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한국인 Actinobacillus actinomycetemcomitans 균주의 특이 독성 clone형과 혈청형 및 백혈구독성과의 관계
구영,Ku, Young 대한치주과학회 1995 Journal of Periodontal & Implant Science Vol.25 No.3
Previous studies have demonstrated that not all A. actinomycetemcomitans produced significant level of leukotoxic factor and its leukotoxicity have associated with serotype and genetic variation. Our aim was to investigate on the interrelationship between serotype and leukotoxicity of an A. actinomycetemcomitans consisting of 13 clinically well characterized. Korean isolates and to evaluate if particular virulent clonal types of A. actinomycetemcomitans are associated with periodontal disease. For this study, 13 strains of A. actinomycetemcomitans from 6 patients with periodontal disease were isolated and identified by using a selective medium(tryptic soy agar supplemented with 10% serum, $75{\mu}g$ of bacitracin and $5{\mu}g$ of vancomycin per ml) in 10% C02 incubator for 3days with routine Gram staining, colony morphology and biochemical test..For serotyping, antisera were prepared from reference strains of 5 serotypes. (ATCC 29523,Y4, SUNY aB 67, IDH 781, IDH 1705) and then ammonium sulfate precipitation, immunoabsorption and indirect immunofluoroscent procedures were done. For analysis of leukotoxicity, sonic extract of A. actinomycetemcomitans exposed to PMN, and trypan blue was stained for counting the cell viability. Finally Southern blot analyses of genomic DNA digested with the restriction enzyme Tag I was done and the Southern blots were hybridized with the 530bp fragment, termed delta 530, originating from the ltx promoter of strain 652 and deleted from strain JP2. Also ltxA-3.1 and SC2 probe from strain JP2 were hybridized with genomic DNA fragments. Results reveal that strains isolated showed approximately equal proportions of 3 serotypes(b, d, e) and serotype b was not detected. 2 patients harbored 2 different serotypes in the same disease site. The prevalence of leukotoxic strain was 23% and there was no relationship between serotype, leukotoxicity and clinical observations. Especially virulent clonal types of Actinobacillus actinomycetemcomitan (JP2 strain) could not found. Further studies are necessary on the genetic polymorphism of leukotoxin and its relations to clinical status.
혈소판유래 성장인자 함유 흡수성 차폐막이 치주조직의 재생에 미치는 영향
구영,김정은,한수부,정종평,박윤정,이승진,권영혁,Ku, Young,Kim, Jeong-Eun,Han, Soo-Boo,Chung, Chong-Pyoung,Park, Yoon-Jeong,Lee, Seung-Jin,Kwon, Youg-Hyuk 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.1
PDGF-BB has been recognized as a highly potential growth factor for guided tissue regeneration in periodontal defect. This study carried out histologic and histometric evaluation of $200ng/cm^2$ PDGF-BB loaded bioresorbable membrane made from polyglycolic and polylactic acid. It was tested for its biocompatibility, ability to prevent epithelial downgrowth and amount of periodontal regeneration. Without membrane and PDGF-BB unloaded bioresorbable membrane were used as control. Healthy six beagle dogs were used. Each dog was anesthetized and buccal flaps were reflected in the mandibular and maxillary premolar areas. Buccal alveolar bone between the mesiobuccal and distobuccal line angles was surgically removed on the lower 2nd and 4th premolar in mandible, 2nd premolar in maxilla, to a level 4mm apical to the cementoenamel junction with creating a Class II buccal furcation defect for available space. Care was taken not to remove the root cementum layer and rubber impression materials were placed over each surgically created defect. Flaps were repositioned and sutured. Reconstructive surgery was performed 1 month after defect preparation. PDGF-BB loaded membranes and controls were randomly placed on maxillary 2nd premolars and mandibular 2nd and 4th premolars. Plaque control regimen was instituted with daily brushing with a 0.1% chlorhexidine digluconate during experimental periods. The animals were sacrificed 2 and 5 weeks after surgery and undecalcified specimens were prepared for histologic evaluation. The degree of coronal regrowth of new bone, new cementum and the amonut of new bone areas formed on the defected area of the PDGF-BB loaded membrnae turned superior to without membrane and drug unloaded membrane. Experimental membrane could prevent the epithelial downgrowth irrespective of drug loaded or not and showed good biocompatiblity, These results implicated that PDGF-BB loaded bioresorbable membrane could be highly useful tool for guided tissue regeneration of periodontal defects.
