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姜昌禧 제주대학교 1992 논문집 Vol.35 No.-
1-Phenyl-3-methyl-4-(2-hydroxy-5-nitrophenylazo)-5-pyrazolone was synthesized, which was reacted with Ni(Ⅱ), Cu(Ⅱ), Co(Ⅲ) and Cr(Ⅲ) to form the chelate compounds. The liquid chromatographic behaviors of these metal-1-phenyl-3-methyl-4-(2-hydroxy-5-nitrophenylazo)-5-pyrazolone chelates have been stuied by the Reverse Phase High Performance Liquid Chromatography. These four kinds of chelates were successfully separated on NOVA-PAK C?? column using methanol/water(63/37) mixture as a mobile phase. It was found that the chelates were eluted properly in an acceptable range of the capacity factor value (0≤log k'≤1). The dependence of the capacity factor (log k') on the volume fraction of water in the binary mobile phase as well as on the column temperature and the enthalpy change(??H) showed a good linearity. Also, there was a good linear dependence of the capacity factor (log k') on the liquid-liquid extraction distribution ratio(D??) in methanol-water/n-pentadecane extraction system by the batch method. It suggested that the retention of the chelates in the reverse phase liquid chromatographic system was largely due to the hydrophobic effect.
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강창희,김원형 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.2
The atmospheric aerosols have been collected with a high volume tape sampler installed at the 1100m Site located at the Halla mountain from August, 1996 to May, 1999. The compositions of water-soluble components have been analyzed in order to investigate the variations of aerosol compositions and the characteristics of aerosols. The mean concentrations of water-soluble components have been found in the order of SO₄^(2-)>NH₄^(+)>NO₃^(-)>Ca^(2+)>Na^(+)>K^(+)>Mg^(2+), and the compositions of SO₄^(2-), NH₄^(+), NO₃^(-) were 60 % , 15 % , 10 % , respectively. From the investigation af the sea salt enrichment factors, the SO₄^(2-), Ca^(2+), K^(+) components were thought to be introduced into the atmospheric aerosols due to the non-sea salt effect, on the other hand, Mg^(2+) and Cl^(-) were introduced due to the sea salt effect. From the investigation of the soil enrichment factors, the SO₄^(2-), NO₃^(-), Cl^(-) and NH₄^(+) were thought to be introduced into the atmospheric aerosols through the non-soil pathways, but Mg^(2+) and and K^(+) were found to be mainly from the soil sources. Based on the factor analysis by SPSS program, the emission sources were found to be anthropogenic sources, sea salt and soil, respectively. The concentration variation of aerosol components as a function of wind direction was investigated. From the result, most of all the components were increased in the northwest direction rather than the southeast direction, and especially, the concentrations of Ca^(2+) and NO₃^(-) were increased about three times and twice respectively in the northwest wind direction.
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강창희,김원형,정덕상,변경희 濟州大學校 基礎科學硏究所 2000 基礎科學硏究 Vol.13 No.1
The rainwater samples were collected from the 1100 Site of Mt. Halla and Cheju city during the period of March in 1997 to August in 1998, and the major soluble ions were analyzed and their analytical confidence has been investigated. The volume weighted mean values of pH in 1100 Site and Cheju city were 487 and 4.82, respectively, and those of electrical conductivity were 16.0 and 23.3 μS/cm. The mean concentrations of ions in 1100 Site were in the order of Cl^(-)>Na^(+)>SO₄^(2-)>NH₄^(+)>H^(+)>NO₃^(-)>Mg^(2+)>Ca^(2+)>K^(2+)>HCOO^(-)>CH₃COO^(-)>F^(-)>HCO₃^(-)>CH₃SO₃^(-), and those in Cheju city were the same order except that NO₃^(-) was higher than H^(+). The comparison between several correlation coefficients has been applied for the investigation of analytical confidence, such as equivalent concentrations sum, the measured and calculated conductivity, and the acid fraction. The correlation coefficients between cation and anion equivalent concentrations sum were 0.981 and 0.960, respectively, in 1100 Site and Cheju city, and those of the calculated and measured electric conductivity in two regions were 0.989 and 0.993, respectively, so that the analytical data has been shown to be in good reliabilities. Also, the correlation coefficients between the acid fractions from the equivalent concentrations of ions and those from the measured pH and electric conductivity were 0.966 and 0.936, respectively, in two areas, so that these results also have shown a quite satisfactory analytical confidence.
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1999-2000년 제주시 지역 강수의 유기산 함량 분석
강창희,김원형 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.1
The precipitation samples at Cheju city were collected during the period of January in 1999 to December in 2000, and the organic acids as well as the major soluble ions were analyzed. The confidence of analytical data was confirmed by using the comparison methods such as ion-balance, electric conductivity and acid fraction, all of whose correlation coefficients were over 0.97. The ionic strengths lower than 10^(-4)M, the basis for the pure rainwater, were 46% and 30% in 1999 and 2000 respectively. The acidity contribution was mostly by SO₄^(2-) and NO₃^(-) with 90% in 1999 and 93% in 2000, and the organic acids have contributed to the acidity with only 4%. The neutralization factors by NH₃were about 0.48 and 0.34 in 1999 and 2000, whereas those by CaC0₃ were 0.22 and 0.26 respectively. The sources of the precipitation components in Cheju city were also studied with a factor analyzing method. The most probable factors were found to be anthropogenic and soil-sourced, and the second influencing factor was to be oceanic.
