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Gwak, Woo-Seok,Zhang, Qun,Roy, Animesh The Ichthyological Society of Korea 2019 韓國魚類學會誌 Vol.31 No.4
This study was conducted to know the genetic diversity and population structure of Japanese halfbeak (Hyporhamphus sajori) in the Northeast Asia, using mitochondrial DNA control region. In the present study, a total of 70 individuals were collected from three locations of China (Liaoning), Korea (Tongyeong) and Japan (Wakasa Bay), and 47 individuals sequences from three locations of Japan (Wakasa Bay, Toyama Bay and Mikawa Bay) were downloaded from genbank. A total of 7 haplotypes were identified with 7 polymorphic sites from 358 bp length sequences. Haplotype and nucleotide diversity were very low and ranged from 0 to 0.295±0.156 and 0 to 0.0009±0.0011, respectively. Ancestral haplotype was shared by 94% individuals. An extremely low haplotype and nucleotide diversity, and starlike minimum spanning tree indicated that the species have undergone a recent population expansion after bottleneck. Pairwise F<sub>ST</sub> values were low and there was no significant differences among populations suggesting a gene flow among the populations. Dispersal of the eggs with the aid of drifting seaweed and currents might be the major responsible factor for the genetic homogeneity. 학꽁치(Hyporhamphus sajori)의 유전적 다양성과 집단구조를 조사하기 위해 동북아시아에서 시료를 채집하여 mitochondrial DNA control region (mtDNA CR)을 분석하였다. 시료는 중국(Liaoning), 한국(통영), 일본(Wakasa Bay) 3곳에서 총 70개체를 채집했고, 일본 3곳(Wakasa Bay, Toyama Bay and Mikawa Bay)에서 분석된 47개체의 mtDNA CR 염기서열을 Genbank에서 다운로드했다. 분석결과 총 358 bp가 나타났고, 7개의 변이와 함께 haplotype이 7개 확인되었다. Haplotype diversity과 nucleotide diversity는 각각 0~0.295±0.156 및 0~0.0009±0.0011이고, main haplotype을 94%의 개체가 공유했다. 매우 낮은 haplotype diversity와 nucleotide diversity 그리고 starlike minimum spanning tree는 집단이 최근에 병목현상을 거친 후, 팽창되었음을 나타낸다. 집단 간에 Pairwise F<sub>ST</sub> 값은 낮고 유의하지 않은 것으로 나타났고, 이것은 집단 간 gene flow가 있음을 시사한다. 학꽁치의 genetic homogenity는 부유조와 해류가 주요 원인으로 생각된다.
Gwak Woo-Seok,Tanaka Masaru The Korean Society of Fisheries and Aquatic Scienc 2004 Fisheries and Aquatic Sciences Vol.7 No.4
The effects of temperatures, starvation, and kind of foods on growth, RNA/DNA ratio and protein contents during metamorphosis and early juvenile stage of olive flounder (Paralichthys olivaceus) were examined. During metamorphosis, warm-acclimated fish showed higher RNA and DNA content than those of the cold-acclimated fish, excepting H stage (28 DAH) at which the ratio was higher at cold temperature. RNA/DNA ratio during metamorphosis showed similar values at two temperatures tested. However, after 42 DAH warm-acclimated juveniles had higher DNA content compared with cold-acclimated fish, resulted in marked decreases in RNA/DNA ratios. Higher RNA content at H stage of cold-acclimated fish was consistent with an increase in protein content. Growth of fish rearing at warm temperature was higher than those of fish at cold temperature during all experiments. In starvation experiment, contents of DNA, RNA and protein significantly decreased. Even though there were no significant differences in total length (TL) and body weight between the live mysid-fed and artificial pellet-fed fish at 35 mm TL, both RNA/DNA and protein/DNA ratios of the former group was significantly higher than those of the latter due primarily to lower DNA content of the live mysid-fed group. The results from this study suggest that temperature, starvation and kind of foods should be considered when RNA/DNA ratio applied to assessing the cultured larval and juvenile fish condition.
Analysis of Phenolic compound and isoflavone in various medicinal soybeans
Jae-woo Kim,Eun-young Kang,Su-hyun Seo,Eun-hye Kim,Tae-sic Gwak,Hyun-seok Jeon,Joung-kuk Ahn,Woo-seok Jung,Ill-min Chung 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
Medicinal soybean, which has been proven to be effective in preventing cancer, heart disease, osteoporosis, and renal failure, is drawing people’s attention among other beans in recent days. This experiment was to examine the concentration of phenolic compounds and isoflavone in 53 kinds of soybeans cultivated and collected in Yeo-Ju, Gyeonggi-do region. The total average content of phenol is 29424.34㎍/g and the most and least phenol were detected in No. 35(814.98㎍/g) and No. 28(353.66㎍/g) respectively. Myricetin was detected the most resveratrol whereas detected the least amount which was less than 0.2% of the total amount among all medicinal soybean samples. Total average isoflavone content was 35910.56㎍/g and the most and least isoflavone was detected in No. 15 (996.66 ㎍/g ) and No.9 (476.18㎍/g )respectively . Malonylgenistin was detecteded the most genistein whereas detected the least amount which was less than 0.02 % of the total amount in all medicinal soybean samples. The result of this experiment is considered to be the basic data of the functional food manufacturing by utilizing domestic medicinal soybean.
Review : 9th Indo-Pacific Fish Conference Review
곽우석 ( Woo Seok Gwak ) 한국어류학회 2013 韓國魚類學會誌 Vol.25 No.3
The 9th Indo-Pacific Fish Conference (IPFC9) was held in Okinawa, Japan from 24th to 28th June 2013. Five hundred and thirty two delegates from 36 countries/regions around the world attended. The Conference took place with a welcome address by Dr. Keiichi Matsuura in the Laguna Garden Hotel next to the Okinawa Convention Center in the evening of 23rd June 2013, one day before the official start of the IPFC9. The winners of the two IPFC9 Bleeker Awards, Jeffrey M. Leis (Ecology) and Gerald R. Allen (Systematics) provided excellent talks on “Change in the early lifehistory of Indo-Pacific Fishes” and “The center of Indo-Pacific reef fish diversity”. A total of 462papers were presented, including 328 oral and 134 poster presentations. The main themes of IPFC9included systematics, evolution, zoogeography and phylogeography, biodiversity, ecology, behavior, and conservation. There were 14 symposia, which included topics such as “Top predatory fish in the Indo-Pacific ecosystems”, “Fish diversity across environmental extremes”, “Ontogeny and systematics of Indo-Pacific fishes”, and “Phylogenetics and diversification of the Percomorpha”. IPFC10 will be held in Papeete, Tahiti.