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Sarkar, P.,Kumar, H.,Rawat, M.,Varshney, V.P.,Goswami, T.K.,Yadav, M.C.,Srivastava, S.K. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.7
The efficacy of garlic extract and $PGF_2{\alpha}$ in the treatment of endometritis in cows was evaluated. A total of 26 parous cows affected with endometritis were randomly allocated into three groups. Group I (n = 10) animals were infused (IU) with 10 ml garlic extract mixed with 90 ml normal saline, three times at 12 h interval starting from the day of estrum, whereas the animals of Group II (n = 10) were treated with a single injection of $PGF_2{\alpha}$ (25 mg Lutalyse) on the $10^{th}$ to $12^{th}$ day after estrus, and group III (n = 6) remained as control. Cervico-vaginal mucus (CVM) was collected from each animal at pre- and post-treatment estrus and subjected to white side test, pH determination and total bacterial load. The clinical recovery of cows was assessed by negative white side test reaction, pH value and total bacterial count of CVM at subsequent estrus. The recovered animals were inseminated with frozen-thawed semen twice at 12 h intervals and pregnancy was confirmed at 45-60 days following insemination. A significant decline (p<0.05) in pH of CVM was observed in both the treatment groups at subsequent estrus. After treatment there was a significant (p<0.05) reduction in bacterial load, whereas, it was increased in control group. A total number of 65 isolates were identified in CVM samples comprising mostly of facultative anaerobic bacteria. Plasma $T_4$ and $T_3$ concentrations were increased in all the treated animals, whereas, a decline was observed in cortisol levels following treatment. The overall conception rate was 50% in treated groups as compared to nil pregnancy in the control.
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Yasunori Kawagoshi,Masaharu Nakao,Naoe Hino,Tomonori Iwasa,Kenji Furukawa 한국습지학회 2007 한국습지학회지 Vol.9 No.1
Effect of nitrogen-load condition on hydrogen (H2) production and bacterial community in a continuous anaerobic hydrogen fermentation were investigated. The slight H2 production on extremely low nitrogen-load condition (C/N ratio: 180) at the start-up period. The highest H2 production was obtained when the C/N ratio was 36, the H2 production yield (mol-H2 / mol-glucose) reached to 1.7, and it was indicated that Clostridium pasteurianum mainly contributed to the H2 production. The H2 production was decreased on both the lower (C/N: 72) and higher (C/N: 18) nitrogen-load conditions. The excess nitrogen-load was not always suitable for the hydrogen production. The fluctuation of H2 production seemed to be caused by a change in the bacterial community according to the nitrogen-load condition, while a recovery of H2 productivity was possible by a control of nitrogen-load condition through the bacterial community change. When the nitrogen-load condition was not suitable for hydrogen production, the lactic acid concentration was increased and also lactic acid bacteria were definitely detected, which suggested that the competition between hydrogen fermentator and lactic acid producer was occurred. These results demonstrated that the nitrogen-load condition affect on the H2 productivity through the change of bacterial community in anaerobic hydrogen fermentation.
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Kawagoshi, Yasunori,Nakao, Masaharu,Hino, Naoe,Iwasa, Tomonori,Furukawa, Kenji Korean Wetlands Society 2007 한국습지학회지 Vol.9 No.1
Effect of nitrogen-load condition on hydrogen ($H_2$) production and bacterial community in a continuous anaerobic hydrogen fermentation were investigated. The slight $H_2$ production on extremely low nitrogen-load condition (C/N ratio: 180) at the start-up period. The highest $H_2$ production was obtained when the C/N ratio was 36, the $H_2$ production yield ($mol-H_2/mol-glucose$) reached to 1.7, and it was indicated that Clostridium pasteurianum mainly contributed to the $H_2$ production. The $H_2$ production was decreased on both the lower (C/N: 72) and higher (C/N: 18) nitrogen-load conditions. The excess nitrogen-load was not always suitable for the hydrogen production. The fluctuation of $H_2$ production seemed to be caused by a change in the bacterial community according to the nitrogen-load condition, while a recovery of $H_2$ productivity was possible by a control of nitrogen-load condition through the bacterial community change. When the nitrogen-load condition was not suitable for hydrogen production, the lactic acid concentration was increased and also lactic acid bacteria were definitely detected, which suggested that the competition between hydrogen fermentator and lactic acid producer was occurred. These results demonstrated that the nitrogen-load condition affect on the $H_2$ productivity through the change of bacterial community in anaerobic hydrogen fermentation.
