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Xiao-Li Wang,Xing Fan,Jian Zeng,Li-Na Sha,Hai-Qin Zhang,Hou-Yang Kang,Rui-Wu Yang,Li Zhang,Chun-Bang Ding,Yong-Hong Zhou 한국유전학회 2012 Genes & Genomics Vol.34 No.3
To estimate the phylogeny and molecular evolution of a single-copy nuclear disrupted meiotic cDNA (DMC1) gene within the StH genome species, two DMC1 homoeologous sequences were isolated from nearly all the sampled StH genome species and were analyzed with those from seven diploid taxa representing the St and H genomes in Triticeae. Sequence diversity patterns and genealogical analysis suggested that (1) there is a close relationship among North American StH genome species;(2) the DMC1 gene sequences of the StH genome species from North America and Eurasia are evolutionarily distinct;(3) the StH genome polyploids have higher levels of sequence diversity in the St genome homoeolog than the H genome homoeolog;(4) the DMC1 sequence may evolve faster in the polyploid species than in the diploids; (5) high dN and dN/dS values in the St genome within polyploid species could be caused by low selective constraints or AT-biased mutation pressure. Our result provides some insight on evolutionary dynamics of duplicate DMC1 gene, the polyploidization events and phylogeny of the StH genome species.
Zohreh Abdollahi,Masoud Frounchi,Susan Dadbin 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.3
High molecular weight homopolymers of acrylamide (PAM) and (2-methacryloyloxyethyl) trimethyl ammonium chloride (PDMC) and copolymer ofDMC with acrylamide (P(AM-co-DMC)) were synthesized using solution polymerization technique. The synthesized polymers were characterized by Fourier transform infrared (FTIR), 1HNMR, 13CNMR spectroscopy and differential scanning calorimetry (DSC)and viscometery. 1HNMR and 13CNMR studies confirmed the structure of the synthesized polymers. The behavior of the synthesized polymers was investigated in water–acetone and water–methanolmixtures. The plot of intrinsic viscosity of either PAM or P(AM-co-DMC) with respect to acetone (methanol)/water ratio in water–acetone (methanol) demonstrated a maximum indicating a balance between acetone or methanol hydrogen bonds with water and non-solvency effect of acetone or methanol. However, the intrinsic viscosity of PDMC decreased steadily with increasing acetone (methanol)/water ratio. The jar test results with kaolin suspensions demonstrated the best clarification with PDMC as flocculant whilst the largest size of flocs obtained using PAM.