이온 빔 조사 처리된 키토산 스펀지의 세포적합도에 관한 연구
구영,Ku, Young 대한치주과학회 1998 Journal of Periodontal & Implant Science Vol.28 No.2
Chitosan is a biodegradable and non-toxic material with a molecular weight of 800-1,500Kd which can be obtained in various forms with extraordinary chemical structures and biological characteristics of which enables it to be used in many fields as a biomaterial. Ion irradiation is a useful tool to modify chemical structures and physical properties of high molecular weight polymers. The basic hypothesis of this study is that when surface properties of chitosan in a sponge form are modified with ion beam-irradiation and cell adhesion properties of chitosan would improve and thereby increase the regenerative ability of the damaged bone. The purpose of this study was to illuminate the changes in the cytocompatibility of chitosan sponges after ion beam-irradiation as a preliminary research. Argon($Ar^+$) ions were irradiated at doses of $5{\times}10^{13}$, $5{\times}10^{15}$ at 35 keV on surfaces of each sponges. Cell adhesion and activity of alkaline phosphatases were studied using rat fetal osteoblasts. The results of this study show hat ion beam-irradiation at optimal doses($5{\times}10^^{13}\;Ar^+\;ion/cm^2$) is a useful method to improve cytocompatibility without sacrificing cell viability and any changing cell phenotypes. These results show that ion beam-irradiated chitosan sponges can be further applied as carriers in tissue engineering and as bone filling materials.
월드프렌즈코리아(WFK) 중장기 전략 수립을 위한 해외봉사 성과 측정 동향 분석
구영은,최경화 한국국제협력단 2017 국제개발협력 Vol.2017 No.1
2016년 지속가능개발목표(SDGs)의 세부 목표와 지표가 합의되고, 봉사활동이 SDGs 이행에 강력하고 범분야적인 수단임이 강조된 현 시점에서, 해외봉사를 통한 SDGs 달성 성과의 증명은 점점 중요해질 추세이다. 이러한 추세에 맞추어 전 세계해외봉사 파견기관 및 관련 학계는 해외봉사의 성과 측정에 대한 연구에 박차를 가하고 있다. 그러나 현재까지 해외봉사 성과 증명의 방법론에 있어서 전 세계적 합의가 도출되지 않은 실정이며, 이는 바로 전세계 다양한 해외봉사 프로그램의 목적, 형태, 규모, 예산 등에 있어서 큰 차이가 있기 때문이라고 할 수 있다. 실제로 각국의 각기 다른 해외봉사 파견조직이 수행한 성과 평가 연구는 그 목적, 성과 지표, 보고 방법, 평가의 품질 등에 있어서 차이가 있기 때문에 조직별, 프로그램별로 체계적인 성과 비교조차 사실상 불가능한 상황이다. 따라서 현재 해외봉사 성과 측정에 관련된 국제적 논의와 도전과제를 검토하고 분석하여, WFK 사업에 적용 가능한 성과 평가의 중장기 전략을 모색하는 작업이 필요한 시점이라 할 수 있다. 금번 연구에서는 해외봉사의 성과 측정에 대한 동향을 해외 최신문헌 검토를 통하여 분석해보고자 하며, 그 분석 결과를 바탕으로 WFK 사업을 SDGs와 연계한 성과 평가 중장기 전략 수립 방향을 모색하고자 한다.