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이지윤,김경화,박신영,윤성영,김곤호,이용무,류인철,설양조 대한치주과학회 2019 Journal of Periodontal & Implant Science Vol.49 No.5
Purpose: Direct application of atmospheric-pressure plasma jets (APPJs) has been established as an effective method of microbial decontamination. This study aimed to investigate the bactericidal effect of direct application of an APPJ using helium gas (He-APPJ) on Porphyromonas gingivalis biofilms on sandblasted and acid-etched (SLA) titanium discs. Methods: On the SLA discs covered by P. gingivalis biofilms, an APPJ with helium (He) as a discharge gas was applied at 3 different time intervals (0, 3, and 5 minutes). To evaluate the effect of the plasma itself, the He gas–only group was used as the control group. The bactericidal effect of the He-APPJ was determined by the number of colony-forming units. Bacterial viability was observed by confocal laser scanning microscopy (CLSM), and bacterial morphology was examined by scanning electron microscopy (SEM). Results: As the plasma treatment time increased, the amount of P. gingivalis decreased, and the difference was statistically significant. In the SEM images, compared to the control group, the bacterial biofilm structure on SLA discs treated by the He-APPJ for more than 3 minutes was destroyed. In addition, the CLSM images showed consistent results. Even in sites distant from the area of direct He-APPJ exposure, decontamination effects were observed in both SEM and CLSM images. Conclusions: He-APPJ application was effective in removing P. gingivalis biofilm on SLA titanium discs in an in vitro experiment.
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Anbarasi, S.,Muralidharan, S. The Korean Institute of Electrical Engineers 2017 Journal of Electrical Engineering & Technology Vol.12 No.3
Penetration of renewable energy sources makes the modern interconnected power systems to have more intelligence and flexibility in the control. Hence, it is essential to maintain the system frequency and tie-line power exchange at nominal values using Load Frequency Control (LFC) for efficient, economic and reliable operation of power systems. In this paper, intelligent tuning of the Proportional Integral Derivative (PID) controller for LFC in an interconnected power system is considered as a main objective. The chosen problem is formulated as an optimization problem and the optimal gain parameters of PID controllers are computed with three innovative swarm intelligent algorithms named Particle Swarm Optimization (PSO), Bacterial Foraging Optimization Algorithm (BFOA) and hybrid Bacterial Foraging Particle Swarm Optimization (BFPSO) and a comparative study is made between them. A new objective function designed with necessary time domain specifications using weighted sum approach is also offered in this report and compared with conventional objective functions. All the simulation results clearly reveal that, the hybrid BFPSO tuned PID controller with proposed objective function has better control performances over other optimization methodologies.
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S.Anbarasi,S. Muralidharan 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.3
Penetration of renewable energy sources makes the modern interconnected power systems to have more intelligence and flexibility in the control. Hence, it is essential to maintain the system frequency and tie-line power exchange at nominal values using Load Frequency Control (LFC) for efficient, economic and reliable operation of power systems. In this paper, intelligent tuning of the Proportional Integral Derivative (PID) controller for LFC in an interconnected power system is considered as a main objective. The chosen problem is formulated as an optimization problem and the optimal gain parameters of PID controllers are computed with three innovative swarm intelligent algorithms named Particle Swarm Optimization (PSO), Bacterial Foraging Optimization Algorithm (BFOA) and hybrid Bacterial Foraging Particle Swarm Optimization (BFPSO) and a comparative study is made between them. A new objective function designed with necessary time domain specifications using weighted sum approach is also offered in this report and compared with conventional objective functions. All the simulation results clearly reveal that, the hybrid BFPSO tuned PID controller with proposed objective function has better control performances over other optimization methodologies.
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Lee, Ji-Yoon,Kim, Kyoung-Hwa,Park, Shin-Young,Yoon, Sung-Young,Kim, Gon-Ho,Lee, Yong-Moo,Rhyu, In-Chul,Seol, Yang-Jo Korean Academy of Periodontology 2019 Journal of Periodontal & Implant Science Vol.49 No.5
Purpose: Direct application of atmospheric-pressure plasma jets (APPJs) has been established as an effective method of microbial decontamination. This study aimed to investigate the bactericidal effect of direct application of an APPJ using helium gas (He-APPJ) on Porphyromonas gingivalis biofilms on sandblasted and acid-etched (SLA) titanium discs. Methods: On the SLA discs covered by P. gingivalis biofilms, an APPJ with helium (He) as a discharge gas was applied at 3 different time intervals (0, 3, and 5 minutes). To evaluate the effect of the plasma itself, the He gas-only group was used as the control group. The bactericidal effect of the He-APPJ was determined by the number of colony-forming units. Bacterial viability was observed by confocal laser scanning microscopy (CLSM), and bacterial morphology was examined by scanning electron microscopy (SEM). Results: As the plasma treatment time increased, the amount of P. gingivalis decreased, and the difference was statistically significant. In the SEM images, compared to the control group, the bacterial biofilm structure on SLA discs treated by the He-APPJ for more than 3 minutes was destroyed. In addition, the CLSM images showed consistent results. Even in sites distant from the area of direct He-APPJ exposure, decontamination effects were observed in both SEM and CLSM images. Conclusions: He-APPJ application was effective in removing P. gingivalis biofilm on SLA titanium discs in an in vitro experiment.
