyradifurone, fenitrothion, carbaryl, amitraz, and bifenthrin) across various condition of samples, including different tissues and exposure time and concentration of pesticides using four programs (geNorm, NormFinder, BestKeeper, and RefFinder). Although the stability values of genes varied depending on different analysis algorithms, our results suggest that RPS5 is the most appropriate reference gene for the identification of target gene expression levels in qRT-PCR assays for honeybees under various conditions of pesticide treatment.
세미나 - 일본인쇄산업연합회 Gravure인쇄 Service(연포장) Green 기준 Guide Line
김영호,Kim, Yeong-Ho (사)한국포장협회 2011 包裝界 Vol.213 No.-
한국포장기술인협의회는 지난 해 10월 22일 서울 팔래스호텔 로얄볼룸에서 제26회 한국포장기술인 세미나를 개최했다. 이날 세미나는 '라미네이트용 수성접착제 기술과 적용 방법 및 일본인쇄산업연합회의 Gravure인쇄 Service(연포장) Green 기준 가이드 라인'을 주제로 진행됐다. 본 원고는 세미나에서 김영호 한국포장기술인협의회 회장이 발제한 자료로, 인쇄 Service Green 기준과 Green Printing 인정제도, Gravure 인쇄 Service(연포장) Green 기준 및 해설에 대해 상세히 설명하고 있다.