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Zhang Weixin,An Ning,Guo Junqi,Wang Zhixing,Meng Xiangfeng,Liu Weifeng 한국미생물학회 2021 The journal of microbiology Vol.59 No.4
The prominent protein producing workhorse Trichoderma reesei secretes a typical yellow pigment that is synthesized by a gene cluster including two polyketide synthase encoding genes sor1 and sor2. Two transcription factors (YPR1 and YPR2) that are encoded in the same cluster have been shown to regulate the expression of the sor genes. However, the physiological relevance of the yellow pigment synthesis in T. reesei is not completely clear. In this study, a yellow pigment hyper-producer OEypr1 and three yellow pigment non-producers, OEypr1-sor1, Δypr1, and OEypr2, were constructed. Their phenotypic features in mycelial growth, conidiation, cell wall integrity, stress tolerance, and cellulase production were determined. Whereas hyperproduction of the yellow pigment caused significant defects in all the physiological aspects tested, the non-producers showed similar colony growth, but improved conidiation, maintenance of cell wall integrity, and stress tolerance compared to the control strain. Moreover, in contrast to the severely compromised extracellular cellobiohydrolase production in the yellow pigment hyperproducer, loss of the yellow pigment hardly affected induced cellulase gene expression. Our results demonstrate that interfering with the yellow pigment synthesis constitutes an engineering strategy to endow T. reesei with preferred features for industrial application.
Xinxing Lv,Weixin Zhang,Guanjun Chen,Weifeng Liu 한국미생물학회 2015 The journal of microbiology Vol.53 No.4
Protein kinases are key players in controlling many basiccellular processes in almost all the organisms via mediatingsignal transduction processes. In the present study, we characterizedthe cellulolytic Trichoderma reesei orthologs ofSaccharomyces cerevisiae Sch9 and Yak1 by sequence alignmentand functional analysis. The T. reesei Trsch9Δ andTryak1Δ mutant strains displayed a decreased growth rateon different carbon sources and produced less conidia. Theabsence of these two kinases also resulted in different butabnormal polarized apical growth as well as sensitivity tovarious stresses. In addition, disruption of the genes Trsch9 orTryak1 resulted in perturbation of cell wall integrity. Interestingly,while the induced production of cellulases was slightlycompromised in the Trsch9Δ strain, the extracellular productionof cellulases was significantly improved in the absenceof Yak1. The results indicate that TrSch9 and TrYak1play an important role in filamentous growth, stress responseand induced production of cellulases in T. reesei.
Lanzi Cheng,Rui Zhang,Weixin Lv,Luyu Shao,Zheng Wang,Wei Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2020 NANO Vol.15 No.03
Highly efficient, cost-effective and durable electrocatalysts for water splitting are crucial for energy conversion and storage. Transition-metal phosphides have been proven to be efficient catalysts for water splitting. In this paper, surface phosphation of 3D NiCo2O4 nanowires grown on Ni foam (P-NiCo2O4/NF) have been prepared to investigate the effect of surface phosphating on catalyst activity. XRD and XPS results demonstrate that P element has been decorated on the surface of the NiCo2O4 nanowires. The electrochemical results prove that P-NiCo2O4/NF shows better electrochemical performance than pure NiCo2O4/NF as an electrode for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) of water splitting. It achieves a current density of 10 mA cm2 at an overpotential of 279 mV and 164 mV for OER and HER in 1.0 M KOH electrolyte, respectively. In addition, the P-NiCo2O4/NF || P-NiCo2O4/NF electrode is constructed by employing P-NiCo2O4/NF as both the anode and cathode, it only requires a low 1.68 V of cell voltage to reach the current density of 10 mA cm -2. Notably, P-NiCo2O4/NF||P-NiCo2O4/NF also exhibits excellent stability for over 30 h-long. These results indicate that surface phosphation is an effective approach to improve the electrochemical performance of NiCo2O4/NF electrode materials.
Jun Wang,Sufang Huo,Yuxiu Zhang,Yaping Liu,Weixin Fan 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.3
Wines produced by three different grape varieties were treated with seven pre-fermentation techniques to analyze their variation in polyphenolic compounds, color parameters, and aromatic components. For polyphenolic compounds and color parameters, a better result was obtained after the grape pulp treatment, with the maximum impact observed after joint treatment with thermovinification and pectolytic enzymes, followed by thermovinification and cold soak, while pectolytic enzymes treatment alone was ineffective, resulting in almost no effect. On the contrary, the application of thermovinification and cold soak treatments before crushing the whole juice led to a dramatic decline in most indexes. For aromatic components, the joint treatment of thermovinification and pectolytic enzymes on grape pulp significantly affected its amount and type, in addition to giving the wine a mellow aroma.