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강창희,김원형,홍상범,이기호,홍민선,심상규 濟州大學校 基礎科學硏究所 2000 基礎科學硏究 Vol.13 No.1
The rainwater samples were collected from the 1100 Site of hlt. Halla and Cheju city during the period of March in 1997 to August in 1998. and the major soluble ions were analyzed. The confidence of analytical data was confirmed by using the comparison methods such as ion-balance, electric conductivity and acid fraction. all of which correlation coefficients were above 0.94. The ionic strengths lower than 10^(-4)M, the basis for the pure rainwater, were found in 47% and 38% at 1100 Site and Cheju city, respectively. The precipitations in Cheju city were more influenced by the oceanic effect than those in 1100 Site. The acidity contribution was mainly by SO_(4)^(2-) and NO_(3)^(-) in both areas. and the organic acids have contributed to the acidity with only 5-7%. The neutralization factors by NH_(3) were about 46% at both 1100 site and Cheju city, whereas those by CaCO_(3) were 11% and 15% at 1100 site and Cheju city respectively, and the free acidity were both about 35% in average. From the MSA analysis. it was found that the air in Cheju island has been influenced by the pollution from the other areas. The sources of the rainwater components in 1100 Site and Cheju city were also studied with a factor analyzing way and the most probable factors were found to be anthropogenic, oceanic, and soil-sourced. The results of multiple regression analysis have shown that SO_(4)^(??) was dissolved mostly in the form of H_(2)SO_(4), CaSO_(4) and (NH_(4))_(2)SO_(4). and NO_(3)^(-) was in the form of HNO_(3), Ca(NO_(3))_(2) and NH_(4)NO_(3).
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고성능 액체 크로마토그래피에 의한 금속 : Diethyldithiocarbamate Chelates의 용리거동에 관한 연구
강창희,李垣 慶熙大學校 1987 論文集 Vol.16 No.-
The separation behavior of Pb(Ⅱ), Co(Ⅱ), Cu(Ⅱ), Hg(Ⅱ)-diethyldithiocarbamate metal chelates gave been studied by high performance liquid chromatography. The optimum conditions for the separation of four metal-DDTC chelates were determined. As the following conclusions have been obtained. 1. The four metal-DDTC chelates were quantitatively extracted into 2ml of MIBK by shaking for 20-25mins. 2. Metal-DDTC cheltes were successfully separated on Nova-Pak C_18 column (3.9㎜ I. D. x 15㎝, particle size 4μm) using methanol/acetonitrile/water/(40:35:25) as the eluent. And the eluted metal chelates were detected by UV detector (at 254nm). 3. The optimum pH range for the formation of metal-DDTC chelates were from 9.0 to 9.5. 4. The elution-order of the metal-chelates in several extracting solvents is as follows : Pb(Ⅱ)>Co(Ⅱ)>Cu(Ⅱ) Hg(Ⅱ) It was found that these elution-order agreed well the stability of metal-DDTC chelates.
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강창희,김형묵 고려대학교 의과대학 1998 고려대 의대 잡지 Vol.35 No.1
Pentoxifylline is a methylxanthine derivative that has been shown to reduce ischemic injury in several organ systems by improving microvascular circulation. Pentoxifylline has an antineutrophil effect and has been shown to reduce neutrophil-dependent lung injury, and attenuates tumor necrosis factor-induced endothelial permeability. The hemorheologic effect of pentoxifylline is to augment microcirculation by improving deformability and motility of circulating blood cells, lowering blood viscosity, and decreasing neutrophil and platelet aggregation. This study was undertaken to investigate whether pentoxifylline administered during pre-ischemic and post-ischemic periods would ameliorate ischemia-reperfusion injury in preserved isolated rabbit lung. Two groups were evaluated with twelve animals in each group. In the control group, pentoxifylline was not used. In the experimental group, 200mg/L pentoxifylline was added to the 10℃ flushing solution and storage solution (Modified Euro-Collins' solution), in which the lung was immersed for six hours. To the lungs in the experiment group, 20mg/kg bodyweight pentoxifylline was then given through the main pulmonary artery over 5 mins, after which pentoxifylline was continuously infused for the remainder of the reperfusion period at a rate of 0.1mg/kg/min. During pulmonary flush perfusion with modified Euro-Collins' solution mean pulmonary arterial pressures were significantly lower in the pentoxifylline-treated group compared to the control group (p=0.0081). Although the pulmonary arterial pressures were increased in both groups during reperfusion, they were significantly lower in the pentoxifylline-treated group compared to the control group (p=0.0419-0.0002). Pulmonary vascular resistances were significantly lower in the pentoxifylline-treated group than in the control group during reperfusion (p=0.0205-0.0002). Systemic arterial oxygen tensions were decreased in both groups during reperfusion, but were significantly higher in the pentoxifylline-treated group compared to the control group (p<0.0001-<0.0001). Alveolar-arterial oxygen differences were significantly lower in the pentoxifylline-treated group than in the control group during reperfusion (p<0. 0001-<0.0001). Wet/dry weight ratio, tissue water, and water content of the preserved left lung were significantly lower in the pentoxifylline-treated group than in the control group. Microscopically, the intra-alveolar hemorrhage and pulmonary congestion was significantly less in the pentoxifylline-treated group than in the control group. In conclusion, pentoxifylline ameliorated pulmonary ischemia-reperfusion injury as demonstrated by reduction of lung edema and improved gas exchange during the reperfusion period.
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