벼 담수직파 논 잡초방제 체계에 따른 올방개 방제 특성

임일빈,임보혁,박재현,임민혁,김대현,장정한,최경진 한국잔디학회 2016 Weed & Turfgrass Science Vol.5 No.4

This study was conducted to investigate the ecology of weed occurrence and to establish an economical paddy field weed control system for direct-seeded rice on water. The main problem weed among annual and perennial weeds was Eleocharis kuroguwai. The control effect of E. kuroguwai was low by the application of azimsulfuron + carfentrazone-ethyl + pyriminobacmethyl at the early stages of rice in directly seeded paddy fields, but most annual weeds including Echinochloa crus-galli were controlled. Also, the additional application of pyrazosulfuron-ethyl + thiobencarb GR at 20 days after first treatment or of bentazone SL at 43 days after the first treatment effectively controlled E. kuroguwai. Tuber formation of E. kuroguwai was inhibited by the additional application of pyrazosulfuron-ethyl + thiobencarb by 84%, but the dormancy rate of old tubers of E. kuroguwai under the soil was higher than that of E. kuroguwai tubers following single application of azimsulfuron + carfentrazone-ethyl + pyriminobac-methyl at the early stages of rice. Tuber formation was inhibited by the additional application of bentazone by approximately 87%, and the old tuber dormancy rate was low in the bentazone treatment. High amounts of old dormant tubers of E. kuroguwai were found in the weedy plots. 벼 담수직파 재배시 제초제 사용에 따른 잡초발생 생태 를 구명하고, 합리적인 잡초방제 체계를 확립하고자 연구한 결과는 다음과 같다.벼 종자 파종 후 12일에 설포닐우레아계 혼합제초제(azimsulfuron + carfentrazone-ethyl + pyriminobac-methyl) 처리는 피 등 일년생잡초의 방제효과는 높았으나, 올방개의 방제효과는 낮았다. 그러나, 설포닐우레아계 혼합제초제 초중기처리 후 20일에 설포닐우레아계 혼합제초제(pyrazosulfuron-ethyl + thiobencarb)의 체계처리는 올방개의방제효과가 높고, 새로운 괴경의 형성량은 적었으나, 구 괴경의 휴면율은 높았다. 또한 설포닐우레아계 혼합제초제의초중기처리와 파종 후 55일에 bentazone 액제의 체계처리는 올방개의 방제효과가 높고, 새로운 괴경의 형성량도 적었으며, 휴면 괴경도 적었다.

Prediction of Seedling Emergence and Early Growth of Eleocharis kuroguwai Ohwi under Evaluated Temperature

Jin-Won Kim(김진원),Byeong Chul Moon(문병철),Soo-Hyun Lim(임수현),Ji-Hoon Chung(정지훈),Do-Soon Kim(김도순) 韓國雜草學會 2010 Weed&Turfgrass Science Vol.30 No.2

