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Iga, T.,Satoh, T.,Yamamoto, S.,Fukui, K.,Song, S.H.,Choi, K.C.,Roh, S.G.,Sasaki, S. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.11
Trans-10, cis-12 conjugated linoleic acid (CLA) has been reported to inhibit the adipocyte differentiation of preadipocytes in non-ruminant animals (mice, rat, and human). However, the effects of trans-10, cis-12 CLA have not been clear in ruminants. The objective of this study was to investigate the effects of trans-10, cis-12 CLA on adipocyte differentiation of ovine preadipocytes. Differentiation of these preadipocytes was facilitated by treatment with trans-10, cis-12 CLA. Trans-10, cis-12 CLA increased the number and size of oil red O-stainable lipid drops as well as the levels of GPDH activity. PPAR-$\gamma{2}$ and adipophilin mRNA, adipogenic marker genes, were increased by treatment with trans-10, cis-12 CLA. This result was different from that observed with 3T3-L1 preadipocytes, a clonal cell line derived from rodents. Furthermore, trans-10, cis-12 CLA alone induced the adipocyte differentiation of ovine preadipocytes in differentiation-induction medium without troglitazone. These results suggest that CLA is an inducer and regulator in adipocyte differentiation of ovine preadipocytes, with species differences between ovine and rodent preadipocytes.
Iga, Yuka,Konno, Tasuku Korean Society for Fluid machinery 2012 International journal of fluid machinery and syste Vol.5 No.1
In the turbopump inducer of a liquid propellant rocket engine, cavitation is affected by acceleration that occurs during an actual launch sequence. Since cavitation instabilities such as rotating cavitations and cavitation surges are suppressed during launch, it is difficult to obtain data on the influence of acceleration on cavitation instabilities. Therefore, as a fundamental investigation, in the present study, a three-blade cyclic cascade is simulated numerically in order to investigate the influence of acceleration on time-averaged and unsteady characteristics of cavitation that arise in cascade. Several cases of acceleration in the axial direction of the cascade, including accelerations in the upstream and downstream directions, are considered. The numerical results reveal that cavity volume is suppressed in low cavitation number condition and cavitation performance increases as a result of high acceleration in the axial-downstream direction, also, the inverse tendency is observed in the axial-upstream acceleration. Then, the regions in which the individual cavitation instabilities occur shift slightly to a low-cavitation-number region as the acceleration increases downstream. In addition, in a downstream acceleration field, neither sub-synchronous rotating cavitation nor rotating-stall cavitation are observed. On the other hand, rotating-stall cavitation occurs in a relatively higher-cavitation-number region in an upstream acceleration field. Then, acceleration downstream is robust against cavitation instabilities, whereas cavitation instabilities easily occur in the case of acceleration upstream. Additionally, comparison with the Froude number under the actual launch conditions of a Japanese liquid propellant rocket reveals that the cavitation performance will not be affected by the acceleration under the current launch conditions.