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Mikhail I. Kogan,Khalid S. Ibishev,Yulia L. Naboka,Irina A. Gudima,Akhmed Kh. Ferzauli,Ruslan S. Ismailov,Kurt G. Naber 대한비뇨의학회 2022 Investigative and Clinical Urology Vol.63 No.3
Purpose: Because of the insufficient efficacy of the current treatment of chronic bacterial prostatitis (CBP), it is justified to search for a more effective antibiotic therapy (ABT). Materials and Methods: This single-centre prospective observational comparative study was conducted in 2012 to 2019 (patients: 60 men with CBP; age: 20–45 y). The clinical examination was performed on admission and at 1, 3, 6, or 12 months. All patients underwent the Meares–Stamey test to obtain expressed prostatic secretion (EPS) and/or post-massage urine (PMU) samples for extended bacteriological examination. The patients were randomly divided into 2 treatment groups (30/30 patients): group I, fluoroquinolones (FQs); group II, a combination of FQs with cephalosporins/macrolides with a treatment duration of 1 month. Results: Patients of both groups had severe symptomatic CBP with an average duration of 4 years. Twenty-three microorganisms (15 aerobes, 9 anaerobes) were identified in PMU. At 3 months follow-up, a positive clinical effect was noted in both groups, which was significant (p<0.05) only in group II concerning NIH-CPSI questionnaire, leukocyturia, prostate volume, maximum urine flow, and decreased pathospermia. At 6 months follow-up, in group II the frequency of Escherichia coli and Enterococcus spp. decreased significantly. In group I aerobes changed only insignificantly from the initial level, but anaerobes increased significantly. In group II the titers of both, aerobes and anaerobes, were significantly lower (p<0.05) at 6 months follow-up as compared to initial values. Conclusions: ABT targeting all taxa in EPS/PMU is a more effective alternative to standard therapeutic regimens for CBP.
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Bekara, Farid,Vitse, Julian,Fluieraru, Sergiu,Masson, Raphael,De Runz, Antoine,Georgescu, Vera,Bressy, Guillaume,Labbe, Jean Louis,Chaput, Benoit,Herlin, Christian Korean Society of Plastic and Reconstructive Surge 2018 Archives of Plastic Surgery Vol.45 No.2
Debridement is a crucial component of wound management. Recent technologies such as hydrosurgery (Versajet), ultrasound therapy (the MIST therapy device), or plasma-mediated bipolar radio-frequency ablation therapy (Coblation) seem to represent interesting alternatives for wound debridement. The purpose of this systematic review was to describe, evaluate, and compare these three recently developed methods for the management of chronic wounds. In January 2016, an electronic database search was conducted of MEDLINE, PubMed Central, and Embase for articles concerning these three innovative methods for the management of chronic wounds. A total of 389 references were identified by our search strategy, and 15 articles were included. We extracted data regarding the number and age of patients, indications, operating time, number of procedures, costs, wound healing time, decrease in exudation, perioperative blood loss, bacterial load, and the occurrence of complications. The 15 articles included studies that involved 563 patients who underwent hydrosurgery (7 studies), ultrasound therapy (6 studies), or Coblation (2 studies). Six randomized controlled trials were included that compared the use of a scalpel or curette to hydrosurgery (2 studies) or ultrasound therapy (6 studies). Hydrosurgery, in addition to being a very precise and selective tool, allows significantly faster debridement. Ultrasound therapy provides a significant reduction of exudation, and improves the wound healing time. No comparative study dedicated to Coblation was identified. Despite the obvious clinical interest of the topic, our review of the current literature revealed a lack of prospective randomized studies comparing these devices with each other or with standard techniques, particularly for Coblation and hydrosurgery.
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Farid Bekara,Julian Vitse,Sergiu Fluieraru,Raphael Masson,Antoine De Runz,Vera Georgescu,Guillaume Bressy,Jean Louis Labbé,Benoit Chaput,Christian Herlin 대한성형외과학회 2018 Archives of Plastic Surgery Vol.45 No.2
Debridement is a crucial component of wound management. Recent technologies such as hydrosurgery (Versajet), ultrasound therapy (the MIST therapy device), or plasma-mediated bipolar radio-frequency ablation therapy (Coblation) seem to represent interesting alternatives for wound debridement. The purpose of this systematic review was to describe, evaluate, and compare these three recently developed methods for the management of chronic wounds. In January 2016, an electronic database search was conducted of MEDLINE, PubMed Central, and Embase for articles concerning these three innovative methods for the management of chronic wounds. A total of 389 references were identified by our search strategy, and 15 articles were included. We extracted data regarding the number and age of patients, indications, operating time, number of procedures, costs, wound healing time, decrease in exudation, perioperative blood loss, bacterial load, and the occurrence of complications. The 15 articles included studies that involved 563 patients who underwent hydrosurgery (7 studies), ultrasound therapy (6 studies), or Coblation (2 studies). Six randomized controlled trials were included that compared the use of a scalpel or curette to hydrosurgery (2 studies) or ultrasound therapy (6 studies). Hydrosurgery, in addition to being a very precise and selective tool, allows significantly faster debridement. Ultrasound therapy provides a significant reduction of exudation, and improves the wound healing time. No comparative study dedicated to Coblation was identified. Despite the obvious clinical interest of the topic, our review of the current literature revealed a lack of prospective randomized studies comparing these devices with each other or with standard techniques, particularly for Coblation and hydrosurgery.
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