기온상승 조건을 부여하기 위해 재식시기를 달리하여 재식 한 올방개의 출아와 초기생장을 평가하고 이들과 유효적산온도간의 관계를 수학적 모델로 해석하기 위한 포트 및 포장평가를 수행하였다. Gompertz 모델을 이용하여 이들의 관계를 비선형회귀로 분석한 결과, 파종일자 및 재식 토양심도에 상관없이 유효적산 온도로 누적출아율 및 초기생장을 양호하게 설명하였다. 올방개의 최대 출아율의 50% 도달에 필요한 유효적산온도는 올방개 괴경 재식심도 1, 3 및 5 cm에서 54.5, 84.0 및 118.0℃이었으며 5엽기에 이르는데 필요한 유효적산온도는 각각 155.3, 188.5 및 215.5℃이었다. 본 연구에서 개발된 모델식을 이용하여 계산한 결과 평균기온이 2℃ 상승한 조건에서 올방개의 50 % 출아는 심도에 따라 약 1 - 2일 빨라지고, 5엽기에 도달하는 날짜도 약 2 - 3일 빨라질 것으로 예측되었다. 따라서 2℃ 기온상승 조건에서 올방개를 효과적으로 방제하기 위해서는 현재의 제초제 처리시기보다 약 2 - 3일 빨라져야 할 것으로 판단된다. Field and pot experiments were conducted to investigate seedling emergence and early growth of Eleocharis kuroguwai panted on different dates. Non-linear regression analyses of observed data against effective accumulated temperature (EAT) with the Gompertz model showed that the Gompertz model works well in describing seedling emergence and early growth of E. kuroguwai regardless of planting date and soil burial depth. EATs required for 50% of the maximum seedling emergence of E. kuroguwai planted at 1, 3 and 5 cm soil burial depth in the pot experiment were estimated to be 54.5, 84.0 and 118.0℃, respectively, and 56.7℃ when planted at 1 cm in the field experiment. EATs required for 50% of the maximum leaf number of E. kuroguwai planted at 1, 3 and 5 cm soil burial depth in the pot experiment were estimated to be 213.3, 249.0 and 291.6℃, respectively, and 239.5℃ when planted at 1 cm in the field experiment. Therefore, models developed in this study thus predicted that if rotary tillage with water is made on 27 May under +2℃ elevated temperature condition, dates for 50% of the maximum seedling emergence, 5 leaf stage and 5 cm plant height of E. kuroguwai buried at 3 cm soil depth were predicted to be 2 June, 10 June and 12 June. These dates are 1 day earlier for the seedling emergence and 3 days earlier for the early growth as compared with current temperature condition, suggesting that earlier application of herbicides is required for effective control of E. kuroguwai.

Selectivity of Tefuryltrione between Rice and Eleocharis kuroguwai

송종석,Yong Seog Park,박민원,Jeong Deug Lee,김도순 한국잔디학회 2016 Weed & Turfgrass Science Vol.5 No.4

Tefuryltrione is a new hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor, which has been recently registered for the use for paddy rice, Korea. Dose-response studies were conducted to compare rice safety and weed control efficacy of tefuryltrione against Eleocharis kuroguwai. When rice and E. kuroguwai were applied at a range of doses of tefuryltrione, GR90 values (the dose required to inhibit weed growth by 90%) of E. kuroguwai were 82.38-93.39 g a.i. ha−1 in two independent experiments. The GR10 values (the dose required to inhibit rice growth by 10%) of tefuryltrione for rice were 297.77-471.54 g a.i.ha−1. As a result, the selectivity indices (GR10 for rice/GR90 for E. kuroguwai) of tefuryltrione were 3.19-5.72. Therefore, these results demonstrate that tefuryltrione has a relatively high selectivity between rice and E. kuroguwai with a high herbicidal activity against E. kuroguwai and a good rice safety.

Selectivity of Tefuryltrione between Rice and Eleocharis kuroguwai

Song, Jong-Seok,Park, Yong Seog,Park, Min-Won,Lee, Jeong Deug,Kim, Do-Soon The Korean Society of Weed ScienceThe Turfgrass So 2016 Weed & Turfgrass Science Vol.5 No.4

Tefuryltrione is a new hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor, which has been recently registered for the use for paddy rice, Korea. Dose-response studies were conducted to compare rice safety and weed control efficacy of tefuryltrione against Eleocharis kuroguwai. When rice and E. kuroguwai were applied at a range of doses of tefuryltrione, $GR_{90}$ values (the dose required to inhibit weed growth by 90%) of E. kuroguwai were $82.38-93.39g\;a.i.\;ha^{-1}$ in two independent experiments. The $GR_{10}$ values (the dose required to inhibit rice growth by 10%) of tefuryltrione for rice were $297.77-471.54g\;a.i.\;ha^{-1}$. As a result, the selectivity indices ($GR_{10}$ for $rice/GR_{90}$ for E. kuroguwai) of tefuryltrione were 3.19-5.72. Therefore, these results demonstrate that tefuryltrione has a relatively high selectivity between rice and E. kuroguwai with a high herbicidal activity against E. kuroguwai and a good rice safety.

