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Development of POSCO Application S/W Development Tool
Youngbok Park,JinHwi Lee,JinSeok Yang,kiWoung Park 제어로봇시스템학회 2011 제어로봇시스템학회 국제학술대회 논문집 Vol.2011 No.10
Whenever a work was revamped, we had replaced whole computer systems and developed all programs newly in spite of not changing functions and equipments. To save cost and time, we have tried to standardize the development process based on product line approach and construct the S/W asset to develop reusable components. We had developed S/W automatic development tool to assist the development and improve the productivity of S.W development. In this results, we could generate about 60% source code automatically and get platform independent application S/W to be reused easily in the next development.
갓 (Brassica juncea) 품종구분을 위한 ITS 영역 및 MITE Family 정보를 이용한 분자표지 개발
양기웅(Kiwoung Yang),이고은(Go-eun Yi),아리프 하산 칸 로빈(Arif Hasan Khan Robin),정남희(Namhee Jeong),이용혁(Yong-Hyuk Lee),박종인(Jongin Park),김회택(Hoyteak Kim),정미영(Mi-Young Chung),노일섭(Ill-Sup Nou) 한국원예학회 2016 원예과학기술지 Vol.34 No.2
갓(Brassica juncea; 2n = 4x = 36, AABB genome, 1,068Mb)은 U’s triangle의 배추와 흑겨자 사이의 복이배체 작물로 구분한다. 본 연구는 갓 15 품종의 ribosomal DNA ITS 영역과 MITE를 이용하여 갓의 유연관계 및 품종구분 분자표지를 확인하였다. Ribosomal DNA ITS 영역은 종 및 품종의 유연관계를 알아보는 연구로 많이 사용되고 있어서, 이를 이용하여 갓 15 품종의 유연관계를 알아보았다. 또한, MITE는 매우 많은 copy 수를 가지고 있고, 유전적으로 안정적이기 때문에 유전체 및 진화연구에 매우 적합한 재료이다. MITE를 이용한 갓의 품종구분 분자표지를 확인하기 위해 MITE super-families 중 Stowaway(BraSto) 관련 70점, Tourist(BraTo) 관련 79점, hAT(BrahAT) 관련 6점, Mutator(BraMu) 관련 5점으로 품종구분 표지를 알아보았다. 총 160점의 분자표지 중 32점이 갓 15 품종에서 뚜렷한 다형성을 보였다. 특히, 흑갓은 표현형뿐만 아니라 유전자형도 매우 다르게 나타났다. 또한 8점의 MITE 분자표지를 활용하여 47점의 유전자원에서 다형성 및 품종구분 표지로의 활용 가능성을 확인하였다. 이러한 다형성 표지들은 갓의 품종구분 및 품종 보호에 매우 유용하게 사용할 수 있을 것이라 기대한다. Brassica juncea (2n = 4x = 36, AABB genome, 1,068 Mb) is a U’s triangle species and an amphidiploid derivative of B. rapa and B. nigra. Fifteen varieties were used to study the ITS (internal transcribed spacer) regions of ribosomal DNA and MITEs (miniature inverted-repeat transposable elements) with a view of developing specific molecular markers. ITSs and MITEs are an excellent resource for developing DNA markers for genomics and evolutionary studies because most of them are stably inherited and present in high copy numbers. The ITS (ITS1 and ITS2) sequence was compared with the consensus sequence of B. rapa and B. nigra. Variation in ITS1 created two separate groups among 15 varieties, with 10 varieties in one group and 5 in the other. Phylogenetic analysis revealed two major clusters for those 10 and 5 varieties. Among the 160 different MITE primers used to evaluate the selected 15 varieties of B. juncea, 70 were related to the Stowaway, 79 to the Tourist, 6 to the hAT, and 5 to the Mutator super-families of MITEs. Of 160 markers examined, 32 were found to be polymorphic when fifteen different varieties of B. juncea were evaluated. The variety ‘Blackgat’ was different from the other mustard varieties with respect to both phenotype and genotype. The diversity of 47 additional accessions could be verified using eight selected molecular markers derived from MITE family sequences. The polymorphic markers identified in this study can be used for varietal classification, variety protection, and other breeding purposes.
Laila, Rawnak,Robin, Arif Hasan Khan,Yang, Kiwoung,Choi, Gyung Ja,Park, Jong-In,Nou, Ill-Sup MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.1
<P>Clubroot is a soil-borne disease caused by the protist <I>Plasmodiophora brassicae</I> (<I>P. brassicae</I>). It is one of the most economically important diseases of <I>Brassica rapa</I> and other cruciferous crops as it can cause remarkable yield reductions. Understanding <I>P. brassicae</I> genetics, and developing efficient molecular markers, is essential for effective detection of harmful races of this pathogen. Samples from 11 Korean field populations of <I>P. brassicae</I> (geographic isolates), collected from nine different locations in South Korea, were used in this study. Genomic DNA was extracted from the clubroot-infected samples to sequence the ribosomal DNA. Primers and probes for <I>P. brassicae</I> were designed using a ribosomal DNA gene sequence from a Japanese strain available in GenBank (accession number AB526843; isolate NGY). The nuclear ribosomal DNA (rDNA) sequence of <I>P. brassicae</I>, comprising 6932 base pairs (bp), was cloned and sequenced and found to include the small subunits (SSUs) and a large subunit (LSU), internal transcribed spacers (ITS1 and ITS2), and a 5.8s. Sequence variation was observed in both the SSU and LSU. Four markers showed useful differences in high-resolution melting analysis to identify nucleotide polymorphisms including single- nucleotide polymorphisms (SNPs), oligonucleotide polymorphisms, and insertions/deletions (InDels). A combination of three markers was able to distinguish the geographical isolates into two groups.</P>
Analysis of Aroma Compound Profiles in Doenjang (Soy Paste) Made from Twelve Different Soybeans.
Byong Won Lee,Tae Joung Ha,Kiwoung Yang,Jong Min Ko,Hyun Tae Kim,Young Hun Lee,Myeong Gi Jeon,Myeong Gi Jeon,Ha Sik Sim,Sang-Ouk Shin,Chan-Sik Jung,In Youl Baek,Keum Yong Park 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
Fermented soybean food is one of most economical and health food due to its valuable nutritional and medicinal attributes and have been consumed for centuries as flavoring ingredient in Korea. On fermented soybean food such as doenjang, aroma compounds are important property because they determine taste and grade of fermentation. This study investigated variety of aroma compounds of doenjang made from different soybean genotypes. Aroma compounds in twelve different doenjang made from two cultivar (Daewon and Taekwang) and ten elite lines were extracted by steam distillation extraction (SDE) method and analyzed by gas chromatography/mass spectrometry (GC/MS). Aroma compound were detected over 80 kinds in eight samples (Daewon, Taekwang, MY177, MY187, MY189, MY192, MY204 and MY205) and under 70 kinds in four samples (yeonchun1, MY188, MY203 and MY206). Among the detected aroma components, 47 compounds were assigned as aromatic compounds (21), long chain fatty acid (13), short chain fatty acid (5) and others (8) by the computer library (Wiley 7n program). The major aroma compounds in twelve different doenjang were benzaldehyde, benzeneacetaldehyde, 2-methoxy-4-vinylphenol, pyrazine type compounds, cysteaminesulfonic acid, palmitic acid, oleic acid and linoleic acid. Each doenjang made by same condition (microbe, temperature, aging time and etc) had a difference in ratio and variety of aroma compounds due to different components having soybean genotype.