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이경호,권진호,김동명 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
Cell-free protein synthesis (CFPS) is a powerful tool for rapid production of recombinant proteins as well as for functional analysis of genes.The unique flexibility and throughput of cell-free protein synthesis have made this technology a promising alternative to conventional cell-based protein production methods. Despite the versatility of CFPS,however, it requires further improvements in protein productivity. One of the measures for improving yield is a continuous-exchange cell-free (CECF) system. The CECF system supports extended syntheses of target proteins through continuous supply and removal of substrate materials and by-products, respectively. However, previous CECF platforms have suffered from the complexity of reaction devices and unacceptably high reagent costs. In this study, we tried to simplify CECF system with cost-effective substrates. First, we designed a simplified CECF device using common lab materials. In addition, we prepared a concentrated master mix solution to simplify the reaction schemes. Finally, nucleoside triphosphates (NTP) were replaced with nucleoside monophosphates(NMP) that are far more cost-effective. These simplified CECF platform with cost-effective substrates led to a significant increase yield of protein synthesis with a fraction of the cost required for conventional CECF reactions.
미세유체반응기를 이용한 core/shell 연속 합성 시스템을 이용한 CdSe/ZnS 양자점 합성 및 분석
홍명환,주소영,강이승,이찬기,Hong, Myung Hwan,Joo, So Young,Kang, Lee-Seung,Lee, Chan Gi 한국분말야금학회 2018 한국분말재료학회지 (KPMI) Vol.25 No.2
Core/shell CdSe/ZnS quantum dots (QDs) are synthesized by a microfluidic reactor-assisted continuous reactor system. Photoluminescence and absorbance of synthesized CdSe/ZnS core/shell QDs are investigated by fluorescence spectrophotometry and online UV-Vis spectrometry. Three reaction conditions, namely; the shell coating reaction temperature, the shell coating reaction time, and the ZnS/CdSe precursor volume ratio, are combined in the synthesis process. The quantum yield of the synthesized CdSe QDs is determined for each condition. CdSe/ZnS QDs with a higher quantum yield are obtained compared to the discontinuous microfluidic reactor synthesis system. The maximum quantum efficiency is 98.3% when the reaction temperature, reaction time, and ZnS/CdSe ratio are $270^{\circ}C$, 10 s, and 0.05, respectively. Obtained results indicate that a continuous synthesis of the Core/shell CdSe/ZnS QDs with a high quantum efficiency could be achieved by isolating the reaction from the external environment.
Neman Izadi,Banafsheh Haji Ali,Mohammad Sajjad Shahin,Majid Baghdadi 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.8
Recently, the in-situ removal of contaminants by iron nanoparticles has been considered due to their nontoxicity,abundance, ease of production, and cost-effectiveness, which can be accomplished by injecting them underground. In this study, nZVI was synthesized using a novel continuous synthesis method using flaxseed glaze as a green,non-toxic, and low-priced coating. The produced nanoparticles were characterized by dynamic diffraction analysis(DLS), field electron microscopy (FE-SEM), X-ray spectroscopy (EDX), and infrared spectroscopy (FTIR) spectroscopy. Batch experiments were conducted to evaluate the effect of Cr(VI) concentration, FG-nZVI dosage, pH, and coexistingcomponents (Total dissolved solids, Humic acid, and NO3) on Cr(VI) removal. Results of the characterization andidentification, and stability tests indicated that nanoparticles synthesized by using this continuous synthesis system weresmaller and more regular shaped than those prepared by conventional synthesis. According to the results, the Cr(VI)residual level increased by increasing the initial Cr(VI) concentration and decreased by increasing the nZVI coatedwith flaxseed glaze (FG-nZVI) dosage. At the Cr(VI) initial concentration of 4mg L1, the Cr(VI) was removed entirelyat almost all dosages of FG-nZVI. Optimal amount of FG-nZVI was 62.73mg L1 when applied at 4mg L1 of Cr(VI)at optimum pH 6.64, resulting in the Cr(VI) residual concentration of 0.05mg L1. The results of saturated porousmedia showed that injection background solutions enhanced the transfer of nanoparticles in the porous medium, resultingin the adequate removal along the desired radius. The results illustrated that using FG-nZVI can be effective forpractical groundwater remediation.
고서영,이경호,김동명 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.6
The open nature of cell-free synthesis allows customization of the reaction conditions for given target molecules using diverse biological and non-biological substances. This study demonstrates that non-ionic detergents can be used to enhance translation during the synthesis of peptides in a cell-free system derived from an Escherichia coli extract. The yield of the antimicrobial peptide Cecropin P1 was markedly increased in the presence of detergents. The stimulatory effect of detergents was not limited to the Cecropin P1 peptide, but the detergent also enhanced the translation of other antimicrobial peptides. Furthermore, the enhanced translation rate by detergents was maintained for extended periods by a continuous exchange cell-free synthesis reaction, leading to production of antimicrobial peptides with markedly improved yields.