Modelling Rice Competition with Echinochloa crus-galli and Eleocharis kuroguwai in Transplanted Rice Cultivation

Moon, Byeong-Chul,Cho, Seung-Hyun,Kwon, Oh-Do,Lee, Sun-Gye,Lee, Byun-Woo,Kim, Do-Soon 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.2

Field experiments were conducted to investigate rice - Echinochloa crus-galli and rice - Eleocharis kuroguwai competition under transplanted rice cultivation in four major rice production areas; Suwon, Daejeon, Iksan, and Naju in Korea. Rice yield data were used to predict rice yield as a function of plant densities of E. crus-galli and E. kuroguwai using a rectangular hyperbola and to determine economic threshold (ET) levels of the weeds. Both weed species significantly reduced number of tillers at early rice growth stage, resulting in significant reduction in number of spikes, and the other yield components such as number of grains, maturity and 1,000-grain weight at later growth stage. The weed competitivity represented by parameter ranged from 0.0145 to 0.0346 for E. crus-galli and from 0.0037 to 0.0187 for E. kuroguwai, indicating that the competition effect of E. crus-galli on rice yield was slightly greater than that of E. kuroguwai. The ET values of E. crus-galli were between 0.298 and 1.078 plants $m^{-2}$, while those of E. kuroguwai were between 0.848 and 5.298 plants $m^{-2}$, depending on weed competitivity and herbicide price. Therefore, our results can be used to support decision-making on herbicide application for E. crus-galli and E. kuroguwai management in transplanted rice cultivation.

Modelling Rice Competition with Echinochloa crus-galli and Eleocharis kuroguwai in Transplanted Rice Cultiva

문병철,이순계,이변우,김도순,조성현,권오도 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.2

Field experiments were conducted to investigate rice - Echinochloa crus-galli and rice - Eleocharis kuroguwai competition under transplanted rice cultivation in four major rice production areas; Suwon, Daejeon, Iksan, and Naju in Korea. Rice yield data were used to predict rice yield as a function of plant densities of E. crus-galli and E. kuroguwai using a rectangular hyperbola and to determine economic threshold (ET) levels of the weeds. Both weed species significantly reduced number of tillers at early rice growth stage, resulting in significant reduction in number of spikes, and the other yield components such as number of grains, maturity and 1,000-grain weight at later growth stage. The weed competitivity represented by parameter βranged from 0.0145 to 0.0346 for E. crus-galli and from 0.0037 to 0.0187 for E. kuroguwai, indicating that the competition effect of E. crus-galli on rice yield was slightly greater than that of E. kuroguwai. The ET values of E. crus-galli were between 0.298 and 1.078 plants m-², while those of E. kuroguwai were between 0.848 and 5.298 plants m-², depending on weed competitivity and herbicide price. Therefore, our results can be used to support decision-making on herbicide application for E. crus-galli and E. kuroguwai management in transplanted rice cultivation.

Factors Affecting Sporulation of a Mycoherbicide, Epicoccosorus nematosporus, on the Lesion of Eleocharis kuroguwai

Hong, Yeon-Kyu,Hyun, Jong-Nae,Cho, Jae-Min,Uhm, Jae-Youl,Kim, Soon-Chul The Korean Society of Plant Pathology 2002 Plant Pathology Journal Vol.18 No.2

Effects of temperature and dew period on sporulation of a mycoherbicide, Epicoccosorus nematosporus, on the lesion of its host, Eleocharis kuroguwai were determined. Conidia formation was first observed after 10 days on plants incubated for either 12 or 16 h in a dew chamber at 28$^{\circ}C$; 16 h dew period resulted in more conidia formation. As the dew period was decreased to less than 8 h, fewer conidia formed. Conidial production was most abundant at 28$^{\circ}C$ and produced as much as 3.3$\times$10$^4$conidia per lesion, while 0.1$\times$10$^3$and 2.3$\times$10$^3$conidia per lesion were produced at 16$^{\circ}C$ and 36$^{\circ}C$, respectively. Alternating temperature regimes, i.e., 30/15, 30/20, 28/20, and 28/15$^{\circ}C$ (day/night) were much better than constant temperature, i.e., 30/30, 28/28/, and 20/2$0^{\circ}C$ for sporulation. In the second sporulation, there were as much as 3.1$\times$10$^4$conidia per lesion (ca. <50% of the first sporulation). Then, sporulation dropped sharply to 6.2$\times$10$^2$conidia per lesion in the third sporulation. Results of this study suggest that temperature combined with dew period is the primary limiting factor in the use of E. nematosporus as a mycoherbicide off, kuroguwai.

Factors Affecting Sporulation, Germination, and Appressoria Formation of Epicoccosorus nematosporus as a Mycoherbicide Under Controlled Environments

홍연규,엄재열,이봉춘,조재민,김순철 한국식물병리학회 2002 Plant Pathology Journal Vol.18 No.1

Effects of temperature and dew period on sporulation of a mycoherbicide, Epicoccosorus nematosporus, on the lesion of its host, Eleocharis kuroguwai were determined. Conidia formation was first observed after 10 days on plants incubated for either 12 or 16 h in a dew chamber at 28oC; 16 h dew period resulted in more conidia formation. As the dew period was decreased to less than 8 h, fewer conidia formed. Conidial production was most abundant at 28oC and produced as much as 3.3´104 conidia per lesion, while 0.1´103 and 2.3´103 conidia per lesion were produced at 16oC and 36oC, respectively. Alternating temperature regimes, i.e., 30/ 15, 30/20, 28/20, and 28/15oC (day/night) were much better than constant temperature, i.e., 30/30, 28/28/, and 20/20oC for sporulation. In the second sporulation, there were as much as 3.1´104 conidia per lesion (ca. <50% of the first sporulation). Then, sporulation dropped sharply to 6.2´102 conidia per lesion in the third sporulation. Results of this study suggest that temperature combined with dew period is the primary limiting factor in the use of E. nematosporus as a mycoherbicide of E. kuroguwai.

Evaluation of Control Efficacy of Biocontrol Agent, Epicoccosorus nematosporus on Eleocharis kuroguwai in the Field

Hong, Yeon-Kyu,Cho, Jae-Min,Uhm, Jae-Youl,Lee, Bong-Choon,Hyun, Jong-Nae,Hwang, Jae-Bok,Kim, Soon-Chul The Korean Society of Plant Pathology 2003 Plant Pathology Journal Vol.19 No.2

This study was conducted to determine the efficacy of Epicoccosorus nematosporus for the control of Eleocharis kuroguwai and to evaluate the meteorological factors which affect weeding efficacy in field conditions for three years (1996-1998). The best time to control E. kuroguwai with E. nematosporus as a biological control agent in the field was in July, when temperature ranged from 20.4 to $23.4^{\circ}C$; the surface wetness duration was 12.6-16.1 hours, and application time of 6:00 p.m. and 8:00 p.m.; and weeding efficacy was 81-90%. On 10 June 1996 in Milyang area, where the field experiments were performed, mean temperature was $16.5^{\circ}C$ with 11.3 hours of dew duration. Meanwhile, on 20 Aug. 1996 the temperature was $21.3^{\circ}C$ with 15.4 hours of dew duration. During these periods, the weeding efficacy was recorded at 61.8 and 60.8%, respectively. Time required for complete plant death was 25.8 and 25.6 days at application times 10 June and 20 Aug., respectively. At the time of application on 7,18, and 27 July 1996, mean temperature was 20.4-$23.4^{\circ}C$ with 12.6-16.5 hours of dew duration. The weeding efficacies of these periods were very hi일 with 81.4-90.8%. Three years of field observations from 1996 to 1998 showed that infection in the field can occur at any time through the summer season, although total infection rates vary between months and between years. In 1996, plant infection rapidly increased from 56% on 30 June, 82.4% on 15 July, 94.6% on 15 August, and 92.8% on 15 September under favorable meteorological conditions such as minimum temperature of $17.6^{\circ}C$ and maximum temperature of $27.1^{\circ}C$, with 86% relative humidity and 977.5 mm of rainfall during E. kuroguwai growing season. However, in 1997, the disease incidence was very low because of unfavorable weather conditions brought about by the hot temperature and the low amount of rainfall at 321.5 mm. Disease progress was slow from 24.4% on 30 June to 49.2% at the end of the growing season.

Eleocharis kuroguwai Ohwi Ameliorates LPS-mediated Inflammation by Suppressing MAPKs Signaling

Kang-Hoon Kim(김강훈),Eun Young Bae(배은영),Hyun Goo Lim(임현구),Mi Heui Kim(김미희),Tae-Seok Oh(오태석),Seung Woong Lee(이승웅) 한국생명과학회 2020 생명과학회지 Vol.30 No.5

벼 농사에서 마릅으로 불리는 올방개는 문제의 잡초 종류 중 하나로서 제초 작용의 대상이다. 하지만, 올방개는 임상적으로 황달, 해열 및 통경을 위한 전통 의학에 쓰여왔다. 올방개는 오랫동안 임상적으로 사용되어왔음에도 불구하고, 분자생물학 기반의 생물학적 효과는 아직 밝혀지지 않았다. 때문에, 본 연구진은 LPS로 유도된 대식세포 내에서 올방개 추출물 처리에 의한 NO 생산량을 스크리닝 함으로써 항염증 효과를 조사했다. 가장 효과적인 항염증 fraction을 찾기 위해, 본 연구진은 HP20 column chromatography를 이용하여 5 종류의 EtOH sub-fraction을 동정하였다; 20%, 40%, 60%, 80%, 및 100% EtOH. 동정한 sub-fraction 중에서, 60%와 80% 성분에서 가장 유의성 있는 NO 생산의 저해를 나타냈으며, 세포독성 효과도 나타내지 않는 결과를 나타냈다. 추가적으로 80% sub-fraction은 유의성 있는 iNOS 저해와 전염증성 매개체의 mRNA; IL-6, TNF-α, 그리고 IL-1β를 저해하였으며, 이 결과는 MAPKs 세포신호전달과 관련 있는 JNK 및 ERK 단백질의 인산화 저해가 원인임을 밝혔다. 본 연구진의 결과는 올방개 추출물 80% sub-fraction EtOH가 항염증 활성을 가장 효과적으로 저해하는 성분 임을 밝혔으며, 이는 MAPKs 세포신호전달의 과 발현에 의한 염증성 질병의 치료제 후보 성분 일 수 있음을 시사한다. In rice agriculture, Eleocharis kuroguwai Ohwi (olbanggae) is a target for herbicidal intervention as a problem weed although it has also long been used clinically as a traditional medicine for jaundice, fever, and blood flow. E. kuroguwai has been evaluated in many clinical trials, but its molecular biological advantages are still unknown. Here, we investigate the anti-inflammatory effects of E. kuroguwai 80% ethanol extracts by screening NO production in LPS-induced macrophage activation. To find the most effective fractions, we partitioned five sub-fractions using HP20 column chromatography, namely 20%, 40%, 60%, 80%, and 100%. Of these, the 60% and 80% sub-fractions were found to significantly inhibit NO production; there were no toxicological effects at any concentration. In addition, the 80% sub-fraction inhibited significantly the iNOS and the mRNA of the pro-inflammatory mediators IL-6, TNF-α, and IL-1β by inhibiting the phosphorylation of JNK and ERK pathways associated with MAPKs signaling. Our results suggest that the 80% E. kuroguwai sub-fraction has the most significant anti-inflammatory effects of inhibiting iNOS and pro-inflammatory mediators and suppressing the phosphorylation of JNK and ERK. Therefore, the 80% sub-fraction of E. kuroguwai extract may be a therapeutic candidate for inflammatory diseases associated with the overexpression of MAPKs.